Restriction analyze of starch synthesis genes in Amaranth mutant lines.

The increase interest and study of underutilized and neglected crops is actual for the last years. In the study, the restriction polymorphisms of GBSSI and SSSI gene in gamma-irradiated mutant lines of amaranth was performed. To evaluate polymorphisms of restriction sites endonucleases AciI, BsaJI, FatI EcoRI, BamHI, PstI, HpaII and PciI were used. GBSSI gene responsible to amylose synthesis was digested with HpaII and PciI restriction endonucleases. Two PciI restriction sites and three HpaII restriction sites were evaluated and any changes in restriction sites were recorded. Profile changes of the SSSI gene in mutant lines C 15/1 and C 236/1 were recorded after the restriction digest by BsaJI. Restriction cleavage polymorphism was recorded after the restriction digest of segment 5250-6854 bp with restriction endonuclease FatI, too.

Effect of cultivation medium on some physicochemical parameters of outer bacterial membrane.

The changes of surface charge and hydrophobicity of the outer bacterial membrane in relation to utilization of n-hexadecane were studied. For this spectrophotometric study adsorption of methylene blue and transport of gentian violet were used. The decrease in the negative charge of the bacterial strains Pseudomonas putida CCM 3423, P. aeruginosa, and P. fluorescens CCM 2115, depended on the type of growth medium. The decrease of surface charge was in the order: meat extract peptone broth > mineral medium with glucose > mineral medium with n-hexadecane. The highest permeability of the bacterial membrane for gentian violet was determined in the case of P. fluorescens grown in meat extract peptone broth. This effect can be explained by a greater hydrophobicity of the bacterial surface for this strain. In other strains a lower permeability was observed. P. fluorescens showed a greater adherence to hexadecane.