[FEATURES OF THE IMMUNE GENETIC PARAMETERS IN WORKERS IN NON-FERROUS METAL INDUSTRY].

There was performed a comparative analysis of immunogenetic indices in non-ferrous metallurgy employees under the exposure to different combinations of harmful occupational factors. The combined effect of chlorine and vanadium fumes, noise, overall vibration appeared to be associated with the gene polymorphism of cytokine regulation--VEGF and TNF (p < 0.05). In workers the combination offactors such as dust containing silicon dioxide, noise, elevated environmental thermal load was associated with cytochrome p450 gene polymorphism, allele variation ofwhich is formed owing to the homozygous genotype. At the same time there was observed an excess production of specific antibodies to vanadium and silicon, significantly differed from that of the indices in the reference group. There are proposed genetic (CYP1A1, VEGF TNFalfa) and immunological (IgG to vanadium and silicon) indices as markers of susceptibility and effect in health risk assessment of different combinations of harmful occupational factors, which will allow to increase the availability of laboratory control during surveillance activities at the objects.

[Characteristics of regulatory system in children exposed to the environmental chemical factors].

In children residing in areas with a high content of a number of aromatic hydrocarbons in ambient air and organochlorine compounds in drinking water there were studied the blood levels of these compounds, as well as the assessment of the indices of the immune and neuroendocrine systems was performed. The higher blood content of phenol and formaldehyde has been established and there was identified an array of organochlorine and aromatic compounds not detected in the control group children. In the blood of the children of a study group there was found an imbalance of indices of cellular components of innate and adaptive immunity, pro- and anti-inflammatory cytokines, as well as increased concentrations of free thyroxine and serotonin in the blood serum, which indicates to a change in the functions of regulatory systems in children exposed to organochlorine and aromatic compounds.

A mathematical model of the immune and neuroendocrine systems mutual regulation under the technogenic chemical factors impact.

The concept of the triad regulatory metasystem, which includes the neuroendocrine and immune regulation systems, is currently generally accepted. Changes occurring in each of the regulatory systems in response to the impact of technogenic chemical factors are also well known. This paper presents mathematical models of the immune and neuroendocrine system functioning, using the interaction between these systems in response to bacterial invasion as an example, and changes in their performance under exposure to chemical factors, taking into account the stage of functional disorders in a producing organ, using the performance of the bone marrow as an example.

[Justifying genetic and immune markers of efficiency and sensitivity under combined exposure to risk factors in mining industry workers].

The authors evaluated and justified immunologic and genetic markers under combined exposure to risk factors in mining industry workers. Analysis covered polymorphism features of 29 genes with variant alleles possibly participating in occupationally conditioned diseases formation and serving as sensitivity markers of these diseases risk. The genes association selected demonstrates reliably changed polymorphism vs. the reference group (SOD2 superoxidedismutase gene, ANKK1 dophamine receptor gene, SULT1A1 sulphtransaminase gene, MTHFR methylene tetrahydrofolate reductase gene, VEGF endothelial growth factor gene, TNF-alpha tumor necrosis factor gene). Under combined exposure to occupational hazards (sylvinite dust, noise) in mining industry, this association can serve as adequate marking complex of sensitivity to development of occupationally conditioned diseases. Increased-production of immune cytokine regulation markers: tumor necrosis factor and vascular endothelial growth factor. Genes SOD2, ANKK1, SULT1A1, VEGF, TNFalpha are recommended as sensitivity markers, and the coded cytokines (tumor necrosis factor and endothelial growth factor) are proposed as effect markers in evaluation of health risk for workers in mining industry.

[Hygienic evaluation of immune and endocrine systems and modifications of their relationship in reproductive-age women working under exposure to chemical factors in activated carbon emissions].

The article presents results of the evaluation the changes in the relationships between immune and endocrine systems in reproductive-age women, working under exposure to chemical factors from activated carbon production. A significant increase of some chemical elements and compounds was found in blood that was associated with changes in the endocrine and immune status, as well as the presence of features in correlation parameters of these systems in reproductive-age women, working under exposure to chemical factors.

[Immunologic and genetic markers of exposure to aromatic hydrocarbons in workers].

Workers exposed to aromatic hydrocarbons appeared to have prevalence of heterozygous variants of CYP1A1 gene (9893 A/G) and tumor necrosis factor gene reliably higher vs. the reference group 2.5 and 3.3 times respectively, and level of anti-benzene antibodies (IgG) increased vs. the reference group. The data presented demonstrate negative immunogenetic associations of aromatic hydrocarbons influence on oil extraction operators.

[Effect of hydrocortisone on peripheral blood phagocytic cells under beta-adrenoreceptors blockade]. / Vliianie gidrokortizona na funktsii fagotsiruiushchikh kletok perifericheskoi krovi v usloviiakh blokady beta-adrenoretseptorov.

The effect of hydrocortisone (50 mg/kg body wt i.p.) under beta-adrenergic receptors blockade (four subcutaneous injections of propranolol in single dose of 5 mg/kg body wt with 3 h interval) on phagocytic activity and oxygen dependent microbicidal activity in NBT-test of peripheral blood phagocytic cells in male Wistar rats was investigated. It was established that hydrocortisone stimulated neutrophil phagocytic activity through 6, 24 and 48 h after hormone injection and decreased oxygen-dependent microbicidal activity of phagocytic cells in NBT-test. Hydrocortisone in vitro (500 ng/ml) decreased neutrophil phagocytic activity that indicated on realization of stimulating effect of hydrocortisone in vivo through complex of other indirect mechanisms. Administration of hydrocortisone led to depression of eosinophil phagocytosis and lesser decrease in monocyte phagocytic activity. Hydrocortisone effects were significantly modified under blockade of beta-adrenoceptors that indicated on its mediation by endogenous catecholamines through modulation of beta-adrenoceptor expression.

Effects of hydrocortisone on peritoneal phagocytizing cells in rats under conditions of beta-adrenoceptors blockade.

Hydrocortisone decreased the count of peritoneal mononuclear phagocytes and mast cells and total phagocytic activity of peritoneal phagocytes in rats, but had no effect on the nitroblue tetrazolium test. beta-Adrenoceptor blockade abolished the suppressive effect of hydrocortisone on phagocytosis and prevented the decrease in the count of mast cells, but markedly reduced the number of neutrophils and parameters of stimulated nitroblue tetrazolium test.