RESUMO
The experience of a treatment in cooccurring disorders of drug addiction and mental health has allowed to document the intervention with people presenting one or more personality disorders. This article describes the integrated program in which is inserted the trajectory of these people. The article highlights the complexity of their clinical portrait as well as the implications of the most current interventions in their treatment. It also underlines some critical moments of this clientele's path. Clinical vignettes are presented to better illustrate remarks. The authors' reflection leads to reconsidering conditions of the intervention. The avenue proposed is that of a differienciated, intermittent but continuous treatment. The possibility of a long term individual treatment for clients adopting this mode, is also considered. This range of possibilities would better respond to the specific chronicity of these two disorders.
RESUMO
We report the cloning and characterization of a novel member of the Transcriptional Intermediary Factor 1 (TIF1) gene family, human TIF1gamma. Similar to TIF1alpha and TIF1beta, the structure of TIF1beta is characterized by multiple domains: RING finger, B boxes, Coiled coil, PHD/TTC, and bromodomain. Although structurally related to TIF1alpha and TIF1beta, TIF1gamma presents several functional differences. In contrast to TIF1alpha, but like TIF1beta, TIF1 does not interact with nuclear receptors in yeast two-hybrid or GST pull-down assays and does not interfere with retinoic acid response in transfected mammalian cells. Whereas TIF1alpha and TIF1beta were previously found to interact with the KRAB silencing domain of KOX1 and with the HP1alpha, MODI (HP1beta) and MOD2 (HP1gamma) heterochromatinic proteins, suggesting that they may participate in a complex involved in heterochromatin-induced gene repression, TIF1gamma does not interact with either the KRAB domain of KOX1 or the HP1 proteins. Nevertheless, TIF1gamma, like TIF1alpha and TIF1beta, exhibits a strong silencing activity when tethered to a promoter. Since deletion of a novel motif unique to the three TIF1 proteins, called TIF1 signature sequence (TSS), abrogates transcriptional repression by TIF1gamma, this motif likely participates in TIF1 dependent repression.
Assuntos
Família Multigênica , Sequência de Aminoácidos , Sequência de Bases , Homólogo 5 da Proteína Cromobox , Bandeamento Cromossômico , Mapeamento Cromossômico , Clonagem Molecular , Proteínas de Ligação a DNA/genética , Regulação da Expressão Gênica , Humanos , Dados de Sequência Molecular , Proteínas Nucleares/genética , Ligação Proteica , RNA Mensageiro/isolamento & purificação , Proteínas Recombinantes/biossíntese , Proteínas Repressoras/genética , Análise de Sequência de DNA , Homologia de Sequência de Aminoácidos , Distribuição Tecidual , Fatores de Transcrição/genética , Transcrição Gênica , Transfecção , Proteína 28 com Motivo TripartidoRESUMO
The dystrophin gene, which is defective in Duchenne muscular dystrophy (DMD), also encodes a number of smaller products controlled by internal promoters. Dp71, which consists of the two C-terminal domains of dystrophin, is the most abundant product of the gene in non-muscle tissues and is the major product in adult brain. To study the possible function of Dp71 and its expression during development, we specifically inactivated the expression of Dp71 by replacing its first and unique exon and a part of the concomitant intron with a beta-galactosidase reporter gene. X-Gal staining of Dp71-null mouse embryos and tissues revealed a very stage- and cell type-specific activity of the Dp71 promoter during development and during differentiation of various tissues, including the nervous system, eyes, limb buds, lungs, blood vessels, vibrissae and hair follicles. High activity of the Dp71 promoter often seemed to be associated with morphogenic events and terminal differentiation. In some tissues the activity greatly increased towards birth.
