RESUMO
Interduplex crosslinks by a bifunctional anthramycin DNA crosslinker produced triradial and quadriradial chromosomes. The crosslinker alkylates guanine at N-2. Bovine chromosomes contain GC-rich density satellite DNAs at the centromeric heterochromatin and is the basis for the formation of triradial and quadriradial chromosomes at the centromeres. The in situ crosslinking of interphase chromosomes indicates that the distance between centromeres is 17.5 A. We conclude that the nuclear matrix associated DNA in the centromeric heterochromatin of interphase chromosomes are positioned close enough for crosslinking to occur. We propose a model for the generation of triradial and quadriradial chromosomes based upon the number of interduplex crosslinks between two chromosomes.
Assuntos
Antramicina/farmacologia , Aberrações Cromossômicas , Reagentes de Ligações Cruzadas/farmacologia , DNA Satélite/efeitos dos fármacos , Animais , Bovinos , Linhagem Celular , Hibridização in Situ Fluorescente , CariotipagemRESUMO
A Massachusetts hospital with a significant geriatric population initiated a pilot program to reduce use of restraints on an inpatient medical-surgical unit. A task force developed and implemented a successful program that included identification of alternatives to restraint use, policy and procedure development, staff education and evaluation.
