Accumulation of metals in cancerous and healthy tissues of patients with lung cancer in Southern Poland.

The aim of this study was to investigate whether the concentrations of metals differ among patients with and without lung cancer with different smoking status and living in industrialized environments. We also evaluated the relationships between metals and blood parameters including hematocrit level (Hct), hemoglobin concentration (Hb), red (RBC) and white (WBC) blood cells numbers. Concentrations of metals were measured with AAS (copper - Cu, iron - Fe, magnesium - Mg, zinc - Zn) and CV-AAS (mercury - Hg). Neither smoking status nor industrialization could be considered as a significant factor for metals accumulation in blood, lungs and tumor tissues, with the exception of mercury which differed in the aspect of industrialization. According to the type of the disease, Fe, Hg and Mg concentrations differed significantly in lungs. Correlations between metals and blood parameters were observed. Additionally, concentrations of Mg, Cu and Zn were correlated between lungs and tumor tissue of patients with cancer as well as they all were related to each other in lungs, tumor and blood tissues.

Mercury concentrations in human placenta, umbilical cord, cord blood and amniotic fluid and their relations with body parameters of newborns.

Studies were conducted on samples taken from giving birth women (n = 40) living in Poland, representing three age groups: 19-25, 26-30 and 31-38 years old. Mercury concentrations were measured with CV-AAS in placenta, umbilical cord, cord blood and amniotic fluid. The placentas weight did not exceed the 750 g value and was heavier than 310 g. Mean values of Hg concentrations in blood, placenta and umbilical cord were similar (c.a. 9 µg/g). High levels of mercury were noted in cord blood which in 75% of all observations exceeded (up to 17 µg/L) the safe dose set by US EPA (5.8 µg/L). No statistically significant differences in medium level of Hg in all the studied tissues among age groups of women were observed. Positive correlations between Hg concentrations in placenta and umbilical cord and cord blood were revealed as well as some negative ones between mercury concentrations and pregnancy parameters.

Accumulation of zinc, nickel, lead and cadmium in some organs of rabbits after dietary nickel and zinc inclusion.

This study reports the effect of dietary nickel (Ni) and a combination of Ni and zinc (Zn) on the accumulation of lead (Pb), cadmium (Cd), Ni and Zn in muscles, liver and kidneys of rabbits. Female rabbits (Oryctolagus cuniculus) of experimental groups were fed a granular mixture with addition of various concentrations of Ni or Ni+Zn (E1 - 17.5 g NiCl(2) per 100 kg of feed mixture, group E2 - 35.0 g NiCl(2) per 100 kg of feed mixture, group E3 - 17.5 g NiCl(2) per 100 kg of feed mixture and 30 g ZnCl(2) per 100 kg of feed mixture, group E4 - 35.0 g NiCl(2) per 100 kg of feed mixture and 30 g ZnCl(2) per 100 kg of feed mixture). Group C without inclusion of Ni or Zn served as control. After the 90-day experimental period biological material (liver, kidney, musculus longissimus dorsi, musculus biceps femoris) was taken from the animals. Samples were analyzed by the AAS method. Ni added to the FM caused an increase in Cd concentration in the kidneys of the rabbits, significantly in the E3 group in comparison with the control group. In the liver an insignificant decrease of Cd concentration was found. Zn addition in the amount of 30 g to the diet caused an increase of Cd level in the kidney as well as in the liver. Ni and Zn treatment caused a significant decrease of Pb accumulation in the m. longissimus dorsi of rabbits. This study indicates that dietary inclusion of Ni and Zn caused specific interactions among the observed metals.

Scots pine bark, topsoil and pedofauna as indicators of transport pollutions in terrestrial ecosystems.

The impact of the motorway on pollution was evaluated by determining chosen heavy metals and acid reaction (pH) in the pine bark, in forest and meadow topsoil. The content of these environmental contaminants was determined in the topsoil and in the bark of around 40 year-old Scots pines (Pinus sylvestris L.) growing right next to the soil analyzed. The pollutants were examined at localities situated around 5, 200, 1500 m away from the motorway. To evaluate influence of Cd, Pb, Zn, Cu and the topsoil pH on pedofauna, five meadows localities situated 1, 20, 40, 150, 1550 m away from the motorway were examined. It was detected that in the forest habitats analyzed the bark was characterized by considerably higher acidity (pH 3.14-3.88) than the topsoil of the pines analyzed (pH 5.45-7.22). Except of Cd at locality 200 m and Cu at 1500 m from motorway, the higher concentration of heavy metals was noted in topsoil. In the meadow soil of the locality 150 m from the motorway the highest concentrations of Cd and Zn were detected. The greatest diversity of the meso and macrofauna and trophic relations the most resembling natural were detected in the area furthest away from the motorway, where the content of the heavy metals was the lowest. The lowest density and diversity of meso- and macrofauna were detected in the area situated 40 m, where the concentration of heavy metals was higher than at 1, 20 and 1550 m from the motorway situated localities.

Cadmium availability to freshwater mussel (Unio tumidus) in the presence of organic matter and UV radiation.

The influence of ultraviolet radiation (UV) on cadmium availability to freshwater mussel (Unio tumidus) in the presence of organic matter was investigated. Water solutions containing 1000, 500, and 250 microg x L(-1) of cadmium and 28.8 mg x L(-1) of total organic carbon (TOC) were exposed to ecologically relevant biologically effective UV-A and UV-B irradiances of 1.73 x 10(-6) W x cm(-2) and 1.50 x 10(-5) W x cm(-2) respectively for 12 h. The availability of cadmium was measured directly as Cd concentration in gills, mantle, digestive gland and foot, and indirectly as its impact on the content of essential cations. The concentration of cadmium ions, soluble forms of cadmium, pH and conductivity in UV irradiated solutions were also measured. UV exposures resulted in a decrease of the TOC to 25.9 mg x L(-1), followed by a decrease in the total concentration of Cd soluble forms, increase in the concentration of cadmium bound to particulate matter, and increase in the concentration of free cadmium ions in comparison to non-irradiated mixtures. Mussels from mixtures exposed to UV accumulated more cadmium than specimens from non-irradiated mixtures. This was confirmed by the fluctuations of essential cations, i.e. decreases in the contents of Zn, Ca, Fe and increase of the Cu content. Na/K molar ratios in gills were negatively correlated with cadmium content, while a positive trend occurred in other organs. We suggest that in UV-exposed water more ionic forms of cadmium occur. On the other hand more cadmium is also bound to particulate matter. Our research indicate that the increase of cadmium accumulation in mussels grown in humic acid solutions exposed to UV were most probably related to the increase in the concentration of cadmium bound to particulate matter.