RESUMO
1. The intracellular volume in granulation tissue was about 15% of the total urea space. 2. The experimental granuloma has a greater ability to retain amino acids during the proliferation phase than later during the synthesis of collagen. 3. The synthesis of collagen and other proteins by granulation tissue is related to the concentrations of proline and glutamic acid in the medium. 4. The rate of synthesis of proline from glutamic acid in granulation-tissue slices is greatest during collagen synthesis. It is enhanced by lactate. 5. Extracellular cations influence the synthesis of collagen and ouabain is inhibitory. Synthesis of other proteins is less sensitive in this respect. 6. It is suggested that the synthesis of collagen is related to the supply of certain amino acids, especially proline, and hence to the redox balance, and also to the function of the cell wall.
Assuntos
Aminoácidos/metabolismo , Granuloma/metabolismo , Aminoácidos/análise , Animais , Transporte Biológico , Isótopos de Carbono , Celulose , Cromatografia por Troca Iônica , Colágeno/biossíntese , Espaço Extracelular , Glutamatos/metabolismo , Granuloma/induzido quimicamente , Hidroxiprolina/metabolismo , Lactatos/farmacologia , Masculino , Monossacarídeos , Ouabaína/farmacologia , Potássio/farmacologia , Prolina/metabolismo , Biossíntese de Proteínas , Ratos , Sódio/farmacologia , Fatores de Tempo , Trítio , UreiaRESUMO
The survey of the fragments obtained from pepsin-digested, denatured rat tail tendon collagen is completed. Three additional fragments could be renatured and two of them (490 A and 670 A) were located in tropocollagen by electron microscopy. Data are given on the amino acid compositions of the various fractions. Certain fragments probably originated from the associated non-collagenous material belonging to the "acidic structural proteins".
Assuntos
Colágeno/ultraestrutura , Pepsina A , Aminoácidos/análise , Animais , Colágeno/química , Microscopia Eletrônica , Fragmentos de Peptídeos/química , Fragmentos de Peptídeos/ultraestrutura , Desnaturação Proteica , RatosRESUMO
1. The metabolism of incubated slices of sponge-induced granulation tissue, harvested 4-90 days after the implantation, was studied with special reference to the capacity of collagen synthesis and to the energy metabolism. Data are also given on the nucleic acid contents during the observation period. Three metabolic phases were evident. 2. The viability of the slices for the synthesis of collagen was studied in various conditions. Freezing and homogenization destroyed the capacity of the tissue to incorporate proline into collagen. 3. Consumption of oxygen reached the maximum at 30-40 days. There was evidence that the pentose phosphate cycle was important, especially during the phases of the proliferation and the involution. The formation of lactic acid was maximal at about 20 days. 4. The capacity to incorporate proline into collagen hydroxyproline in vitro was limited to a relatively short period at 10-30 days. 5. The synthesis of collagen was dependent on the supply of oxygen and glucose, which latter could be replaced in the incubation medium by other monosaccharides but not by the metabolites of glucose or tricarboxylic acid-cycle intermediates.
