Research progress on the molecular mechanism of periodontal pathogens promoting the development of cardiovascular diseases / 口腔疾病防治

@#Periodontitis is a multifactorial infectious and inflammatory disease occurring in tooth-supporting tissues. In recent decades, many studies have reported a potential relationship between periodontitis and cardiovascular disease, and periodontal pathogens are an important factor linking periodontitis and cardiovascular disease. In this review, we summarize updated preclinical studies and epidemiological evidence on the association of these two diseases. Moreover, possible mechanisms accounting for such links are introduced, including bacteremia and direct invasion of pathogens, endotoxemia caused by virulence factors of periodontal pathogens leading to systemic inflammation, abnormal lipid metabolism and oxidative stress, which further affect the inflammatory states of the cardiovascular system. The molecular mimicry theory and the intrinsic correlation of apolipoprotein E between periodontitis and cardiovascular disease require further study. Combined with existing studies, it is reasonable to assume that periodontal treatment and oral hygiene can reduce the risk of cardiovascular disease in patients with periodontitis. More studies are needed to focus on the molecular mechanism linking periodontal pathogens and cardiovascular diseases. These studies will provide evidence that periodontal pathogens directly invade the cardiovascular system or indirectly invade host cells as well as isolate and culture bacteria from the tissues of lesions. Studies should also explore how the local inflammatory state, periodontal pathogens and their products directly influence cardiovascular disease-related biomarkers (C-reactive protein, vascular endothelial growth factor, heat shock protein, etc.) and the mechanism. This information may provide a reference for the effective prevention and treatment of periodontitis and cardiovascular disease in the future.

Dental calculus: A repository of bioinformation indicating diseases and human evolution.

Dental calculus has long been considered as a vital contributing factor of periodontal diseases. Our review focuses on the role of dental calculus as a repository and discusses the bioinformation recently reported to be concealed in dental calculus from three perspectives: time-varying oral condition, systemic diseases, and anthropology at various times. Molecular information representing an individual's contemporary oral health status could be detected in dental calculus. Additionally, pathogenic factors of systemic diseases were found in dental calculus, including bacteria, viruses and toxic heavy metals. Thus, dental calculus has been proposed to play a role as biological data storage for detection of molecular markers of latent health concerns. Through the study of environmental debris in dental calculus, an overview of an individual's historical dietary habits and information about the environment, individual behaviors and social culture changes can be unveiled. This review summarizes a new role of dental calculus as a repository of bioinformation, with potential use in the prediction of oral diseases, systemic diseases, and even anthropology.

Sparse Bayes Tensor and DOA Tracking Inspired Channel Estimation for V2X Millimeter Wave Massive MIMO System.

Efficient vehicle-to-everything (V2X) communications improve traffic safety, enable autonomous driving, and help to reduce environmental impacts. To achieve these objectives, accurate channel estimation in highly mobile scenarios becomes necessary. However, in the V2X millimeter-wave massive MIMO system, the high mobility of vehicles leads to the rapid time-varying of the wireless channel and results in the existing static channel estimation algorithms no longer applicable. In this paper, we propose a sparse Bayes tensor and DOA tracking inspired channel estimation for V2X millimeter wave massive MIMO system. Specifically, by exploiting the sparse scattering characteristics of the channel, we transform the channel estimation into a sparse recovery problem. In order to reduce the influence of quantization errors, both the receiving and transmitting angle grids should have super-resolution. We obtain the measurement matrix to increase the resolution of the redundant dictionary. Furthermore, we take the low-rank characteristics of the received signals into consideration rather than singly using the traditional sparse prior. Motivated by the sparse Bayes tensor, a direction of arrival (DOA) tracking method is developed to acquire the DOA at the next moment, which equals the sum of the DOA at the previous moment and the offset. The obtained DOA is expected to provide a significant angle information update for tracking fast time-varying vehicular channels. The proposed approach is evaluated over the different speeds of the vehicle scenarios and compared to the other methods. Simulation results validated the theoretical analysis and demonstrate that the proposed solution outperforms a number of state-of-the-art researches.

[Expression, purification and interaction of human leukocyte antigen F and cluster of differentiation 8alpha homodimers].

To obtain large quantity of human leukocyte antigen F (HLA-F) and cluster of differentiation 8alpha homodimers (CD8alphaalpha) proteins and to study their relationship, HLA-F and CD8alpha genes with rare codon in Escherichia coli were cloned using an N-terminal synonymous mutation method. High-efficiency expression protein inclusion bodies were acquired. The proteins were refolded using the dilution method and purified with gel-filtration and anion exchange chromatography. The results of gel-filtration and native-PAGE indicate that HLA-F interacts with CD8alphaalpha. This interaction may affect the binding between CD8alphaalpha and other MHC molecules to regulate immune responses. These results provide a basis for further research of HLA-F.