RESUMO
BACKGROUND AND PURPOSE: A rapid identification of the etiology of anterior ischemic optic neuropathy is crucial because it determines therapeutic management. Our aim was to assess MR imaging to study the optic nerve head in patients referred with anterior ischemic optic neuropathy, due to either giant cell arteritis or the nonarteritic form of the disease, compared with healthy subjects. MATERIALS AND METHODS: Fifteen patients with giant cell arteritis-related anterior ischemic optic neuropathy and 15 patients with nonarteritic anterior ischemic optic neuropathy from 2 medical centers were prospectively included in our study between August 2015 and May 2016. Fifteen healthy subjects and patients had undergone contrast-enhanced, flow-compensated, 3D T1-weighted MR imaging. The bright spot sign was defined as optic nerve head enhancement with a 3-grade ranking system. Two radiologists and 1 ophthalmologist independently performed blinded evaluations of MR imaging sequences with this scale. Statistical analysis included interobserver agreement. RESULTS: MR imaging scores were significantly higher in patients with giant cell arteritis-related anterior ischemic optic neuropathy than in patients with nonarteritic anterior ischemic optic neuropathy (P ≤ .05). All patients with giant cell arteritis-related anterior ischemic optic neuropathy (15/15) and 7/15 patients with nonarteritic anterior ischemic optic neuropathy presented with the bright spot sign. No healthy subjects exhibited enhancement of the anterior part of the optic nerve. There was a significant relationship between the side of the bright spot and the side of the anterior ischemic optic neuropathy (P ≤ .001). Interreader agreement was good for observers (κ = 0.815). CONCLUSIONS: Here, we provide evidence of a new MR imaging sign that identifies the acute stage of giant cell arteritis-related anterior ischemic optic neuropathy; patients without this central bright spot sign always had a nonarteritic pathophysiology and therefore did not require emergency corticosteroid therapy.
Assuntos
Arterite de Células Gigantes/complicações , Arterite de Células Gigantes/diagnóstico por imagem , Imageamento por Ressonância Magnética/métodos , Neuropatia Óptica Isquêmica/diagnóstico por imagem , Neuropatia Óptica Isquêmica/etiologia , Idoso , Idoso de 80 Anos ou mais , Diagnóstico Precoce , Feminino , Humanos , Processamento de Imagem Assistida por Computador , Imageamento Tridimensional , Masculino , Pessoa de Meia-Idade , Variações Dependentes do Observador , Disco Óptico/diagnóstico por imagem , Estudos Prospectivos , Reprodutibilidade dos Testes , Sensibilidade e EspecificidadeRESUMO
This fMRI study aimed to explore the effect of normal aging on word retrieval and generation. The question addressed is whether lexical production decline is determined by a direct mechanism, which concerns the language operations or is rather indirectly induced by a decline of executive functions. Indeed, the main hypothesis was that normal aging does not induce loss of lexical knowledge, but there is only a general slowdown in retrieval mechanisms involved in lexical processing, due to possible decline of the executive functions. We used three tasks (verbal fluency, object naming, and semantic categorization). Two groups of participants were tested (Young, Y and Aged, A), without cognitive and psychiatric impairment and showing similar levels of vocabulary. Neuropsychological testing revealed that older participants had lower executive function scores, longer processing speeds, and tended to have lower verbal fluency scores. Additionally, older participants showed higher scores for verbal automatisms and overlearned information. In terms of behavioral data, older participants performed as accurate as younger adults, but they were significantly slower for the semantic categorization and were less fluent for verbal fluency task. Functional MRI analyses suggested that older adults did not simply activate fewer brain regions involved in word production, but they actually showed an atypical pattern of activation. Significant correlations between the BOLD (Blood Oxygen Level Dependent) signal of aging-related (A > Y) regions and cognitive scores suggested that this atypical pattern of the activation may reveal several compensatory mechanisms (a) to overcome the slowdown in retrieval, due to the decline of executive functions and processing speed and (b) to inhibit verbal automatic processes. The BOLD signal measured in some other aging-dependent regions did not correlate with the behavioral and neuropsychological scores, and the overactivation of these uncorrelated regions would simply reveal dedifferentiation that occurs with aging. Altogether, our results suggest that normal aging is associated with a more difficult access to lexico-semantic operations and representations by a slowdown in executive functions, without any conceptual loss.
Assuntos
Envelhecimento/fisiologia , Encéfalo/fisiologia , Função Executiva/fisiologia , Imageamento por Ressonância Magnética/métodos , Rememoração Mental/fisiologia , Semântica , Vocabulário , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Processamento de Imagem Assistida por Computador , Masculino , Pessoa de Meia-Idade , Testes Neuropsicológicos , Valores de ReferênciaRESUMO
OBJECTIVES: Recurrent peripheral vestibulopathy (RPV) is a public health problem, yet the aetiology remains unclear. Recent developments in MRI of endolymphatic hydrops (EH) allow for a better understanding of inner ear disorders. We intended to study the prevalence of EH in patients with RPV, in comparison to those with Meniere's disease (MD). METHODS: MRI examinations were performed 4 hours after intravenous injection of gadoteric acid in 132 patients with RPV (n = 64) and MD (n = 68). Two radiologists retrospectively studied the prevalence and localization of EH in RPV and MD groups. Patients were graded based on the number and localization of hydrops, between 1 (EH in either cochlea or vestibule on one side) and 4 (EH in cochlea and vestibule on both ears). RESULTS: We identified EH in 31 out of 64 patients and in 61 out of 68 patients in the RPV and MD groups, respectively. There was a significant difference regarding the number of subjects with EH between the two groups (p ≤ 0.01), with a higher average number of hydrops localization in MD group (p ≤ 0.01). CONCLUSION: MRI may reveal EH in some cases among patients with RPV, suggesting a similar pathophysiological mechanism in comparison with MD. KEY POINTS: ⢠MRI may reveal endolymphatic hydrops in some patients with recurrent peripheral vestibulopathy. ⢠We suggest a similar pathophysiological mechanism in recurrent vestibulopathy and Meniere's Disease. ⢠MRI with delayed acquisition helps clinicians to assess patients with recurrent vestibulopathy. ⢠The outcome would be to aid the development of adapted therapeutic strategies. ⢠MRI of endolymphatic hydrops should probably be included in future diagnostic protocols.
Assuntos
Hidropisia Endolinfática/diagnóstico , Doenças Vestibulares/diagnóstico , Cóclea , Meios de Contraste , Diagnóstico Diferencial , Feminino , Humanos , Injeções Intravenosas , Imageamento por Ressonância Magnética/métodos , Masculino , Doença de Meniere/diagnóstico , Pessoa de Meia-Idade , Variações Dependentes do Observador , Exame Físico , Recidiva , Estudos RetrospectivosRESUMO
The potential ergogenic effects of oral salbutamol intake were demonstrated for decades but the underlying mechanisms remain to elucidate. We hypothesized that improved exercise performance after acute oral salbutamol administration is associated with changes in muscle metabolism. Twelve healthy, nonasthmatic, moderately trained, male subjects were recruited to compare in a double-blind crossover randomized study, an oral dose of salbutamol (4 mg) and a placebo. After treatment administration, subjects performed repetitive plantar flexions to exhaustion in a 3T magnet. Continuous (31) P nuclear magnetic resonance spectroscopy assessment of the calf muscles was performed at rest, during exercise, and during recovery. No significant difference between treatments was detected in metabolite concentration at rest (P > 0.05). Creatine phosphate and inorganic phosphate changes during and immediately after exercise were similar between treatments (P > 0.05). Intramuscular pH (pHi) was significantly higher at rest, at submaximal exercise but not at exhaustion with salbutamol (pHi at 50% of exercise duration, 6.8 ± 0.1/6.9 ± 0.1 for placebo and salbutamol, respectively, P < 0.05). The maximal power (28 ± 7 W/23 ± 7 W; P = 0.001) and total work (1702 ± 442 J/1381 ± 432 J; P = 0.003) performed during plantar flexions were significantly increased with salbutamol. Salbutamol induced significant improvement in calf muscle endurance with similar metabolic responses during exercise, except slight differences in pHi. Other mechanisms than changes in muscle metabolism may be responsible for the ergogenic effect of salbutamol administration.
Assuntos
Agonistas de Receptores Adrenérgicos beta 2/farmacologia , Albuterol/farmacologia , Fadiga Muscular/efeitos dos fármacos , Músculo Esquelético/efeitos dos fármacos , Fosfatos/metabolismo , Fosfocreatina/efeitos dos fármacos , Adulto , Estudos Cross-Over , Método Duplo-Cego , Humanos , Concentração de Íons de Hidrogênio , Perna (Membro) , Espectroscopia de Ressonância Magnética , Masculino , Músculo Esquelético/metabolismo , Fosfocreatina/metabolismo , Isótopos de Fósforo , Resistência Física/efeitos dos fármacos , Adulto JovemRESUMO
The functional imaging of perfusion enables the study of its properties such as the vasoreactivity to circulating gases, the autoregulation and the neurovascular coupling. Downstream from arterial stenosis, this imaging can estimate the vascular reserve and the risk of ischemia in order to adapt the therapeutic strategy. This method reveals the hemodynamic disorders in patients suffering from Alzheimer's disease or with arteriovenous malformations revealed by epilepsy. Functional MRI of the vasoreactivity also helps to better interpret the functional MRI activation in practice and in clinical research.
Assuntos
Encéfalo/irrigação sanguínea , Circulação Cerebrovascular , Imageamento por Ressonância Magnética , Transtornos Cerebrovasculares/diagnóstico , HumanosRESUMO
High-field 3 T magnetic resonance imaging (MRI) has entered standard clinical practice over the past decade, and its advantages have already been suggested in areas such as neural, musculoskeletal, pelvic and angiographic imaging. However, high-field systems still pose challenges in terms of their specific absorption rate (SAR) and radiofrequency (RF) excitation uniformity. Thus, the aim of the present study was to evaluate the impact, on both these factors, of standard quadrature against parallel RF transmission technology (dual-source parallel RF excitation [DSPE]) in spinal examination at 3 T. The thoracolumbar spine was examined with three different sequences: T1-weighted (T1w); T2-weighted (T2w); and T2w short tau inversion recovery (STIR). Each was acquired with and without DSPE. The manufacturer's implementation of this technology has been associated with optimized handling of patient SAR exposure, resulting in a 38.4% reduction in acquisition time. On comparing sequences with equal repetition times (TRs), the acquisition time reduction was 44.4%. Thus, DSPE allows a reduction in acquisition time. This gain is accompanied by augmentation of the whole-body SAR and diminution of the local SAR. Image quality improvement due to more homogeneous effective transmit B1 was mainly observed at the junction of the thoracolumbar spine.
Assuntos
Artefatos , Aumento da Imagem/métodos , Vértebras Lombares/anatomia & histologia , Imageamento por Ressonância Magnética/métodos , Medula Espinal/anatomia & histologia , Vértebras Torácicas/anatomia & histologia , Imagem Corporal Total/métodos , Adulto , Algoritmos , Feminino , Humanos , Interpretação de Imagem Assistida por Computador/métodos , Masculino , Pessoa de Meia-Idade , Doses de Radiação , Reprodutibilidade dos Testes , Sensibilidade e EspecificidadeRESUMO
PURPOSE: Pilocytic astrocytomas (PA) and hemangioblastomas (HB) can present the same morphological characteristics on conventional MRI sequences, most usually in the form of a cerebellar cystic mass with a mural nodule that strongly enhances on post-contrast T1 images. We discuss here the value of perfusion MRI in the differentiation of these two tumors, the diagnoses of which have already been histopathologically established. METHOD: Eleven patients with PA and eight with HB underwent first-pass perfusion MRI. The maximum relative cerebral blood volume (rCBV(max)), defined as the ratio between the CBV(max) in tumor tissue and the CBV in healthy, contralateral white matter, is considered to be indicative of the type of tumor. RESULTS: The difference between the rCBV(max) of PA (rCBV(max)=1.19+/-0.71, range 0.6-3.27) compared with that of HB (rCBV(max)=9.37+/-2.37, range 5.38-13) was significant (P<0.001). The first-pass curve crossed the baseline, corresponding to vascular permeability problems in both PA and HB. CONCLUSION: The first-pass method of perfusion MRI is a quick and useful way to differentiate between PA and HB.
Assuntos
Astrocitoma/diagnóstico , Volume Sanguíneo , Neoplasias Encefálicas/diagnóstico , Encéfalo/irrigação sanguínea , Circulação Cerebrovascular , Hemangioblastoma/diagnóstico , Imageamento por Ressonância Magnética/métodos , Adolescente , Adulto , Idoso , Astrocitoma/irrigação sanguínea , Encéfalo/patologia , Neoplasias Encefálicas/irrigação sanguínea , Criança , Diagnóstico Diferencial , Feminino , Hemangioblastoma/irrigação sanguínea , Humanos , Masculino , Pessoa de Meia-Idade , Adulto JovemRESUMO
The matrix-inserted surface transplantation model is an in vivo assay used to analyse the kinetics of tumor-vessel interactions during different stages of skin carcinoma progression. This system allows the study of host-tumor interface, i.e. penetration of tumor cells into normal host tissue as well as infiltration of normal host cells into the tumor. In the present study, image analysis algorithms for processing and quantifying the extent of such migratory and tissue remodeling events are presented. The proposed method is non-parametric and its originality lies in its particularity to take into account the specific geometry of tumor-host interface. This methodology is validated by evaluating the contribution of matrix metalloproteases (MMPs) in skin carcinoma invasion and vascularization through pharmacological and genetic approaches.
Assuntos
Neovascularização Patológica/patologia , Processamento de Sinais Assistido por Computador , Neoplasias Cutâneas/irrigação sanguínea , Neoplasias Cutâneas/patologia , Algoritmos , Inibidores da Angiogênese/farmacologia , Inibidores da Angiogênese/uso terapêutico , Animais , Linhagem Celular Tumoral , Movimento Celular , Dipeptídeos/farmacologia , Dipeptídeos/uso terapêutico , Cinética , Metaloproteinases da Matriz Secretadas/antagonistas & inibidores , Metaloproteinases da Matriz Secretadas/genética , Metaloproteinases da Matriz Secretadas/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Modelos Estatísticos , Invasividade Neoplásica , Neovascularização Patológica/enzimologia , Neovascularização Patológica/prevenção & controle , Inibidores de Proteases/farmacologia , Inibidores de Proteases/uso terapêutico , Reprodutibilidade dos Testes , Neoplasias Cutâneas/enzimologia , Neoplasias Cutâneas/prevenção & controle , Células Estromais/patologiaRESUMO
Synchrotron stereotactic radiotherapy (SSR) is a radiotherapy technique that makes use of the interactions of monochromatic low energy x-rays with high atomic number (Z) elements. An important dose-enhancement can be obtained if the target volume has been loaded with a sufficient amount of a high-Z element, such as iodine. In this study, we compare experimental dose measurements, obtained with normoxic polymer gel (nPAG), with Monte Carlo computations. Gels were irradiated within an anthropomorphic head phantom and were read out by magnetic resonance imaging. The dose-enhancement due to the presence of iodine in the gel (iodine concentration: 5 and 10 mg ml(-1)) was measured at two radiation energies (35 and 80 keV) and was compared to the calculated factors. nPAG dosimetry was shown to be efficient for measuring the sharp dose gradients produced by SSR. The agreement between 3D gel dosimetry and calculated dose distributions was found to be within 4% of the dose difference criterion and a distance to agreement of 2.1 mm for 80% of the voxels. Polymer gel doped with iodine exhibited higher sensitivity, in good agreement with the calculated iodine-dose enhancement. We demonstrate in this preliminary study that iodine-doped nPAG could be used for measuring in situ dose distributions for iodine-enhanced SSR treatment.
Assuntos
Géis/efeitos da radiação , Iodo/efeitos da radiação , Polímeros/efeitos da radiação , Radiometria/métodos , Radiocirurgia/métodos , Relação Dose-Resposta à Radiação , Iodo/química , Dosagem Radioterapêutica , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , SíncrotronsRESUMO
In the last decade, functional MRI (fMRI) has become one of the most widely used functional imaging technique in neurosciences. However, its clinical applications remain limited. Despite methodological and practical issues, fMRI data has been validated by different techniques (magnetoencephalography, Wada test, electrical and magnetic stimulations, and surgical resections). In neurosurgical practice, fMRI can identify eloquent areas involved in motor and language functions, and may evaluate characteristics of postoperative neurological deficit including its occurrence, clinical presentation and duration. This may help to inform patients and to prepare postoperative care. fMRI may also identify epileptic foci. In neurological practice, fMRI may help to determine prognosis of recovery after stroke, appropriate medication, and rehabilitation. fMRI may help to identify patients at risk of developing Alzheimer disease. Finally, cerebrovascular reactivity imaging is an interesting approach that might provide new radiological insights of vascular function.
Assuntos
Encefalopatias/diagnóstico , Imageamento por Ressonância Magnética , Encéfalo/metabolismo , Encefalopatias/metabolismo , Humanos , Imageamento por Ressonância Magnética/métodos , Oxigênio/sangueRESUMO
Vessel size index (VSI) measurements have been validated on rats bearing a glioma with high doses of contrast agent. The aim of this study was to evaluate the impact of using a reduced dose of contrast agent, necessary for clinical trials. Experiments were performed on rats at three doses of AMI-227 and clearly show higher VSI values at lower doses, whatever the region of interest (contralateral, peritumoral and intratumoral tissue). These results are in good correlation with Monte-Carlo simulations on healthy brain and suggest that only relative values can be obtained at clinical contrast agent doses and magnetic fields.
Assuntos
Neoplasias Encefálicas/irrigação sanguínea , Neoplasias Encefálicas/patologia , Ferro/administração & dosagem , Imageamento por Ressonância Magnética/métodos , Microcirculação/patologia , Neovascularização Patológica/patologia , Óxidos/administração & dosagem , Animais , Linhagem Celular Tumoral , Meios de Contraste/administração & dosagem , Corpo Estriado/irrigação sanguínea , Corpo Estriado/patologia , Dextranos , Relação Dose-Resposta a Droga , Óxido Ferroso-Férrico , Glioma/irrigação sanguínea , Glioma/patologia , Aumento da Imagem/métodos , Nanopartículas de Magnetita , Masculino , Ratos , Ratos Wistar , Reprodutibilidade dos Testes , Sensibilidade e EspecificidadeRESUMO
Vessel size imaging (VSI) for brain tumor characterization was evaluated and the vessel size index measured by MRI (VSIMRI) was correlated with VSI obtained by histology (VSIhisto). Blood volume (BV) and VSI maps were obtained on 12 rats by simultaneous measurements of R2* and R2, before and after the injection of a macromolecular contrast agent, AMI-227. Immunostaining of collagen IV in vessels was performed. An expression was derived for evaluating VSI from stereologic measurements on histology data (VSIhisto). On BV and VSI images obtained from large-size tumors (n = 9), three regions could be distinguished and correlated well with histological sections: a high BV region surrounding the tumor, a necrotic area where BV is very low, and a viable tumor tissue region showing lower BV but higher VSI than the normal rat cortex, with the presence of larger vessels. The quantitative analysis showed a good correlation (Spearman rank's rho = 0.74) between VSIhisto and VSIMRI with a linear regression coefficient of 1.17. The good correlation coefficient supports VSI imaging as a quantitative method for tumor vasculature characterization.
Assuntos
Neoplasias Encefálicas/irrigação sanguínea , Glioma/irrigação sanguínea , Imageamento por Ressonância Magnética , Neovascularização Patológica/patologia , Animais , Volume Sanguíneo/fisiologia , Neoplasias Encefálicas/patologia , Colágeno Tipo IV/análise , Meios de Contraste , Dextranos , Modelos Animais de Doenças , Óxido Ferroso-Férrico , Glioma/patologia , Aumento da Imagem , Processamento de Imagem Assistida por Computador , Imuno-Histoquímica , Ferro , Modelos Lineares , Substâncias Macromoleculares , Nanopartículas de Magnetita , Masculino , Microcirculação/patologia , Necrose , Óxidos , Ratos , Ratos Wistar , Estatísticas não ParamétricasRESUMO
Hyperpolarized (129)Xe dissolved in a lipid emulsion constitutes an NMR tracer that can be injected into the blood stream, enabling blood-flow measurement and perfusion imaging. A small volume (0.15 ml) of this tracer was injected in 1.5 s in rat carotid and (129)Xe MR spectra and images were acquired at 2.35 T to evaluate the potential of this approach for cerebral studies. Xenon spectra consistently showed two resonances, at 194.5 ppm and 199.0 ppm relative to the gas peak. The signal-to-noise ratio (SNR) obtained for the two peaks was sufficient (ranging from 12 to 90) to follow their time courses. 2D transverse-projection xenon images were obtained with an in-plane resolution of 900 microm per pixel (SNR range 8-15). Histological analysis revealed no brain damage except in two rats that had received three injections.
Assuntos
Encéfalo/irrigação sanguínea , Espectroscopia de Ressonância Magnética/métodos , Isótopos de Xenônio/farmacocinética , Animais , Emulsões , Injeções Intravenosas , Lipídeos/administração & dosagem , Lipídeos/farmacocinética , Ratos , Processamento de Sinais Assistido por Computador , Isótopos de Xenônio/administração & dosagemRESUMO
The purpose of this study was to evaluate the recently proposed technique of 3He prepolarization at low temperature and high field (Kober et al. Magn Reson Med 1999; 41:1084-1087) for fast imaging of the lung. Helium-3 was cooled to 2.4 K in a magnetic field of 8 Tesla to obtain a polarization of 0.26%. The polarized 3He was warmed up to room temperature and transferred to a rat, with a final polarization of about 0.1%, large enough for acquiring a 3D image of the rat lung in 30 s.
Assuntos
Hélio , Aumento da Imagem , Processamento de Imagem Assistida por Computador , Imageamento Tridimensional , Pulmão/anatomia & histologia , Imageamento por Ressonância Magnética , Animais , Temperatura Baixa , Isótopos , RatosRESUMO
Numerous techniques have been proposed in the last 15 years to measure various perfusion-related parameters in the brain. In particular, two approaches have proven extremely successful: injection of paramagnetic contrast agents for measuring cerebral blood volumes (CBV) and arterial spin labeling (ASL) for measuring cerebral blood flows (CBF). This review presents the methodology of the different magnetic resonance imaging (MRI) techniques in use for CBV and CBF measurements and briefly discusses their limitations and potentials.
Assuntos
Circulação Cerebrovascular , Imageamento por Ressonância Magnética/métodos , Animais , Meios de Contraste , Humanos , Modelos Teóricos , Marcadores de SpinRESUMO
Vessel size imaging is a new method that is based on simultaneous measurement of the changes Delta R(2) and Delta R(2)(*) in relaxation rate constants induced by the injection of an intravascular superparamagnetic contrast agent. Using the static dephasing approximation for Delta R(2)(*) estimation and the slow-diffusion approximation for Delta R(2) estimation, it is shown that the ratio Delta R(2)/Delta R(2)(*) can be expressed as a function of the susceptibility difference between vessels and brain tissue, the brain water diffusion coefficient, and a weighted mean of vessel sizes. Comparison of the results with 1) the Monte Carlo simulations used to quantify the relationship between tissue parameters and susceptibility contrast, 2) the experimental MRI data in the normal rat brain, and 3) the histologic data establishes the validity of this approach. This technique, which allows images of a weighted mean of the vessel size to be obtained, could be useful for in vivo studies of tumor vascularization. Magn Reson Med 45:397-408, 2001.
Assuntos
Encéfalo/irrigação sanguínea , Aumento da Imagem , Processamento de Imagem Assistida por Computador , Angiografia por Ressonância Magnética , Animais , Artérias Cerebrais/patologia , Veias Cerebrais/patologia , Simulação por Computador , Meios de Contraste , Dextranos , Feminino , Óxido Ferroso-Férrico , Ferro , Nanopartículas de Magnetita , Masculino , Microcirculação/patologia , Óxidos , Consumo de Oxigênio/fisiologia , Ratos , Ratos Endogâmicos , Ratos Sprague-Dawley , Sensibilidade e Especificidade , Resistência Vascular/fisiologiaRESUMO
Hyperpolarized 129Xe was dissolved in a lipid emulsion and administered to anaesthetized rats by manual injections into the carotid (approximately 1-1.5 mL in a maximum time of 30 s). During injection, 129Xe NMR brain spectra at 2.35 T were recorded over 51 s, with a repetition time of 253 ms. Two peaks assigned to dissolved 129Xe were observed (the larger at 194 +/- 1 ppm assigned to intravascular xenon and the smaller at 199 +/- 1 ppm to xenon dissolved in the brain tissue). Their kinetics revealed a rapid intensity increase, followed by a plateau (approximately 15 s duration) and then a decrease over 5 s. This behaviour was attributed to combined influences of the T1 relaxation of the tracer, of radiofrequency sampling, and of the tracer perfusion rate in rat brain. Similar kinetics were observed in experiments carried out on a simple micro-vessel phantom. An identical experimental set-up was used to acquire a series of 2D projection 129Xe images on the phantom and the rat brain.
Assuntos
Encéfalo/anatomia & histologia , Espectroscopia de Ressonância Magnética , Animais , Emulsões , Emulsões Gordurosas Intravenosas , Injeções Intra-Arteriais , Masculino , Ratos , Ratos Sprague-Dawley , Isótopos de XenônioRESUMO
This work reports a low-cost and versatile electronic device designed to trigger NMR acquisitions from the cardiac cycle of an isolated perfused heart, or to perform electrical stimulation of the heart. The triggering is synchronised with the pressure curve of the perfused heart. The cardiac pacing is achieved from pulses of the NMR system, or by an internal pulse generator, in order to be operated separately from the NMR instrument.
Assuntos
Estimulação Cardíaca Artificial/métodos , Coração/anatomia & histologia , Coração/fisiologia , Animais , Estimulação Elétrica , Técnicas In Vitro , Espectroscopia de Ressonância Magnética/métodos , Marca-Passo Artificial , Perfusão , CoelhosRESUMO
In this work, two distinct approaches to the tailoring of selective radiofrequency (RF) pulses were applied to a cos-sinc pulse of the DIGGER sequence used in localized spectroscopy. Ideally, three such pulses combined with a gradient in each direction destroy the Mz component of the magnetization everywhere but in the volume of interest. In the first approach, the optimal RF amplitude modulation is found by minimizing the difference between the ideal and the computed Mz profiles. In the second strategy, the RF pulse envelope is adjusted by optimization of the tip angle profile. Computed slice profiles optimized by each of these methods have been compared to experimental results. Simulations as well as experiments show that the second approach, which optimizes tip angles, is the most efficient.