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1.
Science ; 304(5677): 1665-9, 2004 Jun 11.
Artigo em Inglês | MEDLINE | ID: mdl-15192228

RESUMO

Before European contact, Hawai'i supported large human populations in complex societies that were based on multiple pathways of intensive agriculture. We show that soils within a long-abandoned 60-square-kilometer dryland agricultural complex are substantially richer in bases and phosphorus than are those just outside it, and that this enrichment predated the establishment of intensive agriculture. Climate and soil fertility combined to constrain large dryland agricultural systems and the societies they supported to well-defined portions of just the younger islands within the Hawaiian archipelago; societies on the older islands were based on irrigated wetland agriculture. Similar processes may have influenced the dynamics of agricultural intensification across the tropics.

2.
Electrophoresis ; 18(9): 1534-7, 1997 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-9378117

RESUMO

The human colonisation of Polynesia was a major event in world prehistory. It represents one of the last human population migrations, and one which required crossing major water barriers. Though the subject of Pacific population origins has been approached by scholars from numerous fields for nearly a century, recent years have seen the problem addressed by human geneticists. Since the initial report describing the recovery of DNA from skeletal remains, ancient DNA studies have also focused on the Pacific region. In this paper we present the results of ancient DNA analyses of Rattus exulans, an animal that was transported by ancestral Polynesians through the Pacific to the far reaches of the Polynesian triangle. Analysis of DNA of R. exulans skeletal remains has many advantages over studies of ancient human remains, yet the one drawback has been the recovery of ancient DNA from single bones of these very small rodents. We have successfully modified standard extraction protocols for ancient DNA and have consistently extracted, amplified and sequenced mitochondrial DNA (mtDNA) from less than 0.1 g of R. exulans bone and tooth samples recovered from archaeological sites throughout the Pacific, ranging from 400 to 2000 years old.


Assuntos
Osso e Ossos/química , DNA/análise , DNA/química , Fósseis , Muridae/genética , Análise de Sequência de DNA , Animais , Sequência de Bases , DNA Mitocondrial/análise , DNA Mitocondrial/química , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Polinésia , Ratos
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