Evaluation of carbon sources from sugar industry to bacterial nanocellulose produced by Komagataeibacter xylinus.

Nanocellulose derived from microorganism is crucial bio-based products due to its unique physicochemical and mechanical properties for material science. Thus, optimizing bacterial cellulose (BNC) production is essential to widen applications and reduce production cost. Using various carbon sources derive from fruits as alternatives for synthesizing BNC could produce a low-cost BNC with comparable properties. Although Komagataeibacter xylinus grown in different natural juices, including clarified juice (CJ), sugarcane juice (SC) and coconut juice (CN) demonstrated a lower yield than that of control medium (HS), FTIR confirmed no change in chemical functional groups of BNCs. Similarly, different sugar sources have slightly effects on mechanical and thermal properties of BNC. However, the internal morphology illustrated the pore structure in oval shape for HS and CN while CJ and SC resulted in irregular pores which could lead to the highest crystallinity index value for BNC from HS compared to that from alternative media.

Production of hydroxyapatite-bacterial nanocellulose scaffold with assist of cellulose nanocrystals.

Bacterial nanocellulose/hydroxyapatite/cellulose nanocrystal (BHC) composites were synthesized via in-situ synthesis using cellulose nanocrystals (CNCs) to improve colloidal stability and the dispersion of hydroxyapatite (HA) during the bacterial nanocellulose (BNC) cultivation period. Transmission electron microscopy images and energy dispersive spectroscopy (EDS) results confirmed the dispersion of HA on the CNC particles with a Ca/P ratio of 1.66 corresponding to that of the stoichiometric HA. The SEM images and EDS results showed that the integration of the HA and BNC network without CNC assistance (BHA (0.25 and 0.5 wt.%) composites) was less than that for BHC at both concentrations. Fourier-transform infrared analysis, XRD and thermal degradation revealed the effect of HA on the BHC composites with a decreased CrI% and improved thermal property. Cytotoxicity proved the potential for using BHC composites for bone tissue engineering scaffold with cell viability up to 83.4 ± 3.6% compared to the negative control (99.2 ± 0.08%).

Homogenous isolation of individualized bacterial nanofibrillated cellulose by high pressure homogenization.

Varying levels of high pressure homogenization (HPH) were applied to disintegrate bacterial nanofibrillated cellulose (BNFC) from bacterial cellulose (BC). HPH was considered as a simple, non-toxic and highly efficient physical method for nanofibrillated cellulose extraction. The blended BC passed through chambers at high pressures of 68, 138 and 207MPa for 30 cycles. The particle size confirmed disintegration of the BC network fibers to bundles of BNFC and the atomic force microscopy images showed the decreased diameter of individual BNFC in the range 36-67nm. Fourier transform infrared spectroscopy measurement indicated there were no change in the chemical functional groups of the BNFC compared with BC. The decreased crystallinity index and crystallite size of BNFC with increased pressure confirmed the effect of HPH on the BNFC. Nevertheless, BNFC at 207MPa had the lowest thermal stability due to having the highest surface area, which resulted in the minimum nanofiber diameter.