Pollination mechanism in Serapias with no pollinaria reconfiguration.

Orchidaceae, one of the most numerous families in the world's flora, have evolved various pollination strategies to favour cross-pollination, such as deceptive pollination and pollinarium reconfiguration. Among the terrestrial orchids of the Mediterranean, only species belonging to the genus Serapias show a strategy defined as shelter imitation. The floral elements form a tubular structure that insects use during their resting phases. The purpose of this article was to clarify the mechanisms that guarantee pollination with particular attention to the morphological interactions between orchids and pollinators and whether pollinaria reconfiguration is necessary in the promotion of cross-pollination in Serapias. Breeding system experiments and hand-pollination treatments indicated that Serapias was highly self-compatible, shows low value of natural fruit set and is pollinator limited. Time-lapse photos showed that the pollinarium had no refolding of the stipe or caudicle after its removal from the flower. The morphology of the flower determined the attack of the pollinarium on the occiput/vertex of insect. When the insect left the flower, the pollinarium was unable to encounter the stigma. When the insect made a second visit to another flower, the pollen masses of the first pollinarium ended up on the stigma and at the same time, the insect picked up a second pollinarium. Our observations and analyses suggested that morphological interactions between flower and pollinator are crucial to the success of pollination and to prevent self-pollination and thus that pollinarium reconfiguration is unnecessary in shelter deceptive orchids, such as Serapias species, for the promotion of cross-pollination. Serapias represent a case of interactions between plant and pollinator; the formation of the tubular shape of the flower is an essential preadaptation for the development of resting site mimicry originating exclusively in Serapias among Mediterranean orchids.

Pollinaria Reconfiguration Mechanism of Widespread Euro-Mediterranean Orchids: The Effects of Increasing Air Temperature.

Orchids are fascinating for many reasons: their reproductive strategies, their pollination systems and the various morphological adaptations they have evolved, including the presence of pollen grains agglomerated into two masses, called pollinia, which form a structure known as a pollinarium. After withdrawal from a flower, the pollinarium undergoes a bending movement such that the pollen masses become correctly orientated to strike the stigma. We evaluated the duration of pollinator visits to inflorescences and the effects of temperature on pollinaria reconfiguration in eight orchid species in order to analyze the effects of increasing air temperature on the changes in bending time, and thus on geitonogamy levels. The impact of temperature on insect behavior was not assessed because our priority was to understand the effects of temperature on the process of pollinaria reconfiguration. All the examined species showed natural reconfiguration times that were 1.7-3.0 times longer than the pollinator residency times. A higher temperature showed a reduction in bending time regardless of the species tested. However, the bending time was never shorter than the residence time of the insects on the flower. Our data showed that high temperatures had a limited effect on the pollinarium reconfiguration time, thus indicating that high temperatures had a limited effect on folding compared to the effect that it had on the viability of the pollen.