Assessment of nutritional anemia on the basis of dietary pattern estimation among the population of Garhwal Himalayan region.

BACKGROUND: Nutritional anemia has its prevalence at a global level. Unfortunately, the developing countries are at more risk due to the unavailability of resources and poor socio-economic conditions. According to the WHO, India has >40% prevalence of anemia, which is a severe public health concern. MATERIALS AND METHODS: The aim of this study was to assess the dietary pattern according to their haemoglobin levels. This cross-sectional study was designed to assess the prevalence of anemia in Garhwal population, which contained total 520 individuals (344 females and 176 males). For this a food frequency questionnaire is administered to elicit information on dietary consumption pattern. The frequency of consumption was divided into three levels: ≥ 3 days/wk., 1-2 days/wk. & no or rare consumption. Subjects were divided into 3 groups according to the level of haemoglobin. RESULTS: In the present study, an overall prevalence of anemia was found 34.23% (38.06% in females and 27.61% in males). Results were expressed as Chi-square test and considered significant at 5% level of significance (p< 0.05). CONCLUSION: The overall status of anaemic individuals in the Garhwal region is of vital concern and should be paid due attention for the improvement of nutritional requirements to reduce the prevalence of anemia in this region. The effective measures include awareness about the essential nutrients to be included in diet along with the knowledge of anemia.

Menstrual abnormalities and their association with lifestyle pattern in adolescent girls of Garhwal, India.

BACKGROUND: This study tried to find out the prevalence of menstrual abnormalities in adolescent girls and their association with dietary and exercise pattern in Garhwal region of India. MATERIALS AND METHODS: In this study, cross-sectional questionnaires survey was conducted in different schools of Garhwal. These questionnaires were based on socioeconomic data, dietary and physical activities, and menstrual abnormalities. RESULTS: The age of menarche was 13.5 ± 0.64 years. Dysmenorrhea (62.75%), premenstrual syndrome (PMS) (40.42%), and irregular cycle (28.72%) were observed common problems among adolescent girls. The result showed that dysmenorrhea, PMS, and menstrual irregularities were directly associated with dietary habits and physical activities. Dysmenorrhea was associated with eating junk food (66.10%), and PMS was associated with lacking of physical activities (78.94%). CONCLUSION: The result suggests that feeding habit and physical activities directly affecting the menstrual health of adolescent girls.

N-acetylcysteine effectively mitigates cadmium-induced oxidative damage and cell death in Leydig cells in vitro.

CONTEXT: Cadmium (Cd) is known to cause severe damage to various organs including lung, liver, kidney, brain and reproductive system. Several studies have reported the induction of oxidative stress pathways following Cd exposure. OBJECTIVE: Since oxidative stress is also deemed responsible for inducing male infertility, a growing worldwide concern, we tried to understand whether the antioxidant N-acetylcysteine (NAC) can be a potential therapeutic agent to counter Cd toxicity using primary Leydig cells. MATERIALS AND METHODS: This study highlights the initial cellular alterations which culminate in cell death induction. Primary Leydig cells were isolated from 28-day-old male Wistar rats, exposed to various concentrations of Cd in vitro and biochemical and cell death parameters were evaluated to understand the effect of Cd. NAC pre-treatment was done to understand its protective efficacy. RESULTS: Following Cd exposure to Leydig cells in vitro, we found simultaneous intracellular calcium (Ca(2+)) increase and reduction in mitochondrial membrane polarization at 30 min, followed by significant induction of reactive oxygen species and MAPK-extracellular-regulated kinases with concurrent glutathione depletion at 1 h, and significant cell death (both necrotic and apoptotic) at 6 and 18 h, respectively. Pre-treatment with NAC abrogated all these toxic manifestations and showed significantly reduced cell death. NAC also rescued the expression of 3-ßHSD, a major steroidogenic protein. DISCUSSION AND CONCLUSION: Taken together, these data illustrated that NAC can be used as a potential protective agent against Cd-induced testicular toxicity, especially with regards to oxidative stress-induced Leydig cell toxicity.

Molecular characterization of primary gene pool of chickpea based on ISSR markers.

Genetic diversity and relationships within and among members of the primary gene pool of chickpea, including 38 accessions of Cicer arietinum, six of C. reticulatum,, and four of C. echinospermum, were investigated using 31 ISSR markers. The study revealed moderate diversity, detecting 141 fragments, of which 79 (56%) were polymorphic. Averages were 0.125 for polymorphic information content, 0.350 for marker index, and 0.715 for resolving power. The UPGMA dendrogram and the principal coordinate analysis revealed a clear differentiation between wild and cultivated accessions. The clustering pattern did not strictly follow the grouping of accessions by geographic origin but was in good agreement with the pedigree data and the seed type. The study demonstrates that ISSRs provide promising marker tools in revealing genetic diversity and relationships in chickpea and can contribute to efficient identification, conservation, and utilization of germplasm for plant improvement through conventional as well as molecular breeding approaches.

Interplay of early biochemical manifestations by cadmium insult in sertoli-germ coculture: an in vitro study.

Cadmium is a common environmental and occupational hazard and its adverse effect on reproductive organ has been well documented. The present study is planned to delineate the mechanism of Cd toxicity in rat testes. Our study shows that Cd causes apoptosis in sertoli-germ cells which is governed by oxidative stress. We assayed ROS, GSH and MMP to ensure the role of oxidative stress, further confirmed it by thiol modulators. The initial biochemical response shown in sertoli-germ cells was a significant rise in intracellular calcium followed by a drastic fall in MMP and then ROS generation. The downstream events included cytochrome c release leading to caspase-3 activation and culminating in cell death via apoptosis. Furthermore Cd disrupted the spermatogenic pathway as evident by suppression in tesmin and LDH-X levels.