[Correlation Between Index Components and Climatic Factors of Pseudostellariae Radix].

Objective: To analyze the correlation between index components and climatic factors of Pseudostellariae Radix. Methods: Correlation analysis ( CA), partial least square (PLS) and PCA ordination were applied to analyze the correlation of index components in Pseudostellariae Radix and climatic factors, and to explore the main climatic factors affecting the accumulation of index components in Pseudostellariae Radix. Results: The origin and harvest time had influence on index components. The monthly minimum temperature, monthly average humidity, monthly precipitation and the annual precipitation were the dominant factors influencing the content of index components. Conclusion: Through the positive and negative correlation between climatic factors and index components, it could explain the rationality of traditional harvest time and the reason of high content of heterophyllin B in Pseudostellariae Radix from Jiangsu Jurong.

[Simultaneous determination of thirteen components in Polygoni Multifori Caulis by UPLC-MS/MS].

A comprehensive analytical method based on UPLC-MS/MS was developed for the simultaneous determination of thirteen components including three stilbenes (stilbeneglucoside, polydatin, resveratrol), four anthraquinones (emodin, physcion, emodin-8-ß-D-glucopyranoside, aloe-emodin), five flavonoids (epicatechin, rutin, hyperoside, astragalin,quercetin) and one phenolic acid (gallic acid) in Polygoni Multifori Caulis.The separation was carried out on a Waters BEH C18 column（2.1 mm×100 mm,1.7 µm）with gradient elution of acetonitrile-water (0.1% acetic acid) at a flow rate of 0.25 mL•min⁻¹, and column temperature was 35 ℃. The target compounds were analyzed by multiple reaction monitoring (MRM) mode. TOPSIS analysis ware performed to evaluate the samples from different areas and commercial herbs according to the contents of thirteen components. The correlation coefficients of all the calibration curves were higher than 0.991 5. The average recoveries ranged from 95.24% to 102.3%, and the relative standard deviations were less than 5%. The result of TOPSIS analysis showed that the comprehensive quality of Polygoni Multifori Caulis sample from Guangzhou was better. The developed method with good repeatability and accuracy was suitable for the simultaneous determination of multiple functional substances, which provided a new basis for the comprehensive assessment and overall control of the quality of Polygoni Multifori Caulis.

Dynamic accumulation analysis on bioactive constituents of Polygonum multiflorum in different collection periods.

To study the dynamic change law of bioactive constituents from Polygonum multiflorum, and to explore the optimal harvest period of P. multiflorum. Determination of stilhene glucoside, anthraquinones and catechin from P. multiflorum in different harvest times by MEKC-DAD, and principal component analysis (PCA) was used to comprehensive evaluation for bioactive constituents. There are obvious differences among the contents of active ingredients in various collecting periods samples, the content of stilbene glucoside was the highest in November, the total content of combined anthraquinone was the highest in November and December, the content of catechin was the highest in September. The comprehensive evaluation index obtained with principal component analysis showed that the sample collected in November is significantly higher than those with other samples. The optimal harvest period of P. multiflorum is November.

[QTRAP LC-MS/MS Analytical Study on Nucleosides and Nucleobases of Pseudostellariae Radix Cultivated in Different Idioplasm Resources].

OBJECTIVE: To analyze nucleosides and nucleobases of Pseudostellariae Radix cultivated in different idibplasni resources and to compare the differences. METHODS: QTRAP LC-MS/MS method was applied for the analysis of 13 kinds of nucleosides and nucleobases in Pseudostellariae Radix and the data obtained was analyzed by SPSS 16. 0 software. RESULTS: There were some differences between Pseudostellariae Radix cultivated in different idioplasm resources. The highest amount of nucleosides and nucleobases was ZS2 which came from Zherong in Fujian Province. The total content of nucleosides and nucleobases in the sample from Shibing in Guizhou Province was the lowest. There was little difference between ZS1 (Zherong in Fujian Province) and XC(Xuancheng in Anhui Province). CONCLUSION: This study provides evidence for the influence of eco-environment on the metabolites of Pseudostellariae Radix.

[Dynamic Changes of Nucleosides and Nucleobases in Different Harvest Periods of Polygoni Multiflori Radix by UPLC-QTRAP-MS/MS].

OBJECTIVE: To analyze the dynamic changes of nucleosides and nucleobases in Polygoni Multiflori Radix harvested in different periods. METHODS: UPLC-QTRAP-MS/MS method was applied for the analysis of nine kinds of nucleosides and nucleobases in Polygoni Multiflori Radix. RESULTS: The content of uridine, adenine, guanosine and cytidine was higher in Polygoni Multiflori Radix harvested in different periods and assumed some difference. The trends of nucleosides and nucleobases from Polygoni Multiflori Radix according to the peak valley shape changed. The highest contents of them were in December. CONCLUSION: The accumulation of nucleosides and nucleobases in Polygoni Multiflori Radix is closely related to its growth cycle. It is found to be basically the same as that obtained when the herb is collected during the conventional collecting time.