Production of exopolysaccharides from Bacillus licheniformis, a thermophilic bacteria from hot spring of Jharkhand.

Thermophiles are the microorganisms which thrive under extreme conditions such as high temperature, making them significant for scientific interest. This study provides information based on isolation of thermophilic strain from Surajkund and Ramkund, hot spring of Jharkhand at 50, 60 and 70 °C. Two of the best isolates were used for the extraction of exopolysaccharides. Additionally, the lyophilized product obtained was further analyzed for protein and total sugar estimation. The FTIR analysis revealed the presence of different functional groups such as hydroxyl, C-H stretching, vibration of aliphatic CH2 and glycosidic linkage, thus proving the product obtained from bacteria was an exopolysaccharides The FESEM analysis of exopolysaccharides show varying surface morphology that is from porous to globular structure. Based on 16S rRNA sequences, the isolates from Surajkund (ON795919) and Ramkund (ON795916) were different strains of Bacillus licheniformis. This is the first report on exopolysaccharide secreting thermophilic strain from these hot springs.

Optimisation, characterization, and biological evaluation of novel exopolysaccharide from Bacillus licheniformis (BITSL006).

The study investigated production, characterisation, and biological properties of exopolysaccharide (EPS) from a thermophilic bacterium, Bacillus licheniformis using sucrose as a main carbon source at a temperature of 75 °C, resulting in a yield of 2.87 g/L. The surface topology of EPS was determined using FESEM indicating its porous nature. Subsequently, FTIR was employed to examine EPS and identified the presence of carboxyl and hydroxyl groups, which are believed to be associated with water-holding capacity (WHC). Comparing the FTIR spectrum of various exopolysaccharides, it was inferred that the exopolysaccharide derived from Ramkund closely resembles dextran. EDX and ICP-MS analysis revealed the presence of Sulphur and Selenium which might be involved in the anticancer properties of EPS. This is the first report on bacterial EPS from a hot spring (Ram kund) with antioxidant property, WHC, and high solubility. These properties offer beneficial resources for exploration in the pharmaceutical and agriculture industries.