Assuntos
Derivados de Benzeno/química , Materiais Biomiméticos/síntese química , Hidrogéis/síntese química , Nanofibras/química , Polietilenoglicóis/química , Tensoativos/química , Simulação por Computador , Cristalização/métodos , Substâncias Macromoleculares/química , Teste de Materiais , Modelos Químicos , Nanofibras/ultraestrutura , Tamanho da PartículaRESUMO
Because of the rapidly growing field of nanoparticles in therapeutic applications, understanding and controlling the interaction between nanoparticles and membranes is of great importance. While a membrane is exposed to nanoparticles its behavior is mediated by both their biological and physical properties. Constant interplay of these biological and physicochemical factors makes selective studies of nanoparticles uptake demanding. Artificial model membranes can serve as a platform to investigate physical parameters of the process in the absence of any biofunctional molecules and/or supplementary energy. Here we report on photon- and fluorescence-correlation spectroscopic studies of the uptake of nanosized SiO(2) nanoparticles by poly(dimethylsiloxane)-block-poly(2-methyloxazoline) vesicles allowing species selectivity. Analogous to the cell membrane, polymeric membrane incorporates particles using membrane fission and particles wrapping as suggested by cryo-TEM imaging. It is revealed that the incorporation process can be controlled to a significant extent by changing nanoparticles size and concentration. Conditions for nanoparticle uptake and controlled filling of polymersomes are presented.
Assuntos
Cristalização/métodos , Nanoestruturas/química , Nanoestruturas/ultraestrutura , Dióxido de Silício/química , Adsorção , Substâncias Macromoleculares/química , Teste de Materiais , Conformação Molecular , Tamanho da Partícula , Propriedades de SuperfícieRESUMO
A modular one-component supramolecular transient network in water, based on poly(ethylene glycol) and end-capped with four-fold hydrogen bonding units, is reported. Due to its nonlinear structural formation, this system allows active proteins to be added to the hydrogel during formation. Once implanted in vivo it releases the protein by erosion of both the protein and polymer via dissolution.
