RESUMO
Objective To investigate the prevalence and risk factors of hyperuricemia in physical examination group of Zhangjiakou, and to provide evidence for the prevention of hyperuricemia. Methods The physical examination people in the Number One Hospital of Zhangjiakou were selected as the research objects from May 2016 to May 2017 . Their physique indexes such as height, weight and blood pressure were tested, and their blood sample were collected and biochemical indexes such as fasting plasma glucose (FPG), blood uric acid (UA), triglyceride (TG) and total cholesterol (TC) were detected. Logistic regression model were used to analyze the influencing factors of hyperuricemia. Results A total of 17273 examinees were recruited, with the average age of (47.42±14.28) years. There were 10324 men (59.77％) and 6949 women (40.23％) . The prevalence rate of hyperuricemia was 22.14％. The prevalence rate of hyperuricemia in men was 28.39％, higher than that of women (12.87％) (P<0.001) . Logistic regression analysis showed that obesity/overweight (OR=2.206, 95％ CI: 1.986-2.450), high TG (OR=2.089, 95％CI: 1.903-2.293) and high TC (OR=1.121, 95％CI: 1.006-1.249) were risk factors of the male patients with hyperuricemia, while the elderly (OR=0.982, 95％CI: 0.979-0.985) and high FPG (OR=0.657, 95％ CI: 0.587-0.736) were protective factors. The risk factors of the female patients with hyperuricemia were the elderly (OR=1.008, 95％CI: 1.001-1.014), obesity/overweight (OR=2.193, 95％CI: 1.864-2.579), high FPG (OR=1.379, 95％CI: 1.128-1.687) and high TG (OR=2.209, 95％ CI: 1.879-2.597) . Conclusion The prevalence of hyperuricemia among residents in Zhangjiakou of Hebeiis high. Overweight / obesity and high TG are the risk factors for hyperuricemia.
RESUMO
OBJECTIVE: To explore the effects of cyclopamine on the proliferation and apoptosis of LNCaP cells and the expression of the PCA3 gene in human prostate cancer in vitro. METHODS: LNCaP cells were treated with cyclopamine at the concentrations of 1, 5, 10 and 15 micromol/L for 24, 48 and 72 hours. The inhibitory effects of cyclopamine on the proliferation and apoptosis of the LNCaP cells were detected by MTT and flow cytometry respectively, the morphological changes of the cells observed by Hoechst 33258 staining, and the expression of the PCA3 gene determined by real-time fluorescence quantitative reverse transcriptase polymerase chain reaction (FQ-RT-PCR). RESULTS: Compared with the blank control group, cyclopamine significantly inhibited the proliferation of the LNCaP cells at 5, 10 and 15 micromol/L (P <0.01), reaching IC50 at 10 micro mol/L at 48 hours. The apoptosis rates of the LNCaP cells at 24, 48 and 72 hours were 37.21%, 57.38% and 57.98% in the 10 micromol/L group and 21. 16% , 71.31% and 72.90% in the 15 micro.mol/L group, significantly different from those in the control (P <0. 01). The cell apoptosis showed a rising trend with the increase of cyclopamine concentration and acting-time, while the expression of the PCA3 gene was decreasing with the increased concentration of cyclopamine, significantly lower than that of the blank control group (P <0.01) , and extremely low in the 10 micromo/L group CONCLUSION: Cyclopamine intervention at 10 and 15 micromol/L for 48 and 72 hours could significantly inhibit the at all time points. Proliferation and induce the apoptosis of LNCaP cells and reduce the expression level of PCA3.
