RESUMO
Mitoviruses were initially known for their presence in the mitochondria of fungi and were considered exclusive to these organisms. However, recent studies have shown that they are also present in a large number of plant species. Despite the potential impact that mitoviruses might have on the mitochondria of plant cells, there is a lack of information about these ancient RNA viruses, especially within the Cannabaceae family. Cannabis sativa has been in the spotlight in recent years due to the growing industrial applications of plant derivatives, such as fiber and secondary metabolites. Given the importance of Cannabis in today's agriculture, our study aimed to expand the knowledge frontier of Mitoviruses in C. sativa by increasing the number of reference genomes of CasaMV1 available in public databases and representing a larger number of crops in countries where its industrial-scale growth is legalized. To achieve this goal, we used transcriptomics to sequence the first mitoviral genomes of Colombian crops and analyzed RNA-seq datasets available in the SRA databank. Additionally, the evolutionary analysis performed using the mitovirus genomes revealed two main lineages of CasaMV1, termed CasaMV1_L1 and CasaMV1_L2. These mitoviral lineages showed strong clustering based on the geographic location of the crops and differential expression intensities.
Assuntos
Cannabis , Vírus de RNA , Cannabis/genética , Filogenia , Vírus de RNA/genética , Mitocôndrias/genética , FungosRESUMO
Feces-contaminated water is a vehicle of transmission of potentially pathogenic microorganisms responsible for illnesses that represent main causes of death worldwide. A protocol for detection of intestinal parasites in great volumes of water was optimized. It includes: membrane filtration, mechanical agitation with detergent, centrifugation, chemical concentration with Mini Parasep® and microscopic examination. From samples of feces-contaminated water containing parasitic forms, a total recovery percentage of 85.7 percent of parasites was achieved after tests. This procedure provides a useful alternative method that could be subjected to validation as a routine methodology in the diagnosis of microbiological water quality(AU)
El agua contaminada con heces es un vehículo de transmisión de microorganismos potencialmente patógenos causantes de enfermedades que constituyen causas principales de muerte a nivel mundial. Se optimizó un protocolo para la detección de parásitos intestinales en grandes volúmenes de agua. Este incluye: filtración por membrana, agitación mecánica con detergente, centrifugación, concentración química con Mini Parasep® y examen microscópico.En muestras de agua contaminada con heces que contenían formas parasitarias, se obtuvo un porcentaje de recuperación total del 85.7 por ciento de estas formas después de aplicar el protocolo. El procedimiento constituye un método alternativo que podría someterse a validación como metodología habitual para el diagnóstico de la calidad microbiológica del agua(AU)
