Human papillomavirus detection using in situ hybridization and correlations with histological and cytological findings.

BACKGROUND AND OBJECTIVE: The human papillomavirus (HPV) is the main causative agent of cancerous and precancerous lesions in cervical cancers. The infection detection is performed by cytological and histological examination. However, molecular testing for HPV infection remains necessary to distinguish nonspecific inflammatory lesions from HPV-related lesions. We aimed to compare cytological and histological diagnosis of HPV lesions and to confront histological findings with molecular detection of HPV by in situ hybridization (ISH). MATERIAL AND METHODS: A total of 2399 pap smears were collected between 1997 and 2002. Twenty-nine of them presented with lesions indicative of HPV infection. Colposcopy and biopsy were performed on 23 of these 29 pap smears, with a comparison of the cytological and histological findings. Molecular testing for HPV by ISH was performed on 11 pap smears. RESULTS: A 79% cytological and histological agreement was observed. ISH revealed the presence of the virus in seven case patients, amounting to a 63% histological and molecular agreement. Seven HPV genotypes were categorized (6-11-16-18-31-33-51). CONCLUSION: HPV detection by ISH adds little to the diagnosis of HPV-associated lesions based on cytological and histological features. It, however, helps improve the specificity of the diagnosis and determine the viral genotype which, in turn, helps better define the lesion prognosis.

Intraosseous hibernoma: a case report and review of the literature.

Intraosseous hibernoma is a rare benign bone tumor, with only 4 cases reported in English literature. CASE REPORT: In this report, we describe a 42-year-old man with  no past medical history and right chest pain. Imaging studies showed an osteolytic lesion with fat attenuation lesion in the right fourth rib. The lesion was completely resected and microscopic study showed mildly thickened bone trabeculae and multivacuolated brown fat cells replacing the normal white fat and hematopoietic elements. The diagnostic of intraosseous hibernoma was made. CONCLUSION: Although it is rare, hibernoma should be included in the differential diagnosis of lipomatous  tumors.

Intraosseous hibernoma: a case report and review of the literature.

Intraosseous hibernoma is a rare benign bone tumor, with only 4 cases reported in English literature. CASE REPORT: In this report, we describe a 42-year-old man with  no past medical history and right chest pain. Imaging studies showed an osteolytic lesion with fat attenuation lesion in the right fourth rib. The lesion was completely resected and microscopic study showed mildly thickened bone trabeculae and multivacuolated brown fat cells replacing the normal white fat and hematopoietic elements. The diagnostic of intraosseous hibernoma was made. CONCLUSION: Although it is rare, hibernoma should be included in the differential diagnosis of lipomatous  tumors.

Amyloid goiter: first manifestation of systemic amyloidosis.

Amyloidosis is an abnormal extracellular deposit, which can occur in several tissues. The mechanism is not clearly defined. In systemic amyloidosis, all the organs can be infiltrated, but amyloid goiter as the initial manifestation of systemic amyloidosis is an exceedingly rare condition. We report a rare case of a patient who presented an amyloid goiter as the first manifestation of systemic amyloidosis. This patient had a known Crohn's disease. He developed a goiter without compressive complications. Histologic examination revealed a diffuse amyloid deposition surrounding thyroid follicles. The gland was enlarged with an eosinophilic and amorphic deposit. Confirmation of amyloid was made by the presence of congophilia and apple-green birefringence under polarized-light microscopy. An immunoreactivity was seen with AA protein. Amyloid goiter is a rare manifestation of amyloidosis. About 250 cases of amyloid goiter have been reported in the literature. The goiter enlarges rapidly and progressively, often becoming compressive like thyroid cancer. The prognosis depends on the treatment of the amyloidosis and the underlying chronic disease.

[Intracerebral schwannoma: case report]. / Schwannome intracérébral: étude anatomoclinique d'une observation.

BACKGROUND AND PURPOSE: We report a well-documented case of intracerebral schwannoma, presenting as a cystic and solid parietal mass, arising in a 20-year-old woman. Possible mechanisms underlying the histogenesis of this rare lesion are discussed. METHODS: The clinical, radiological and pathological features are described. The relevant literature is reviewed. RESULTS: The patient presented seizures and elevated intracranial pressure. Neuroradiologic findings showed a right parietal lesion with cystic and tissular components, intensely enhanced after injecting intravenous gadolinium. The tumor was removed through a right parietal craniotomy. Histological and immunochemical features confirmed the diagnostic of intracerebral schwannoma. The patient is alive without progressive local disease or metastasis. The origin of intracerebral schwannomas has been the source of much controversy. The most popular hypothesis argues that these tumors arise from the proliferation of Schwann cells in the perivascular nerve plexii. CONCLUSIONS: Intracerebral schwannoma is an extremely rare benign tumor. About fifty cases have been reported. The importance of recognizing this tumor is stressed, particularly in younger patients, given its benign nature, radiological resemblance to other tumors and favorable response to resection without toxic treatment.

[Epithelial-myoepithelial carcinoma of the nasal cavity]. / Carcinome épithélial-myoépithélial de la cavité nasale.

OBJECTIVES: Epithelial-myoepithelial carcinoma is a rare tumor of the salivary glands with an incidence of less than 1%. Most cases arise in the parotid gland. Extraoral location is exceptional. The purpose of this study was to describe a case with nasal location, which is exceptionally reported in the literature. MATERIALS AND METHODS: We report a case of epithelial-myoepithelial carcinoma arising in the nasal cavity of a 54-year-old woman. RESULTS: The woman presented with right recurrent epistaxis and on nasal endoscopic examination was found to have a polypoid tumor in the right nasal cavity. The CT-scan demonstrated a soft tissue mass without extension to the rhinopharynx or bony destruction. Histopathologic examination revealed the tumor to consist of a mixture of a solid, tubular, and trabecular structures with a double-layered arrangement of inner eosinophilic cells and outer clear cells. Dual differentiation toward myoepithelial and epithelial cells was confirmed immunohistochemically. CONCLUSION: The occurrence of epithelial-myoepithelial carcinoma in the nasal cavity is possible. In published cases, no recurrence or metastasis has been reported in this location.

[Sclerosing hemangioma of the lung: a rare lesion with a difficult diagnosis]. / L'hémangiome sclérosant du poumon: une lésion rare et de diagnostic difficile.

Sclerosing hemangioma of the lung is a rare lesion described for the first time in 1956 by Liebow. We report a case in a 45 year-old woman who was admitted for exploration of chronic cough. The chest x-ray revealed a round opacity, well delimited in the left pulmonary parenchyma. Surgical resection enabled the histopathological diagnosis of sclerosing hemangioma. We review progress in our knowledge of the histogenesis and diagnosis of this tumor. Immunohistochemistry has been highly contributory although numerous points remain controversial.

[Detection of Epstein-Barr virus in Malt type gastric malignant lymphoma using in situ hybridization]. / Recherche du virus d'Epstein Barr dans les lymphomes malins de type Malt gastriques par hybridation in situ.

The aim of our study was to look for Epstein Barr virus (EBV) genome by in situ hybridization in 23 MALT gastric lymphomas. 15 cases were of low grade, and 8 were of high grade malignancy. We obtained a positive result in 3 cases (13%) of low grade lymphomas. EBV was present in few centrocyte-like and centroblastic cells. Literature review shows an EBV infection rate varying between 8 and 12% with variables results which don't allow strong and reliable conclusion. An etiopathogenic role for EBV in Malt gastric lymphoma remain yet hypothetic.