RESUMO
To prepare polyrotaxane-camptothecin conjugates and evaluate its anti-tumor effect, polyrotaxane-camptothecin conjugates were successfully synthesized, and the release behavior was performed; MTT assay and cell morphology were used to examine the inhibition of cells' proliferation effect in vitro. The experimental study of the antitumor effect on S180 mice in vivo was also performed to further evaluate the anti-tumor effect of conjugate. The result showed polyrotaxane-camptothecin conjugates can effectively inhibit the proliferation in a dose dependent effect. In vivo study and cell morphology observation of S180 mice showed significant decrease in growth of tumor, degree of tumor infiltration and blood vessel number. The result indicated anti-tumor mechanism may be through affect the angiogenesis and reduced blood supply to tumor cells and then leading to necrosis.
Assuntos
Antineoplásicos Fitogênicos/farmacologia , Camptotecina/farmacologia , Proliferação de Células/efeitos dos fármacos , Ciclodextrinas/química , Neoplasias Ovarianas/patologia , Poloxâmero/química , Rotaxanos/química , Animais , Antineoplásicos Fitogênicos/administração & dosagem , Camptotecina/administração & dosagem , Linhagem Celular Tumoral , Portadores de Fármacos , Composição de Medicamentos , Feminino , Humanos , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Transplante de Neoplasias , Sarcoma 180/patologia , Carga Tumoral/efeitos dos fármacosRESUMO
AIM: To establish a preparation method of cartridge for gene gun and different gene transfection to MVF-7 cell lines in vitro. METHODS: The cartridge was prepared by precipitation method of spermidine and calcium chloride.using gene gun method, respectively, the eukaryotic expression plasmid pEGFP, Pmcherry transfected with the control group and experimental group MCF-7 cells 24 h after transfection. RESULTS: The preparation of the gene-gun bullets, gene gun-mediated pEGFP, Pmcherry be able to separate and co-transfected into cultured MCF-7 cells, 24 h after transfection could be detected in red, green fluorescence, while the control group there was no fluorescent protein expression. CONCLUSION: Gene gun can be effectively mediated gene transfer and be able to achieve a common transfer of two genes. The report gene can be expressed through gene gun-transfected MCF-7 cells.
Assuntos
Biolística/métodos , DNA/genética , Células Eucarióticas/metabolismo , Plasmídeos/genética , Transfecção/métodos , Linhagem Celular Tumoral , Humanos , Mapeamento por RestriçãoRESUMO
OBJECTIVE: To optimize the preparation technology of GBE 50-phospholipid complex and study its physicochemical properties. METHODS: The preparation conditions for GBE 50-phospholipid complex were optimized by means of single factor study and orthogonal design, and taking the complexing rate of total flavonoids as assessment criteria, the complex was analyzed by DSC, IR and determined apparent oil-water distribution coefficients in different pH aqueous solution. RESULTS: The optimized preparation conditions for GBE 50-phospholipid complex were obtained as follows: the solvent was Tetrahydrofuran, the temperature was 30 degrees C, the concentration of GBE 50 was 20 mg/mL, the ratio of GBE 50 to phospholipids was 1 to 1, and the complexing rate was 98%. The complex significantly improved GBE 50 on the solubility in octanol, also on the oil-water apparent partition coefficient. CONCLUSION: GBE 50-phospholipid complex is very different from GBE 50 on the physicochemical properties.
