Coordinate action of the helicase and 3' to 5' exonuclease of Werner syndrome protein.

Werner syndrome is a human disorder characterized by premature aging, genomic instability, and abnormal telomere metabolism. The Werner syndrome protein (WRN) is the only known member of the RecQ DNA helicase family that contains a 3' --> 5'-exonuclease. However, it is not known whether both activities coordinate in a biological pathway. Here, we describe DNA structures, forked duplexes containing telomeric repeats, that are substrates for the simultaneous action of both WRN activities. We used these substrates to study the interactions between the WRN helicase and exonuclease on a single DNA molecule. WRN helicase unwinds at the forked end of the substrate, whereas the WRN exonuclease acts at the blunt end. Progression of the WRN exonuclease is inhibited by the action of WRN helicase converting duplex DNA to single strand DNA on forks of various duplex lengths. The WRN helicase and exonuclease act in concert to remove a DNA strand from a long forked duplex that is not completely unwound by the helicase. We analyzed the simultaneous action of WRN activities on the long forked duplex in the presence of the WRN protein partners, replication protein A (RPA), and the Ku70/80 heterodimer. RPA stimulated the WRN helicase, whereas Ku stimulated the WRN exonuclease. In the presence of both RPA and Ku, the WRN helicase activity dominated the exonuclease activity.

Pedestal antiresonant reflecting waveguides for robust coupling to microsphere resonators and for microphotonic circuits.

Strip-line pedestal antiresonant reflecting waveguides are high-confinement, silica integrated optical waveguides in which the optical modes are completely isolated from the substrate by thin high-index layers. These waveguides are particularly well suited for whispering-gallery mode excitation in high-Q microspheres. They can also be used in microphotonic circuits, such as for microring resonators. The theory and design of these structures are highlighted. Experiments that show high coupling efficiency to microspheres are also demonstrated.

Planar integrated wavelength-drop device based on pedestal antiresonant reflecting waveguides and high-Q silica microspheres.

Whispering-gallery modes in silica microspheres can be accessed very efficiently with the recently introduced stripline pedestal antiresonant reflecting optical waveguide (SPARROW) structure. This integrated-optics coupling technique creates novel application opportunities for the high-Q spherical cavities. We report the demonstration of a narrow-band wavelength-drop configuration utilizing SPARROW waveguides and a silica microsphere.

Surface-roughness-induced contradirectional coupling in ring and disk resonators.

Reflections that are due to random surface roughness in periodic structures such as dielectric rings and disks inherently phase match forward- and backward-propagating modes. Small reflections are thus considerably enhanced and may impair the performance of traveling-wave resonators. In addition, such contradirectional coupling leads to a splitting of the resonant peak. These effects are studied analytically.