Assuntos
Técnicas de Química Analítica/instrumentação , Descoberta de Drogas/instrumentação , Indústria Farmacêutica/instrumentação , Indústria Farmacêutica/tendências , Automação Laboratorial/economia , Educação em Farmácia/tendências , Processamento Eletrônico de Dados/economia , Humanos , Colaboração Intersetorial , Laboratórios/tendências , Pessoal de Laboratório/educação , Serviços TerceirizadosRESUMO
Distribution of drugs into tissues is an important determinant of the overall PK and PD profile. Thus, bioanalysis of drugs and their metabolites in tissues can play an important role in understanding the pharmacological and toxicological properties of new drug candidates. Unlike liquid matrices, bioanalysis in tissues offers unique challenges such as proper tissue sampling, appropriate tissue sample preparation, efficient extraction of the analytes from the tissue homogenates, and demonstration of stability and recovery of analytes in intact tissues. This article provides a systematic review of tissue sample analysis for small molecules using LC-MS/MS. The authors provide rationale for tissue sample analysis, and discuss strategies for method development, method qualification or validation, and sample analysis. Unique aspects of method development and qualification/validation are highlighted based on authors' direct experiences and literature summary. Analysis using intact tissue samples such as MALDI imaging is also briefly discussed.
