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1.
Clin Transl Oncol ; 18(4): 375-80, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26329292

RESUMO

AIM: To investigate the role of human epididymis protein 4 (HE4) in the diagnosis and prognosis of patients with locally advanced non-small cell lung cancer (LA-NSCLC) receiving concurrent chemoradiotherapy (CRT). METHODS: A total of 218 patients with LA-NSCLC were enrolled. All patients underwent CRT. The treatment response to CRT was evaluated. The prognosis analysis was performed using relapse-free survival (RFS) and overall survival [1]. RESULTS: Our data show that the serum HE4 can discriminate patients who respond well to CRT from those who respond poorly. Higher serum HE4 had dramatically increased risk of being non-responders to CRT. Serum HE4 level is also associated with prognosis of patients after CRT. Patients with high HE4 level had shorter RFS and OS compared to those with low HE4 level. CONCLUSION: Our data suggest that serum HE4 may be a useful prognostic biomarker for LA-NSCLC patients who underwent CRT.


Assuntos
Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Biomarcadores Tumorais/metabolismo , Carcinoma Pulmonar de Células não Pequenas/patologia , Quimiorradioterapia , Neoplasias Pulmonares/patologia , Recidiva Local de Neoplasia/patologia , Proteínas/metabolismo , Adenocarcinoma/metabolismo , Adenocarcinoma/patologia , Adenocarcinoma/terapia , Carboplatina/administração & dosagem , Carcinoma Pulmonar de Células não Pequenas/metabolismo , Carcinoma Pulmonar de Células não Pequenas/terapia , Carcinoma de Células Escamosas/metabolismo , Carcinoma de Células Escamosas/patologia , Carcinoma de Células Escamosas/terapia , Estudos de Casos e Controles , Cisplatino/administração & dosagem , Ensaio de Imunoadsorção Enzimática , Feminino , Seguimentos , Humanos , Neoplasias Pulmonares/metabolismo , Neoplasias Pulmonares/terapia , Masculino , Pessoa de Meia-Idade , Recidiva Local de Neoplasia/metabolismo , Recidiva Local de Neoplasia/terapia , Estadiamento de Neoplasias , Paclitaxel/administração & dosagem , Prognóstico , Curva ROC , Estudos Retrospectivos , Taxa de Sobrevida , Vimblastina/administração & dosagem , Vimblastina/análogos & derivados , Vinorelbina , Proteína 2 do Domínio Central WAP de Quatro Dissulfetos
2.
Analyst ; 120(4): 1115-24, 1995 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-7771675

RESUMO

The theory and methodology of a three-level orthogonal array design as a chemometric method for the optimization of analytical procedures were developed. In the theoretical section, firstly, the matrix of a three-level orthogonal array design is described and orthogonality is proved by a quadratic regression model. Next, the assignment of experiments in a three-level orthogonal array design and the use of the triangular table associated with the corresponding orthogonal array matrix are illustrated, followed by the data analysis strategy, in which significance of the different factor effects is quantitatively evaluated by the analysis of variance (ANOVA) technique and the percentage contribution method. Then, a quadratic regression equation representing the response surface is established to estimate each factor that has a significant influence. Finally, on the basis of the quadratic regression equation established, the derivative algorithm is used to find the optimum value for each variable considered. In the application section, microwave dissolution for the determination of selenium in biological samples by hydride generation atomic absorption spectrometry is employed, as a practical example, to demonstrate the application of the proposed three-level orthogonal array design in analytical chemistry.


Assuntos
Técnicas de Química Analítica/estatística & dados numéricos , Micro-Ondas , Selênio/análise , Solubilidade
3.
Talanta ; 41(11): 1917-27, 1994 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-18966150

RESUMO

It is the purpose of the present work to provide information on the four-level orthogonal array design and data analysis for the optimization of analytical procedures. In the theoretical part, the construction and characteristics of the OA(16)(4(5)) matrix is described in detail, followed by the data analysis strategy, in which the significance of the different factors is quantitatively evaluated by an analysis of variance (ANOVA) method including per cent contribution, and the difference among four levels for each factor is determined by Duncan's multiple F test. Furthermore, a third-order polynomial model representing response surface is established to estimate the effects for the factors with significant influences. In the application part, the proposed four-level orthogonal array design and data analysis method were applied to optimize polarographic reaction system for phosphorus determination. By conducting 16 preplanned experiments that span the maximum working range of the system, the best experimental conditions for achieving the largest response can be obtained. The expected value for each experimental trial calculated by the third-order regression equation established is in good agreement with the corresponding experimental value. To confirm the validity of the optimization procedure, additional experiments using the recommended conditions were performed. The results demonstrate that satisfactory results can be acquired. Therefore, the proposed four-level orthogonal array design as a chemometric approach to optimize the polarographic reaction system for phosphorus determination is rather efficient and effective.

4.
Talanta ; 41(2): 195-200, 1994 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-18965907

RESUMO

Four microwave digestion methods of fish tissue for selenium determination by hydride generation atomic absorption spectrometry were compared, in which potassium hexacyanoferrate(III) was chosen as a masking agent for eliminating matrix interferences. The results showed that the methods employing HNO(3)/H(2)O(2), HNO(3)/K(2)S(2)O(8)/H(2)O(2) and HNO(3)/H(3)PO(4)/H(2)O(2) digestion media were unreliable. However, the decomposition using the digestion media of HNO(3)/H(2)SO(4)/H(2)O(2) enabled adequate digestion of fish tissue and retention of selenium in a state amenable for determination. Therefore, the digestion procedures with HNO(3)/H(2)SO(4)/H(2)O(2) media are proposed for the determination of selenium in fish tissue by hydride generation atomic absorption spectrometry. The recoveries of the spiked samples investigated ranged from 90 to 102%. The result obtained from analyzing the NIES CRM No. 6 mussel was in good agreement with the reference value (reference value: 1.5 mug/g; found: 1.45 +/- 0.05 mug/g). The limit of detection for selenium was 0.03 mug/g dry mass for a 100 mg sample. The contents of selenium in local fish species investigated ranged from 0.49 to 2.90 mug/g, and the relative standard deviation for the determination of selenium was less than 8%.

5.
Talanta ; 41(1): 53-8, 1994 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-18965886

RESUMO

In KIO(3)NH(3)NH(4)Cl medium, the selenium complex Se(O)SO(2-)(3), resulted from the reaction of selenite and sulphite in acid solution, gave a catalytic wave, which was applied to the determination of selenium in fish by differential pulse polarography. The sample was decomposed using the HNO(3)/H(2)SO(4)/H(2)O(2) digestion mixture in a closed PTFE digestion vessel with microwave heating. The detection limit was 0.06 mug/dm(3). The calibration curve was linear up to 8 mug/dm(3). Selenate present was reduced with hot hydrochloric acid to selenite. The recoveries of the selenite and selenate in two spiked samples investigated ranged from 91 to 104%. The NIES CRM No. 6 mussel was analyzed and the results obtained agreed well with the reference value (reference value: 1.5 mug/g; found: 1.43 +/- 0.05 mug/g). The results obtained by differential pulse polarography were in good agreement with those found by hydride generation atomic absorption spectrometry.

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