RESUMO
An immunohistochemical evaluation of Le(a)-Le(x) expression by adenocarcinomas of the biliary tree, pancreas, colon and stomach was undertaken as examples of epithelial tumors derived from embryonic endoderm. This complements previous studies showing that Lea-Lex was present on the cell surface of non-small cell lung carcinomas, some non-lung carcinomas, and is a prognostic marker for squamous cell lung carcinomas. All of the tumor specimen evaluated were positive and no expression of Le(a)-Le(x) was detected in derivatives of neural, connective or muscle tissues. These findings indicate that it could be informative to examine the biological significance of Le(a)-Le(x) not only in carcinogenesis but during embryogenesis, as well.
Assuntos
Adenocarcinoma/patologia , Biomarcadores Tumorais/análise , Oligossacarídeos/análise , Adenocarcinoma/cirurgia , Carcinoma Pulmonar de Células não Pequenas/patologia , Neoplasias do Colo/patologia , Embrião de Mamíferos , Endoderma , Neoplasias da Vesícula Biliar/patologia , Mucosa Gástrica/patologia , Humanos , Imuno-Histoquímica/métodos , Mucosa Intestinal/patologia , Antígenos do Grupo Sanguíneo de Lewis , Antígenos CD15 , Neoplasias Pulmonares/patologia , Metaplasia , Neoplasias de Células Escamosas/patologia , Neoplasias Pancreáticas/patologia , Prognóstico , Neoplasias Gástricas/patologiaRESUMO
Intratumoral phenotypic diversity is well documented with regard to tumor associated carbohydrate antigens (TACA). The factors which control the expression of these cell-surface oligosaccharides on different cells of the same tumor are not understood. We investigated the expression of a panel of mucin associated oligosaccharides in cell lines growing at different surface densities (number of cells per cm2 of growth flask). Results show that the apparent expression of extended Lea-Lex, Lea and Lex, sialyl Lea, Tn and sialyl Tn varies with density of growth by an invasive human squamous cell lung carcinoma cell line (NU6-1), a benign variant (NE-18) and the human lung epithelial cell line BEAS-2B. The results indicate that one of the factors influencing the apparent expression of mucin-associated oligosaccharides is cell-cell interactions.
Assuntos
Glicoproteínas de Membrana/metabolismo , Mucinas/metabolismo , Anticorpos Monoclonais/imunologia , Anticorpos Monoclonais/metabolismo , Antígenos Glicosídicos Associados a Tumores/imunologia , Antígenos Glicosídicos Associados a Tumores/metabolismo , Divisão Celular , Células Clonais , Epitopos/química , Epitopos/genética , Regulação Neoplásica da Expressão Gênica/genética , Humanos , Immunoblotting , Imuno-Histoquímica , Antígenos do Grupo Sanguíneo de Lewis/análise , Antígenos do Grupo Sanguíneo de Lewis/metabolismo , Neoplasias Pulmonares/metabolismo , Microscopia de Contraste de Fase , Mucinas/imunologia , Oligossacarídeos/química , Oligossacarídeos/metabolismo , Células Tumorais CultivadasRESUMO
The extended Le(a)-Le(x) oligosaccharide, expressed as a cell surface antigen by human squamous lung carcinomas marks cancer cells of tumors having poor prognosis. In order to see if the extended Le(a)-Le(x) also has a precisely controlled pattern of expression during embryogenesis, a survey of representative vertebrate embryos and fetuses in various stages was undertaken. Embryonic and fetal cells which express the epitope are derived from embryonic endoderm. No. epitope expression was demonstrated in tissues of ectodermal or mesodermal origin. Vertebrate conservation of this endodermal cell surface carbohydrate suggests function necessary for normal growth and development with inappropriate re-expression during carcinogenesis.
