RESUMO
The molecular mechanisms of rise, development, and correction of some uninfectious illness of ungenetical nature and conception of ligand pathology are analyzed. The influence of molecular geometry change upon the pathology development and biophysical aspects of drugs searching are considered.
Assuntos
Doença/etiologia , Desenho de Fármacos , Fenômenos Biofísicos , Biofísica , Humanos , LigantesRESUMO
We calculated 219 topological, electronic, and steric indices for each of 59 studied embryotoxic benzylalkylamines and indolamines. Statistically significant equations correlating embryotoxicity and five calculated parameters were obtained using the method of step multiple regression. The prognostic power of the equation was 88-90%. Discriminant functions obtained by step discriminant analysis allowed prediction of the superactivity of benzylalkylamines and indolamines with 83-87% probability.
Assuntos
Aminas Biogênicas/toxicidade , Embrião não Mamífero/efeitos dos fármacos , Animais , Análise Discriminante , Prognóstico , Análise de Regressão , Ouriços-do-Mar/efeitos dos fármacos , Ouriços-do-Mar/embriologia , Software , Relação Estrutura-AtividadeRESUMO
Discriminant functions were obtained on the basis of experimental data on the superactivity of synthetic analogues of the natural biogenic amines (26 active and 33 superactive derivatives of benzene and indole), which allow prognostication of the superactivity of embryotoxic compounds with respect to sea urchin embryos on the basis of calculated parameters of the molecules. During reclassification of molecules of the learning sample, the percentage of correct recognition is 73.1 for active, 87.9 for superactive, and 81.4% for the entire data bank. The prognostication capacity of the obtained functions is 73-80%.
Assuntos
Aminas Biogênicas/toxicidade , Simulação por Computador , Embrião não Mamífero/efeitos dos fármacos , Modelos Biológicos , Animais , Análise Discriminante , Prognóstico , Ouriços-do-Mar , Relação Estrutura-AtividadeRESUMO
The discriminant functions allowing to predict the phenomenon of superactivity for some embryotoxic neurochemicals by means of tabular electronic and lipophilic parameters are received. The prognosis possibility of the received functions for benzene and indole derivatives on the sea urchin embryos is about 93%.
Assuntos
Derivados de Benzeno/toxicidade , Embrião não Mamífero/efeitos dos fármacos , Indóis/toxicidade , Animais , Derivados de Benzeno/química , Análise Discriminante , Indóis/química , Prognóstico , Ouriços-do-Mar , Relação Estrutura-AtividadeRESUMO
A hypothesis is suggested on the positive effect of light radiation on the formation of protective immune mechanism related to the photophysiological destruction of porphyrins. References to experimental data are given which illustrate the possibility for existence of such a mechanism.
Assuntos
Imunidade Inata/efeitos da radiação , Imunização , Luz Solar , Bilirrubina/efeitos da radiação , Humanos , Imunidade Inata/fisiologia , Oxigênio/efeitos da radiação , Fotoquímica , Porfirinas/imunologia , Porfirinas/efeitos da radiação , Oxigênio SingleteRESUMO
Phototoxic side effects of a number of different drugs and natural photosensitizers [correction of photosensors] (psoralenes, psychotropic phenotiazines, anti-malaria drugs, porphirines, adriamycine, etc.) have been analyzed. The ways of study of sensory harmlessness of drugs have been discussed.
Assuntos
Olho/efeitos dos fármacos , Luz/efeitos adversos , Radiossensibilizantes/efeitos adversos , Olho/efeitos da radiação , Humanos , Fotoquímica , Relação Estrutura-AtividadeRESUMO
The shortcomings of available approaches to determination of the character of chemical compounds biological activity according to calculated, tabular and experimental parameters have been analyzed using statistic methods. Some ways of available algorithms improving are given.
Assuntos
Psicotrópicos/farmacologia , Algoritmos , Animais , Análise Discriminante , Métodos , PrognósticoRESUMO
Geometric flexibility has been analyzed in nucleotides with 46 (deoxyribofuranose) and 74 (ribofuranose) conformers and tentatively (within most stable conformation of five-element ring in nucleic acids) 4 and 8 geometric isomers, respectively. Possible biological role of geometric isomers of deoxyribofuranose during DNA functioning has been discussed.
Assuntos
DNA Viral/química , DNA/química , Desoxirribose/química , Ribose/química , Animais , Configuração de Carboidratos , Isomerismo , Conformação de Ácido NucleicoRESUMO
The present study of 2,387 recordings of 12 anterior teeth in 53 patients indicates that the incisal edge is the optimal placement-site for the electric pulp tester to determine the lowest response threshold. The results show significant individual variations in the lowest threshold responses of the cervical-third, middle-third, incisal one-third, and incisal edge sites on a tooth, with a confidence level of 99%, according to the analysis of variance. The maxillary teeth gave a higher response threshold than the mandibular teeth and different types of teeth (canines and incisors) had statistically significant different response thresholds. The application of the electric pulp tester to the incisal-edge region with exposed dentin produced the most significant decrease in the threshold response.
Assuntos
Dente Canino , Teste da Polpa Dentária/instrumentação , Eletrodos , Incisivo , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Dente Canino/anatomia & histologia , Teste da Polpa Dentária/métodos , Limiar Diferencial , Estimulação Elétrica , Humanos , Incisivo/anatomia & histologia , Pessoa de Meia-Idade , Abrasão Dentária/fisiopatologiaRESUMO
Some aspects of the strategy of initial search of biologically active compounds at mass tests of chemical compounds have been discussed. Different approaches to pre-experimental estimation of biological activity using mathematical statistics and computer analysis have been analysed.
Assuntos
Avaliação Pré-Clínica de Medicamentos/métodos , Animais , Simulação por Computador , Desenho de Fármacos , Avaliação Pré-Clínica de Medicamentos/estatística & dados numéricos , Processamento Eletrônico de Dados , Modelos Biológicos , Modelos Químicos , Projetos de Pesquisa , Relação Estrutura-AtividadeAssuntos
Biologia Molecular , Penicilinas/farmacologia , Albumina Sérica/farmacologia , Sítios de Ligação/efeitos dos fármacos , Interações Medicamentosas , Espectroscopia de Ressonância de Spin Eletrônica , Humanos , Ligantes , Matemática , Conformação Molecular , Ligação Proteica/efeitos dos fármacos , TermodinâmicaRESUMO
The possibility of a calorimetric determination of the number of homogeneous independent binding sites on the protein molecule surface is presented, together with the possibility of the determination of interaction heat and association constants for the binding of small molecules to one such site. Thermodynamic characteristics of interaction of 10 penicillins and methyl orange with primary and secondary binding sites of bovine serum albumin have been obtained using this technique. The data show that hydrogen bonds between the component parts of the complexes studied are absent. The main contribution to free energy change at forming these complexes is made by hydrophobic interactions. Electrostatic forces are also of some importance, their contribution into the complex formation is more significant in the case of quinacillin and carbenicillin which have an additional charged carboxyl group.
Assuntos
Penicilinas , Soroalbumina Bovina , Sítios de Ligação , Calorimetria , Cinética , Matemática , Ligação Proteica , Relação Estrutura-Atividade , TermodinâmicaRESUMO
Antibacterial activity of 2 natural and 12 semisynthetic penicillins against 5 strains of grampositive bacteria was determined and quantum chemical estimation of their molecules was performed with the Hukkel method. The data were indicative of the fact that antibacterial activity of the penicillins was connected with the acceptor properties of their molecules.