Drug repositioning: diacerein as a new therapeutic approach in a mice model of sciatic nerve injury.

BACKGROUND: Peripheral nerve injuries negatively impact the quality of life of patients, with no effective treatment available that accelerates sensorimotor recovery and promotes functional improvement and pain relief. The aim of this study was to evaluate the effects of diacerein (DIA) in an experimental mice model of sciatic nerve crush. METHOD: In this study, male Swiss mice were used, randomly separated into six groups as follows: FO (false-operated + vehicle); FO + DIA (false-operated + diacerein 30 mg/kg); SNI (sciatic nerve injury + vehicle); SNI + DIA in doses of 3, 10 and 30 mg/kg (sciatic nerve injury + treatment with diacerein in doses of 3-30 mg/kg). DIA or vehicle was administered 24 h after the surgical procedure, intragastrically, twice a day. The lesion of the right sciatic nerve was generated by crush. RESULTS: We found that the treatment of animals with DIA accelerated sensorimotor recovery of the animal. In addition, animals in the sciatic nerve injury + vehicle (SNI) group showed hopelessness, anhedonia, and lack of well-being, which were significantly inhibited by DIA treatment. The SNI group showed a reduction in the diameters of nerve fibers, axons, and myelin sheaths, while DIA treatment recovered all these parameters. In addition, the treatment of animals with DIA prevented an increase the levels of interleukin (IL)-1ß and a reduction in the levels of the brain-derived growth factor (BDNF). CONCLUSIONS: Treatment with DIA reduces hypersensitivity and depression like behaviors in animals. Furthermore, DIA promotes functional recovery and regulates IL-1ß and BDNF concentrations.

A study to assess the impact of cobas Liat point-of-care PCR assays (SARS-CoV-2 and Influenza A/B) on patient clinical management in the emergency department of the University of California at Davis Medical Center

BackgroundRapid detection of SARS-CoV-2 is crucial for reduction of transmission and clinical decision-making. The cobas(R) SARS-CoV-2 & Influenza A/B nucleic acid test for use on the cobas Liat(R) System is a rapid (20 minutes) point-of-care (POC) polymerase chain reaction (PCR) method. MethodsThis unblinded, pre-post study enrolled consecutive patients with symptoms/signs consistent with SARS-CoV-2 infection presenting to the University of California, Davis emergency department (ED). Outcomes following implementation of the cobas Liat SARS-CoV-2 & Influenza A/B test (intervention period: December 2020-May 2021) were compared with previous standard-of-care using centralized laboratory PCR methods (control period: April 2020-October 2020). ResultsElectronic health records of 8879 symptomatic patients were analyzed, comprising 4339 and 4540 patient visits and 538 and 638 positive SARS-CoV-2 PCR test results in the control and intervention periods, respectively. Compared with the control period, turnaround time (TAT) was shorter in the intervention period (median 0.98 vs 12.3 hours; p<0.0001). ED length of stay (LOS) was generally longer in the intervention period compared with the control period, but for those SARS-CoV-2-negative who were admitted, ED LOS was shorter (median 12.53 vs 17.93 hours; p<0.0001). Overall, the rate of anti-infective prescribing was also lower in the intervention period than in the control period (antibiotics only: 38.11% vs 44.55%; p<0.0001 and antivirals only: 3.13% vs 0.94%; p<0.0001). ConclusionThis real-world study confirms faster TAT with a POC PCR method in an emergency care setting and highlights the importance of rapid SARS-CoV-2 detection to aid patient management and inform treatment decisions. Clinical RelevanceThis study reports data collected from a quasi-experimental pre-post study using the electronic health records of patients presenting to the emergency department (ED) of the University of California at Davis Medical Center with symptoms or signs consistent with SARS-CoV-2 infection during their ED visit. The primary objective of this study was to determine if implementation of the point-of-care (POC) cobas(R) Liat(R) SARS-CoV-2 & Influenza A/B test for use on the cobas Liat System reduced the diagnostic turnaround time and/or length of stay for ED patients with suspected SARS-CoV-2 infection compared with the previous standards of care (batch-wise diagnostic testing using the cobas 6800 System and on-demand urgent testing on the GenMark Dx(R) ePlex(R) system in a centralized clinical laboratory). Ultimately, these data help to inform how implementation of POC molecular testing methods impact patient management.

COMPARATIVE PERFORMANCE OF TWO AUTOMATED MACHINE LEARNING PLATFORMS FOR COVID-19 DETECTION BY MALDI-TOF-MS

The 2019 novel coronavirus infectious disease (COVID-19) pandemic has resulted in an unsustainable need for diagnostic tests. Currently, molecular tests are the accepted standard for the detection of SARS-CoV-2. Mass spectrometry (MS) enhanced by machine learning (ML) has recently been postulated to serve as a rapid, high-throughput, and low-cost alternative to molecular methods. Automated ML is a novel approach that could move mass spectrometry techniques beyond the confines of traditional laboratory settings. However, it remains unknown how different automated ML platforms perform for COVID-19 MS analysis. To this end, the goal of our study is to compare algorithms produced by two commercial automated ML platforms (Platforms A and B). Our study consisted of MS data derived from 361 subjects with molecular confirmation of COVID-19 status including SARS-CoV-2 variants. The top optimized ML model with respect to positive percent agreement (PPA) within Platforms A and B exhibited an accuracy of 94.9%, PPA of 100%, negative percent agreement (NPA) of 93%, and an accuracy of 91.8%, PPA of 100%, and NPA of 89%, respectively. These results illustrate the MS methods robustness against SARS-CoV-2 variants and highlight similarities and differences in automated ML platforms in producing optimal predictive algorithms for a given dataset.

Persimmon anthracnose: a comparative study of aggressiveness on shoot and fruit among Colletotrichum horii isolates in southern Brazil / Antracnose do caquizeiro: um estudo comparativo da agressividade em ramos jovens e frutos entre isolados de Colletotrichum horii no sul do Brasil

ABSTRACT: The persimmon tree is known for its rusticity and productivity and was first introduced to Brazil in the late 19th century. However, anthracnose disease is causing immature fruit drop and severe disease symptoms in persimmon fruit, shoots, flowers, and twigs. The causal agent was first described as the fungal species, Colletotrichum horii, which was first confirmed using only the ITS region. In this study, we compared the aggressiveness of 13 isolates of Colletotrichum spp. obtained from fruit and shoots of persimmon grown in the Metropolitan Region of Curitiba, Paraná State, Brazil. A multilocus molecular analysis was carried out based on ITS, GPDH, and EF genes, and we confirmed that the isolates were confirmed as C. horii. All isolates were pathogenic for unwounded and wounded persimmon fruit but differed in aggressiveness. Only one isolate was non-pathogenic when inoculated into unwounded persimmon shoots. Most isolates caused cankers and shoot death whether shoots were wounded or unwounded. In this study, we emphasized the importance of shoots as a source of primary inoculum. In future studies, it will be critical to further elucidate the epidemiological basis of anthracnose disease by conducting field studies to establish a more effective strategy for disease control.

RESUMO: O caquizeiro foi introduzido no Brasil no final do século XIX. Esta planta é conhecida por sua rusticidade e produtividade. No entanto, a doença antracnose está causando queda e sintomas em frutos imaturos, ramos, flores e galhos no campo. O agente causal foi descrito inicialmente como Colletotrichum hori (confirmado usando apenas a região ITS) causando cancro de galho e sintomas de folhas. Neste estudo, comparamos a agressividade em caqui utilizando uma coleção de 13 isolados de Colletotrichum spp. obtidos de frutos e ramos de caqui da região metropolitana de Curitiba, Paraná, Brasil. Uma análise molecular multilocus foi realizada com base nos genes ITS, GPDH e EF, e os isolados foram confirmados como pertencentes a C. horii. Todos os isolados foram patogênicos em frutos não feridos e feridos, mas diferiram na agressividade. Apenas um isolado não foi patogênico quando inoculado sem ferimento em ramos. A maioria dos isolados foi capaz de causar cancro e morte de ramo, independentemente do ferimento. Neste estudo, enfatizamos a importância das novas brotações como fonte de inóculo primário. Para um próximo estudo, ainda é necessário ampliar as bases epidemiológicas com estudos em campo para estabelecer uma correta estratégia de controle desta doença.

Comparative analysis of Monilinia fructicola and M. laxa isolates from Brazil: monocyclic components of peach brown rot / Análise comparativa de isolados de Monilinia fructicola e M. laxa do Brasil: componentes monocíclicos da podridão parda

ABSTRACT: Brown rot is the most important disease of peaches in Brazil. The objective of this study was to compare the brown rot monocyclic components from Monilinia fructicola and M. laxa isolates from Brazil on peaches, due to the detection of M. laxa in the São Paulo production area. Conidia germination and pathogen sporulation were assessed in vitro under a temperature range of 5-35oC and wetness duration of 6-48h. Incubation and latent periods, disease incidence, disease severity and pathogen reproduction on peach fruit were evaluated under 10, 15, 20, 25 and 30oC and wetness duration of 6, 12 and 24h. Six of seven parameters of a generalised beta function fitted to conidia germination of M. fructicola and M. laxa were similar. Only the shape parameter was higher for M. fructicola indicating that the range of temperatures and wetness periods favourable for germination is wider for M. laxa than for M. fructicola. The optimum temperature for brown rot development caused by M. fructicola was 24.5oC and for. Monilinia laxa was 19.8oC. At 10oC M. laxa lesions produced more conidia than M. fructicola, and the opposite occurred at 30oC. The estimated maximum temperature for lesion development was also higher for M. fructicola than for M. laxa. M. fructicola is favored by warmer weather than M. laxa and the presence and impact of this specie in Brazil must be investigated especially in the South states.

RESUMO: Podridão parda é a doença mais importante de pêssegos no Brasil. O objetivo deste estudo foi comparar os componentes monocíclicos da podridão parda de isolados brasileiros de M. fructicola e M. laxa, devido à detecção de M. laxa em uma área de produção de São Paulo. A germinação de conídios e esporulação do patógeno foram avaliadas in vitro sob uma faixa de temperatura de 5-35oC e duração do molhamento de 6-48h. Os períodos de incubação e de latência, a incidência da doença, a severidade da doença e a reprodução do patógeno em frutos de pêssego foram avaliados em 10, 15, 20, 25 e 30ºC e duração de molhamento de 6, 12 e 24h. Seis dos sete parâmetros de uma função beta generalizada para germinação de conídios de M. fructicola e M. laxa foram semelhantes. Apenas o parâmetro de forma foi mais alto para M. fructicola indicando que a gama de temperaturas e períodos de molhamento favoráveis para germinação é maior para M. laxa do que para M. fructicola. A temperatura ideal para o desenvolvimento de podridão parda causada por M. fructicola foi 24,5oC e para Monilinia laxa foi 19,8oC. A 10oC lesões de M. laxa produziram mais conídios que as de M. fructicola, e o inverso ocorreu a 30oC. A temperatura máxima estimada para o desenvolvimento de lesões também foi maior para M. fructicola do que para M. laxa. M. fructicola é favorecido por um clima mais quente do que M. laxa e a presença e impacto deste patógeno no Brazil deve ser acompanhado em especial no estados do sul do país.

Gray mold in immature fig fruit: pathogenicity and growth temperature / Mofo cinzento em frutos imaturos de figo: patogenicidade e temperatura para crescimento

ABSTRACT: Several diseases can be associated with figs but recently a fruit rot was observed in green fruit. The purpose of this study was to determine the pathogenicity of Botrytis sp., to quantify incubation period (IP) and latent period (LP), to verify the optimum temperature for mycelial growth, and to identify the different species of Botrytis sp. isolated from immature figs. Botrytis sp. isolated from figs proved to be pathogenic to immature fruit with and without wounding the fruit surface and ostiole. The IP period was 3 days on fruit with wounds and 5 days on fruit inoculated within the ostiole (without wound). The LP was 6 days in all treatments. The optimum temperature for mycelial growth was 18°C. Inferred from sequences of a segment comprising the ITS region of ribosomal DNA concluded that the isolates are Botrytis cinerea.

RESUMO: Várias doenças podem estar associadas com figos, mas recentemente uma podridão dos frutos foi observada em frutos verdes. O objetivo deste estudo foi determinar a patogenicidade de Botrytis sp., quantificar o período de incubação (PI) e o período de latência (PL), verificar a temperatura ótima para o crescimento micelial e identificar as diferentes espécies de Botrytis sp. isoladas a partir de frutos imaturos de figo. Botrytis sp. isolado a partir de figos provou ser patogênico em frutos imaturos com e sem ferimento na superfície dos frutos e no ostíolo. O PI foi de 3 dias em frutos com ferimento e 5 dias em frutos inoculados no ostíolo e sem ferimento. O PL foi de 6 dias em todos os tratamentos. A temperatura ótima de crescimento micelial foi18°C. A partir de sequências de um segmento que compreende a região de ITS do DNA ribossomal, concluiu-se que os isolados são Botrytis cinerea.

Danos na soja causada por míldio / Damage to soybean caused by downy mildew

RESUMO: O míldio da soja é disseminado em todo mundo. Entretanto, o dano que ele causa não tem sido estudado no Brasil. O objetivo deste trabalho foi avaliar quais componentes de rendimento da soja são afetados por essa doença e determinar o coeficiente de dano. Dois experimentos foram conduzidos, um em Castro (2006/07) e outro em Ponta Grossa (2007/08), no Estado do Paraná, Brasil. O delineamento experimental foi de blocos ao acaso com (i) seis tratamentos e cinco repetições para os experimentos de campo, safras 2006/07, e (ii) oito tratamentos e quatro repetições para 2007/08. Foram aplicados fosfito de potássio (750g ia ha-1), propamocarb + fenamidona (900g ia ha-1) e mancozeb (2,400g ia ha-1). Em todos os tratamentos, a severidade máxima da infecção pelo míldio ocorreu no estádio R5.3 e a porcentagem de severidade variou entre 0 e 43%. O gradiente da doença foi obtido apenas na segunda safra. Equações de danos foram geradas para o peso de grãos e produtividade. O número de grãos por vagem e o número de vagens por planta não foram afetados pelo míldio mas o peso de 1000 sementes por planta foi reduzido linearmente com o aumento da severidade do míldio.

ABSTRACT: Downy mildew is widespread throughout the world. However, the damage that it causes has not been studied in Brazil yet. The objective of this work was to evaluate which components of soybean yield are affected by downy mildew and to determine the coefficient of damage. Two field experiments were conducted in Castro (2006/07) and Ponta Grossa (2007/08) in the state of Parana, Brazil. The experimental design consisted of completely randomized blocks of (i) six treatments and five replications for the 2006/07 and (ii) eight treatments and four replications for the 2007/08 field experiments. Potassium phosphite (750g a.i. ha-1), propamocarb + fenamidone (900g a.i. ha-1) and mancozeb (2400g a.i. ha-1) were applied. In all of the treatments, the maximum severity of downy mildew infection occurred at growth stage R5.3, and the percentage of severity ranged between 0 and 43%. The disease gradient was obtained only on the second season. Equations of damage were generated for the yield and grain weight. The number of grains per pod and the number of pods per plant were not affected by downy mildew but, the weight of 1000 seeds per plant was reduced linearly with increasing of mildew severity.

Reação de cultivares e época de avaliação da ferrugem alaranjada da cana-de-açúcar / Reaction of commercials cultivars and assessment date of orange rust of sugarcane

Os objetivos deste estudo foram avaliar a reação de 28 cultivares de cana-de-açúcar à ferrugem alaranjada, em campo, estabelecendo uma época de avaliação adequada para os programas de melhoramento, e determinar os períodos de incubação e latência, incidência e severidade na cultivar padrão suscetível, em casa-de-vegetação. No campo, a severidade foi avaliada em cana-planta (dez meses) e em cana-soca (três, cinco, sete e nove meses). A partir destes dados, calculou-se a área abaixo da curva de progresso da doença (AACPD). Para estabelecer a época de avaliação adequada, calculou-se a correlação de Pearson entre os resíduos das datas com os da AACPD. Em casa-de-vegetação, inoculou-se a cultivar RB72454 e avaliou-se incidência e severidade. Das cultivares avaliadas, 14 foram suscetíveis à ferrugem alaranjada, sendo discriminadas em quatro grupos de suscetibilidade. A avaliação em maio (cana-soca, aos nove meses), foi a que mais se correlacionou com a AACPD, sendo a época adequada para avaliar a reação de cultivares à doença, no norte do Estado do Paraná. O período de incubação e latência na cultivar padrão suscetível foi de sete e dez dias, respectivamente. A cultivar RB72454 é a única altamente suscetível à ferrugem alaranjada e as demais avaliadas apresentam menores graus de suscetibilidade.

The aims of this study were to assess the reaction of 28 sugarcane cultivars to orange rust, establishing the most appropriate date for the breeding programs assess the reaction of cultivars, and, in greenhouse, determine the incubation and latency periods, the incidence and the severity in the susceptible standard cultivar. In the field, the severity was assessed in plant cane (ten months) and ratoon crops (three, five, seven and nine months). The area under the disease progress curve (AUDPC) was calculated from these data. To establish the appropriate date of assessment, the Pearson correlation was calculated between the residues of assessment dates and the AUDPC. In greenhouse, the cultivar RB72454 was inoculated and the incidence and severity were assessed. Among 28 cultivars, 14 showed some degree of susceptibility of orange rust and they may be discriminated into four groups of susceptibility. The assessment in May was the most correlated with AUDPC, being the appropriate date for assessing susceptibility of cultivars to orange rust, in the northern region of Paraná State. The incubation and latency periods in the susceptible standard cultivar were seven and ten days, respectively. The cultivar RB72454 is the only highly susceptible to orange rust and other cultivars assessed have lower degrees of susceptibility.

Extratos, decoctos e óleos essenciais de plantas medicinais e aromáticas na inibição de Glomerella cingulata e Colletotrichum gloeosporioides de frutos de goiaba / Extracts, decoctions and essential oils of medicinal and aromatic plants in the inhibition of Colletotrichum gloeosporioides and Glomerella cingulata isolates from guava fruits

A principal doença da goiaba (Psidium guajava L.), após a colheita, é a antracnose, causada por Glomerella cingulata e Colletotrichum gloeosporioides. Estes patógenos e o resíduo de fungicidas em frutos são considerados os principais problemas para a exportaçãodesta fruta. Neste trabalho, foi avaliado o efeito fungitóxico de extratos, decoctos e óleos essenciais de plantas medicinais e aromáticas, no crescimento micelial dos patógenos, in vitro, recomendados como alternativa para o controle químico em pós-colheita. Os extratos aquosos a 10 por cento e os decoctos (subprodutos da hidrodestilação) foram adicionados em BDA, autoclavados e distribuídos em placas de Petri. Os óleos essenciais foram adicionados em três pontos eqüidistantes nas placas de Petri contendo BDA. Discos dos isolados foram repicados para o centro das placas de Petri. O efeito fungitóxico foi avaliado medindo-se o diâmetro das colônias, quando na testemunha ou em qualquer tratamento os patógenos atingiram a borda da placa. O extrato aquoso e o óleo essencial de cravo-da-Índia inibiram em 100 por cento o crescimento de G. cingulata e C. gloeosporioides, sendo este último totalmente inibido pelo óleo essencial de capim-limão. Os decoctos de alecrim, gengibre, calêndula e laranja (Citrus sinensis) apresentaram potencial de inibição sobre os isolados dos patógenos. No controle de C. gloeosporioides, destacaram-se também os decoctos de marcela, camomila e tagetes. A inibição total ou parcial do crescimento micelial de Glomerella cingulata e Colletotrichum gloeosporioides, in vitro, evidenciou a existência de compostos biologicamente ativos, com efeito fungitóxico nos extratos, decoctos e óleos essenciais de plantas medicinais e aromáticas. Isto indica uma aplicação potencial destes produtos no controle alternativo da antracnose em frutos de goiabeira.

The main disease of the guava (Psidium guajava L.), after harvesting, is anthracnose, caused by Glomerella cingulata and Colletotrichum gloeosporioides. This pathogen and chemical residues of fungicides in fruits are considered the mains problems for exportation. In this work was evaluated the fungitoxic effect of extracts, decoctions and essential oils of medicinal and aromatic plants in the mycelium growth of the pathogens, in vitro, to be recommended as an alternative to chemical control in postharvest. To prepare the aquous extracts a 10 percent and the decoctions (sub-products of hydrodistillation) were added in PDA medium, autoclaved and distributed into Petri dishes. The essential oils were added in three equidistant points by Petri dishes contening PDA medium. Discs of the isolates of the pathogen were placed on the center of the Petri dishes. The fungitoxic effect was evaluated by measuring the diameter of the colonies when the pathogens or any other treatment reached the border of the Petri dishes. The aquous extract and the essential oil from the clove inhibit in 100 percent of G. cingulata and C. gloeosporioides growth, and the last one was totally inhibited by the essential oil of the lemon grass. The decoctions of rosemary, ginger, calendula, orange (Citrus sinensis) showed inhibition potencial to control both isolates of the pathogen. In the control of C. gloeosporioides also had good results the decoctions of mayweed, chamomile and tagetes. The total or partial mycelium growth inhibition of the Glomerella cingulata e Colletotrichum gloeosporioides showed the existence of compounds biologically actives with fungitoxic effect, in the extracts, decoctions and essential oils of medicinal and aromatic plants. This indicated a potential application of this products as an alternative to control anthracnose in guavas fruits.

Metodologia para detecção de infecções latentes de Monilinia fructicola em frutas de caroço / Methodology for the detection of latent infections of Monilinia fructicola in stone fruits

O fungo Monilinia fructicola, causador da podridão parda, é o patógeno mais importante economicamente para as fruteiras de caroço. O fungo infecta desde a fase de floração, podendo ficar latente nos frutos e manifestar sintomas durante a colheita e pós-colheita. O presente trabalho objetivou avaliar metodologias para detectar infecções latentes em frutos em desenvolvimento para diferentes cultivares de fruteiras de caroço. Foram escolhidas oito cultivares de fruteiras de caroço para avaliar infecções latentes de M. fructicola. Para tanto, coletaram-se 30 frutos de cada cultivar, separando-os em dois lotes de 15 frutos, sendo um deles submetido à imersão, por dois minutos, em solução de etanol 70 por cento, posteriormente em hipoclorito de sódio 2 por cento, e finalmente lavados em água esterilizada. O tratamento para o segundo lote de frutos foi semelhante ao anterior, acrescido de solução de paraquat 2 por cento (paraquat - 200g L-1, Gramocil- SC) após o hipoclorito. Após os tratamentos, os frutos foram postos em câmara úmida em embalagens plásticas contendo papel de filtro umedecido, à temperatura ambiente. A avaliação da incidência da doença foi feita a cada 48 horas por um período de 10 dias. As infecções latentes foram detectadas na maioria das cultivares avaliadas com os dois métodos, sendo que, nos frutos próximo à maturação, estas surgiram com maior freqüência. O tratamento em que se usou o herbicida aumentou a detecção da podridão parda nas cultivares de ameixa Reubennel e Harry Pickstone. Para os pêssegos, na cultivar "BR-3", os dois métodos favoreceram a detecção, porém, nas cultivares "Chimarrita" e "Vila Nova", quando não se utilizou o paraquat, houve maior detecção das infecções latentes.

The brown rot fungus Monilinia fructicola is economically the most important pathogen for stone fruit trees. The fungus infects since blossoming and may remain latent in the fruits and manifest symptoms during harvest and postharvest. The present study aimed at evaluating methodologies to detect latent infections in developing fruits for different cultivars of stone fruit trees. Eight cultivars of stone fruit trees were chosen in order to evaluate latent infections of M. fructicola. For that purpose, 30 fruits of each cultivar were collected and separated into two batches of 15 fruits. One of them was subject to immersion for two minutes in a sequence of ethanol solution 70 percent, later, sodium hypochlorite 2 percent, and finally washed in sterilized water. The treatment for the second batch of fruits was similar to the first one, adding paraquat solution 2 percent (paraquat - 200g L-1, Gramocil-SC) after the hypochlorite. After the treatments, the fruits were laid in humid chamber in plastic packages containing humid filter paper at room temperature. The evaluation of the incidence of the disease was carried out every 48 hours for 10 days. The latent infections were detected in the majority of the cultivars evaluated with the two methods, mainly in the fruits closer to maturation. The treatment in which herbicide was used increased the detection of brown rot in the plum cultivars Reubennel and Harry Pickstone. For the peaches in cultivar BR-3, the two methods favored the detection; however, in the cultivars Chimarrita and Vila Nova there was higher detection of latent infection when paraquat was not utilized.

Qualidade de frutos de caqui 'Jiro' ensacados com diferentes embalagens / Fruit quality of 'Jiro' japanese persimmon fruits bagged with different types of packings

A técnica de ensacamento de frutos vem sendo utilizadas por muitos produtores, visando a melhorqualidade de frutos, redução de aplicação de agrotóxicos e diminuição dos danos provocados porpragas e doenças. O presente trabalho avaliou a qualidade de frutos de caquizeiro 'Jiro' ensacados comdiferentes tipos de embalagens. O experimento foi conduzido no pomar de caquizeiro da FazendaExperimental do Canguiri da UFPR, localizada em Pinhais-PR. A adubação e o manejo do pomar foirealizado de forma orgânica. Os tratamentos foram: saco plástico microperfurado, saco de papel pardo,saco de papel manteiga, saco de jornal e testemunha sem ensacamento. O delineamento experimental foiem blocos ao acaso com cinco repetições, sendo cada planta considerada um bloco. Cada parcela foicomposta de 20 frutos. O ensacamento foi realizado após a queda fisiológica dos frutos jovens,procurando-se distribuir os tipos de sacos aleatoriamente dentro de cada planta. A avaliação foi realizadaapós 77 dias do ensacamento, verificando-se o número de frutos colhidos por parcela, massa dos frutos,diâmetro dos frutos, número de frutos em cada categoria de maturação, número de frutos infectados porsujeira-de-mosca (Schyzothyrium pomi) e teor de sólidos solúveis. Houve diferença entre os tratamentosapenas na incidência de sujeira-de-mosca e na porcentagem de coloração dos frutos. O ensacamento decaqui com sacos de jornal ou papel pardo reduziu a incidência do fungo S. pomi e a coloração daepiderme dos frutos. O tamanho e teor de sólidos solúveis não foram influenciados pelo ensacamento.

The bagging technique of fruits comes being used by many producers, aiming at the fruit quality,reduction of application of agro toxics and reduction of the damages caused by insects and diseases.The present work evaluated the quality of bagging fruits of 'Jiro' japanese persimmon with differenttypes of bags. The experiment was lead in the orchard of Japanese persimmon of the Experimental Farmof the Canguiri of the UFPR, located in Pinhais-PR. The fertilization and the management of the orchardwere entirely organic. The treatments had been: microperforated plastic bag, brown kraft paper bag,butter-like paper bag, newspaper bag and without bagging. The experimental design used was randomizedblocks with five replicates, being each plant considered a block. Each parcel was composed by 20 fruits.The bagging was made after the physiological fall of the young fruits, distributing the types of bags aleatorely inside each plant. The evaluation was 77 days after bagging, verifying the number of fruitsharvested, mass of the fruits, diameter of the fruits, number of fruits in each category of maturation,number of fruits attacked with Schyzothyrium pomi and soluble solids content. The results were significantonly in the incidence of S. pomi and in the percentage of coloration of the fruits. The bagging withnewspaper and brown kraft bags reduced the incidence of S. Pomi and the color of fruits. The size andthe soluble solids content were not affected by bagging