Correlation analysis of the vertebral compression degree and CT HU value in elderly patients with osteoporotic thoracolumbar fractures.

BACKGROUND: To explore the correlation of the vertebral compression degree and cancellous bone CT HU in elderly patients with osteoporotic thoracolumbar fractures. METHODS: Elderly patients with single-segment vertebral fragility fractures were retrospectively reviewed. All patients experienced a low-energy trauma and underwent thoracolumbar MRI. The consistency of measurement between two spine surgeons was evaluated. The average CT HU value of the adjacent vertebral body was used instead. RESULTS: A total of 54 patients were included in the final analysis. The patients' average age was 70.39 ± 8.53 years, and the average CT HU value was 72.78 ± 29.75 HU. The average vertebral compression ratio was 0.57 ± 0.16. Measurements showed both good intrarater repeatability and good interrater reproducibility of the vertebral compression ratio (ICC = 0.978). The degree of vertebral compression in thoracolumbar osteoporotic fractures was strongly positively correlated with the cancellous bone CT HU value (P < 0.01). CONCLUSIONS: The local bone quality as evaluated by the CT HU value is an important factor affecting the degree of compression in osteoporotic vertebral fractures. This study provides quantitative evidence that a greater compression ratio with thoracolumbar osteoporotic fractures was associated with lower bone density in elderly patients. Further longitudinal studies with larger cohorts are needed to verify this relationship.

The Cuproptosis-Related Long Noncoding RNA Signature Predicts Prognosis and Tumour Immune Analysis in Osteosarcoma.

Background: Cuprotopsis is a type of programmed cell death discovered in recent years. Long noncoding RNAs (lncRNAs) play an important regulatory role in programmed cell death. The effect of cuproptosis-related lncRNAs on osteosarcoma is unknown. Our work, based on cuproptosis-related lncRNAs, proposes a gene signature to assess the prognosis of patients with osteosarcoma. Methods: Osteosarcoma gene expression data from The Cancer Genome Atlas (TCGA), clinical features of osteosarcoma and RNA sequencing data of normal adipose tissue were obtained from the UCSC Xena database. A cuproptosis-related lncRNA risk model was established to calculate the risk score. At the same time, cluster analysis, clinicopathological analysis, functional enrichment analysis, and prediction of compounds with potential therapeutic value were evaluated. We analyzed whether there was a correlation between the risk score and tumour immunity. RT-qPCR was used to verify the expression level of lncRNA. Results: Nine lncRNAs (AC124798.1, AC006033.2, AL450344.2, AL512625.2, LINC01060, LINC00837, AC004943.2, AC064836.3, and AC100821.2) were identified to create a risk model and indicate the prognosis of patients with osteosarcoma. The high-risk group had a worse prognosis than the low-risk group. Analysis of clinicopathological features, principal component analysis, receiver operating characteristic curve, c-index curve, and comparative analysis of models proved that the model is reliable. Functional enrichment analysis suggests that the risk score may correlate with cell energy metabolism and tumour-related biological function. Three potentially therapeutic compounds have been predicted. These analyses may be beneficial to the treatment of osteosarcoma in the future. RT-qPCR verified the expression level of three lncRNA (LINC01060, NKILA, and SNHG8). Conclusions: Cuproptosis-related lncRNAs have a strong relationship with osteosarcoma patients. Nine lncRNA models can effectively forecast the prognosis of osteosarcoma and may play a significant role in the individualized treatment of osteosarcoma patients in the future.

Factors associated with the relapse in Ponseti treated congenital clubfoot.

OBJECTIVES: We retrospectively investigated the clinical materials to seek the factors that lead to relapse after using the Ponseti method. METHODS: We retrospectively reviewed all children with congenital clubfoot treated with the Ponseti method in our hospital from June 2008 to June 2013. The data included the following factors: age, gender, initial Pinari score, number of casts, number of feet (unilateral or bilateral), age at the first casting, age of mother, tenotomy, walking age, and compliance with using bracing. All investigations were conducted in conformity with ethical standards. This study was approved by Guilin Peoples' Hospital Ethics Committee. RESULTS: In this study, there were 148 cases (164 ft) in total that underwent the Ponseti method, with the follow-up period at least 5 years. Of them, 64 children presented with left side, 58 with right side, and 26 with bilateral cases. This study included 75 males and 73 females; sex did not affect the outcomes. The mean age of the first casting was 2.50 ± 2.15 months. The average initial Pirani score was 4.98 ± 1.33, and the average number of casts was 5.71 ± 2.28 times. The mean age of mothers at birth was 25.81 ± 2.38 years old. The walking age of children was at a mean of 14.83 ± 1.18 months. Forty-nine cases could not tolerate using braces, namely the rate of noncompliance in this study was 33.1%. Tenotomy was performed on 113 ft (76.4%). The average follow-up period was 7.27 ± 1.29 years (from 5 to 10 years). The rate of relapse was 21.6% (32 cases) at the end of the follow-up. The rate of relapse in the noncompliance with using bracing group was significantly higher compared to the compliance group . CONCLUSION: The initial Pirani score, compliance with the foot abduction brace and the age at the first casting are three independent factors for relapse in clubfoot.