Genomics Analysis of L-DOPA Exposure in Drosophila sechellia.

Drosophila sechellia is a dietary specialist fruit fly that evolved from a generalist ancestor to specialize on the toxic fruit of Morinda citrifolia This species pair has been the subject of numerous studies where the goal has largely been to determine the genetic basis of adaptations associated with host specialization. Because one of the most striking features of M. citrifolia fruit is the production of toxic volatile compounds that kill insects, most genomic studies in D. sechellia to date have focused on gene expression responses to the toxic compounds in its food. In this study, we aim to identify new genes important for host specialization by profiling gene expression response to 3,4-dihydroxyphenylalanine (L-DOPA). Recent work found it to be highly abundant in M. citrifolia, critical for reproductive success of D. sechellia, and supplementation of diet with the downstream pathway product dopamine can influence toxin resistance phenotypes in related species. Here we used a combination of functional genetics and genomics techniques to identify new genes that are important for D. sechellia ecological adaptation to this new niche. We show that L-DOPA exposure can affect toxin resistance phenotypes, identify genes with plastic responses to L-DOPA exposure, and functionally test an identified candidate gene. We found that knock-down of Esterase 6 (Est6) in a heterologous species alters toxin resistance suggesting Est6 may play an important role in D. sechellia host specialization.

Transcriptomic Analysis of Octanoic Acid Response in Drosophila sechellia Using RNA-Sequencing.

The dietary specialist fruit fly Drosophila sechellia has evolved to specialize on the toxic fruit of its host plant Morinda citrifolia Toxicity of Morinda fruit is primarily due to high levels of octanoic acid (OA). Using RNA interference (RNAi), prior work found that knockdown of Osiris family genes Osiris 6 (Osi6), Osi7, and Osi8 led to increased susceptibility to OA in adult D. melanogaster flies, likely representing genes underlying a Quantitative Trait Locus (QTL) for OA resistance in D. sechellia While genes in this major effect locus are beginning to be revealed, prior work has shown at least five regions of the genome contribute to OA resistance. Here, we identify new candidate OA resistance genes by performing differential gene expression analysis using RNA-sequencing (RNA-seq) on control and OA-exposed D. sechellia flies. We found 104 significantly differentially expressed genes with annotated orthologs in D. melanogaster, including six Osiris gene family members, consistent with previous functional studies and gene expression analyses. Gene ontology (GO) term enrichment showed significant enrichment for cuticle development in upregulated genes and significant enrichment of immune and defense responses in downregulated genes, suggesting important aspects of the physiology of D. sechellia that may play a role in OA resistance. In addition, we identified five candidate OA resistance genes that potentially underlie QTL peaks outside of the major effect region, representing promising new candidate genes for future functional studies.

Micronutrient Food Fortification for Residential Care: A Scoping Review of Current Interventions.

PURPOSE: Malnutrition is common in residential care environments, primarily due to poor intake. Micronutrient deficiency, although poorly investigated to date, is also reported to be high. Improving the nutrient density of consumed foods is a potential mechanism to promote increased nutrient intake. A scoping review was conducted to: (1) explore the evidence on micronutrient food fortification strategies, (2) identify candidate nutrients and food vehicles for successful food fortification, and (3) identify gaps for future research. METHODS: The scoping review framework of Arksey and O'Malley was used. A comprehensive search strategy of 4 electronic databases (MEDLINE, EMBASE, CINAHL, and Web of Science) was completed. Two reviewers were involved in screening and data extraction for all selected articles. RESULTS: A total of 4394 relevant articles were identified for screening, and application of inclusion/exclusion criteria resulted in 6 food fortification studies (8 citations; 1 study had 3 citations). Overall, vitamin D (n = 5 studies) and calcium (n = 4 studies) were the most common micronutrients fortified; milk products, margarine, bread, and pureed foods were fortification vehicles. Most studies fortified below the RDA recommendation and did not include clinical outcomes. Samples were small and intervention periods were short (3-6 months). CONCLUSIONS: Fortification is a viable strategy for improving the nutrient density of foods consumed in residential care. Although disparate, this literature suggests the potential for further undertaking of fortification to prevent micronutrient deficiencies among residents and future research should consider multinutrient preparations and clinical outcomes.

Micronutrients on the Menu: Enhancing the Quality of Food in Long-term Care for Regular, Nontherapeutic Menus.

Micronutrient (vitamin and mineral) deficiencies may exacerbate prevalent health conditions occurring in long-term care (LTC) residents, and current food provision may potentiate this problem. A micronutrient-focused, food-first approach to menu planning may address this gap by emphasizing nutrient-dense foods. The objectives were to determine if: (i) selected LTC menus met micronutrient and Canada's Food Guide (CFG) recommendations, and (ii) recommendations can be met through food alone with strategic menu planning. Regular, nontherapeutic menus (week 1, all meals) from diverse LTC homes (n = 5) across Canada were analyzed for micronutrient content using Food Processor and CFG servings. Site dietitians confirmed menu analyses. Five super-menus were created and analyzed for comparison. The nutrient content of the menus varied significantly across homes. Micronutrients of greatest concern were (mean ± SD) vitamin D (8.90 ± 5.29 µg/d) and vitamin E (5.13 ± 1.74 mg/d). Folate, magnesium, and potassium were also below recommendations. Super-menus of equal food volume met recommendations for all micronutrients except vitamin D (56%), vitamin E (84%), and potassium (85%). Meeting most micronutrient recommendations is possible with creative and deliberate menu planning. Knowledge translation of best practices is needed.

Honoring Identity Through Mealtimes in Chinese Canadian Immigrants.

Mealtimes are opportunities for social interactions and expressions of individual and family identity, and serve as a microcosm of the broader lives of families living with dementia. The Eating Together study and its resulting Life Nourishment Theory (LNT) explicated the importance of mealtimes for honouring individual and family identities in the context of dementia. This sub-study examined a specific ethnocultural group with cultural food-ways and caring expectations, to determine if the concept of honouring identity needed to be modified or extended. Using active interview techniques, two Cantonese speaking researchers completed dyad/triad family and individual interviews with six Chinese Canadian immigrant families, recruited from two service providers in a large, urban, multicultural city. This sub-study provided insight into the challenges and rewards of mealtimes for Chinese immigrant families with dementia in the community and specifically provided further insights into the honouring identity concept. Although LNT and specifically the honouring identity concept was generally confirmed in this group, some culturally-specific themes were also identified. This work serves as a basis for future studies examining the meaning and experience of mealtimes in specific cultural groups living with dementia. Such work would confirm if the LNT can be applied to specific ethnocultural groups as well as the general population living with dementia.

A community-based epidemiological study of health anxiety and generalized anxiety disorder.

This community-based study examined the frequency of worry about personal health in respondents with and without generalized anxiety disorder (GAD), and the impact of health anxiety on the disorder. A random community-based telephone survey of 5118 Chinese respondents aged 18-64 was conducted. A fully structured questionnaire covered the DSM-IV-TR criteria of GAD, major depressive episode (MDE), eight domains of worry, the seven-item Whiteley Index (WI-7), health service use, and socio-demographic information. Worry about personal health ranked fifth (75.6%) among eight domains of worries examined. GAD respondents with high level of health anxiety were significantly older, less educated, and had lower family income. High health anxiety significantly increased the occurrence of one-year MDE, previous persistent worry, previous persistent low mood, number of domains of worries, number of non-core DSM-IV-TR GAD symptoms, health service use, and mistrust of doctors. Health anxiety is common in GAD and may signify greater severity of the disorder.

What factors affect influenza vaccine uptake among community-dwelling older Chinese people in Hong Kong general outpatient clinics?

AIM: This study aimed to identify the influenza vaccination rate among community-dwelling older Chinese people in Hong Kong general outpatient clinics and to identify the factors affecting their vaccine uptake. BACKGROUND: Community-dwelling older Chinese people in Hong Kong underuse influenza vaccination. The first step to increase their vaccination rates is to understand the factors affecting their influenza vaccine uptake. DESIGN: This was a retrospective and descriptive study. METHODS: The sample comprised 197 subjects from five general outpatient clinics in the Kowloon East cluster of Hong Kong. A self-report questionnaire was used for data collection via individual face-to-face interviews. RESULTS: Out of 197 subjects, 64.47% reported having received the vaccine within the previous 12 months. The regression model revealed that perceived benefits including 'vaccination prevents me from catching influenza (OR = 12.18, CI = 3.61-41.07, p < 0.001)', 'If I get vaccinated, I will decrease the frequency of medical consultation (OR = 8.12, CI = 2.70-24.38, p = 0.001)' and 'If I am vaccinated and still get flu, I will not be as sick with it (OR = 0.43, CI = 0.24-0.76, p = 0.004)', perceived barriers, which are 'the side-effects of influenza vaccination interfere with my usual activities (OR = 0.04, CI = 0.01-0.13, p < 0.001)', 'influenza vaccination is painful (OR = 2.73, CI = 1.55-4.81, p = 0.001)' and 'I am scared of needles (OR = 0.43, CI = 0.23-0.79, p = 0.007)' and recommendations from doctors (OR = 14.18, CR = 4.09-49.16, p < 0.001) and families (OR = 3.67, CR = 1.24-10.83, p = 0.019) interactively influenced the subjects' vaccine uptake. CONCLUSION: Specific benefits and barriers affecting the influenza uptake of community-dwelling older Chinese people in general outpatient clinics have been identified. Specifically, recommendation from families plays a significant role in motivating the uptake of this Chinese ageing group. RELEVANCE TO CLINICAL PRACTICE: We adopt strategies including personal invitation and reminders of influenza vaccination by doctors and educating doctors, older people and their families on influenza vaccination to increase the uptake of community-dwelling Chinese older people in general outpatient clinics.

Chinese older people in Hong Kong: health beliefs about influenza vaccination.

A health belief survey of 70 community-dwelling Chinese older people was carried out in five general outpatient clinics in the Kowloon East hospital cluster of Hong Kong. Subjects were asked about their susceptibility to influenza, their perceptions of its severity, and benefits of and barriers to vaccination. Beliefs in their own good health, the effectiveness of cultural measures adopted to prevent influenza and its curability may be among the reasons why those who decline the vaccination thought they were unlikely to catch the virus. Community nurses should help modify people's health beliefs to motivate them to take up vaccination.

Bone mineral density differences between adolescent dancers and non-exercising adolescent females.

STUDY OBJECTIVE: To compare the bone mineral density (BMD) of the axial and appendicular skeleton between regularly exercising collegiate dancers and age matched non-exercising young females between the age of 17 and 19 to assess the impact of weight-bearing exercises and menstrual status on BMD. DESIGN: Prospective observational cohort. SETTING: Sports clinic in a collegiate school of dance and a hospital-based adolescent clinic. PARTICIPANTS: The adolescent dancers consisted of full-time collegiate dance students from a tertiary Performing Arts Institute (n = 35). The non-exercising controls consisted of eumenorrhoeic patients of the same age presenting to the Adolescent Clinic (n = 35). INTERVENTIONS: All subjects had a full hormonal profile, bio-impedance estimation of body fat, and dual energy X-ray absorptiometry and quantitative peripheral CT scans (pQCT) to determine bone density. MAIN OUTCOME MEASURES: Comparison of the mean bone mineral density in the axial and appendicular skeleton between the two groups. RESULTS: The incidence of oligo/amenorrhoea in the dancers was 20%. The lumbar spine BMD (1.006 g/cm(2) vs. 0.938, P = 0.048) and hip BMD (neck of femur 0.978 g/cm(2) vs. 0.838, P < 0.001; Ward's triangle 0.816 g/cm(2) vs. 0.720, P = 0.003; trochanter 0.777 g/cm(2) vs. 0.682; P < 0.001) were significantly higher in the eumenorrhoeic dancers as compared to controls. The radial BMD as measured by pQCT did not differ between the two groups, but the core trabecular tibial BMD was also higher in the dancers (321 mg/cm(3) vs. 286, P = 0.006). When only oligo/amenorrhoeic dancers (n = 7) were compared with the controls, the same differences in BMD values were no longer observed. CONCLUSION: Young women undergoing regular intensive weight-bearing exercises as in the collegiate dancers here studied have higher BMD in the axial and appendicular skeleton as compared to non-exercising females of the same age if they remain eumenorrhoeic during their training. This advantage was apparently lost when they developed oligo/amenorrhoea.