Development and validation of a stability-indicating high performance liquid chromatographic assay for determination of cariprazine in bulk form and in drug product.

The aim of the present study is to develop a stability indicating high performance liquid chromatographic method for the determination of cariprazine in bulk substance and in drug product. The chromatographic separation was carried out using a Phenomenex Kinetex® C18 column (5µm, 250×4.6mm) and a mobile phase consisting of acetonitrile-potassium dihydrogen orthophosphate buffer (pH 4; 50mM) (30:70, v/v), at a flow rate of 1mlmin -1 and UV detection at 248nm. The column was maintained at 25°C and an injection volume of 20µL was used. Stress testing of cariprazine bulk substance and drug product was performed according to the International Conference on Harmonization (ICH) Q1A (R2) guideline. Various stress conditions were tested including acidic, alkaline and neutral hydrolysis, humidity, oxidation, dry heat and photolysis. A total of three degradation products (DPs) were formed. Among them two DPs were successfully characterized with the liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis.

[Study on a focus of ascaridiasis in a locality of Cap Bon (Tunisia)]. / Etude d'un foyer d'ascaridiase dans une localité du Cap Bon (Tunisie)

The study of ascaridiasis in a single locality of the Cap Bon region (Tunisia) where prevalence of this helminthiasis is much higher than anywhere else in the region, shows that 71,1% of the population is infected and all ages approximately in the same proportion. This high prevalence can be explained by a cumulative effect of two transmission factors: a particular construction of houses, which have a kind of reservoir for organic wastes in direct connection with the street, and the use of human excrements in agriculture. Mass chemotherapy every 3 months during several consecutive years is proposed.