MYH10 Combines with MYH9 to Recruit USP45 by Deubiquitinating Snail and Promotes Serous Ovarian Cancer Carcinogenesis, Progression, and Cisplatin Resistance.

The poor prognosis of serous ovarian cancer (SOC) is due to its high invasive capacity and cisplatin resistance of SOC cells, whereas the molecular mechanisms remain poorly understood. In the present study, the expression and function of non-muscle myosin heavy chain IIB (MYH10) in SOC are identified by immunohistochemistry, in vitro, and in vivo studies, respectively. The mechanism of MYH10 is demonstrated by co-immunoprecipitation, GST pull-down, confocal laser assays, and so on. The results show that the knockdown of MYH10 suppressed SOC cell proliferation, migration, invasion, metastasis, and cisplatin resistance both in vivo and in vitro. Further studies confirm that the MYH10 protein functional domain combines with non-muscle myosin heavy chain IIA (MYH9) to recruit the deubiquitinating enzyme Ubiquitin-specific proteases 45 and deubiquitinates snail to inhibit snail degradation, eventually promoting tumorigenesis, progression, and cisplatin resistance in SOC. In clinical samples, MYH10 expression is significantly elevated in SOC samples compared to the paratumor samples. And the expression of MYH10 is positively correlated with MYH9 expression. MYH10+/MYH9+ co-expression is an independent prognostic factor for predicting SOC patient survival. These findings uncover a key role of the MYH10-MYH9-snail axis in SOC carcinogenesis, progression, and cisplatin resistance, and provide potential novel therapeutic targets for SOC intervention.

FusionSegNet: Fusing global foot features and local wound features to diagnose diabetic foot.

The Diabetic Foot (DF) is threatening every diabetic patient's health. Every year, more than one million people suffer amputation in the world due to lack of timely diagnosis of DF. Diagnosing DF at early stage is very essential to improve the survival rate and quality of patients. However, it is easy for inexperienced doctors to confuse DFU wounds and other specific ulcer wounds when there is a lack of patients' health records in underdeveloped areas. It is of great value to distinguish diabetic foot ulcer from chronic wounds. And the characteristics of deep learning can be well applied in this field. In this paper, we propose the FusionSegNet fusing global foot features and local wound features to identify DF images from foot ulcer images. In particular, we apply a wound segmentation module to segment foot ulcer wounds, which guides the network to pay attention to wound area. T he FusionSegNet combines two kinds of features to make a final prediction. Our method is evaluated upon our dataset collected by Shanghai Municipal Eighth People's Hospital in clinical environment. In the training-validation stage, we collect 1211 images for a 5-fold cross-validation. Our method can classify DF images and non-DF images with the area under the receiver operating characteristic curve (AUC) value of 98.93%, accuracy of 95.78%, sensitivity of 94.27%, specificity of 96.88%, and F1-score of 94.91%. With the excellent performance, the proposed method can accurately extract wound features and greatly improve the classification performance. In general, the method proposed in this paper can help clinicians make more accurate judgments of diabetic foot and has great potential in clinical auxiliary diagnosis.

A Transvenous Endovascular Approach in Straight Sinus Has Minor Impacts on Chordae Willisii.

Cerebral dural sinuses contain different types of chordae willisii (CW). The transvenous endovascular approach, which has become an optimal method for the treatment of cerebrovascular diseases, such as malformation, fistula, and chronic intracranial hypertension, due to sinus thromboses, frequently uses retrograde navigation through dural sinuses. Whether or how much the endoscopic procedure damages the chordae willisii is often not well-assessed. In our study, an overall number of 38 cadaveric heads were analyzed for the distribution and features of the chordae willisii in the straight sinus. We used an endoscope on these samples mimicking a mechanical thrombectomy procedure performed in the straight sinus. Both endoscopic gross observation and light microscopic histological examination were used to assess the damages to the chordae willisii by the procedure. We found that the valve-like lamellae and longitudinal lamellae structures were mainly found in the posterior part of straight sinus whereas trabeculae were present in both anterior and posterior portions. We treated a group of samples with a stent and another with a balloon. The stent-treated group had a significantly higher rate of Grade 1 damage comparing with the balloon-treated group (p = 0.02). The incidence of damage to the surface of chordae willisii was also higher in the stent-treated group (p = 0.00). Neither the use of stent nor of balloon increased the rate of damage to chordae willisii during repeated experiments. These findings indicated that stent or balloon navigation through the straight sinus can cause minor damages to the chordae willisii and frequent uses of retrograde navigation through the straight sinus do not appear to increase the rates of damage to chordae willisii.