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1.
J Bacteriol ; 200(11)2018 06 01.
Artigo em Inglês | MEDLINE | ID: mdl-29555700

RESUMO

Bacterial pathogenesis depends on changes in metabolic and virulence gene expression in response to changes within a pathogen's environment. The plague-causing pathogen, Yersinia pestis, requires expression of the gene encoding the Pla protease for progression of pneumonic plague. The catabolite repressor protein Crp, a global transcriptional regulator, may serve as the activator of pla in response to changes within the lungs as disease progresses. By using gene reporter fusions, the spatial and temporal activation of the crp and pla promoters was measured in a mouse model of pneumonic plague. In the lungs, crp was highly expressed in bacteria found within large aggregates resembling biofilms, while pla expression increased over time independent of the aggregated state. Increased expression of crp and pla correlated with a reduction in lung glucose levels. Deletion of the glucose-specific phosphotransferase system EIIBC (PtsG) of Y. pestis rescued glucose levels in the lungs, resulting in reduced expression of both crp and pla We propose that activation of pla expression during pneumonic plague is driven by an increase of both Crp and cAMP levels following consumption of available glucose in the lungs by Y. pestis Thus, Crp operates as a sensor linking the nutritional environment of the host to regulation of virulence gene expression.IMPORTANCE Using Yersinia pestis as a model for pneumonia, we discovered that glucose is rapidly consumed, leading to a catabolite-repressive environment in the lungs. As a result, expression of the gene encoding the plasminogen activator protease, a target of the catabolite repressor protein required for Y. pestis pathogenesis, is activated. Interestingly, expression of the catabolite repressor protein itself was also increased in the absence of glucose but only in biofilms. The data presented here demonstrate how a bacterial pathogen senses changes within its environment to coordinate metabolism and virulence gene expression.


Assuntos
Proteínas de Bactérias/metabolismo , Repressão Catabólica , Glucose/deficiência , Peste/microbiologia , Yersinia pestis/metabolismo , Yersinia pestis/patogenicidade , Animais , Proteínas de Bactérias/genética , Modelos Animais de Doenças , Genes Reporter , Pulmão/microbiologia , Camundongos , Camundongos Endogâmicos C57BL , Sistema Fosfotransferase de Açúcar do Fosfoenolpiruvato/genética , Sistema Fosfotransferase de Açúcar do Fosfoenolpiruvato/metabolismo , Ativadores de Plasminogênio/genética , Ativadores de Plasminogênio/metabolismo , Regiões Promotoras Genéticas/genética , Virulência , Yersinia pestis/genética
2.
J Infect Dis ; 215(suppl_1): S37-S43, 2017 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-28375518

RESUMO

Klebsiella pneumoniae has a reputation for causing a wide range of infectious conditions, with numerous highly virulent and antibiotic-resistant strains. Metabolic models have the potential to provide insights into the growth behavior, nutrient requirements, essential genes, and candidate drug targets in these strains. Here we develop a metabolic model for KPPR1, a highly virulent strain of K. pneumoniae. We apply a combination of Biolog phenotype data and fitness data to validate and refine our KPPR1 model. The final model displays a predictive accuracy of 75% in identifying potential carbon and nitrogen sources for K. pneumoniae and of 99% in predicting nonessential genes in rich media. We demonstrate how this model is useful in studying the differences in the metabolic capabilities of the low-virulence MGH 78578 strain and the highly virulent KPPR1 strain. For example, we demonstrate that these strains differ in carbohydrate metabolism, including the ability to metabolize dulcitol as a primary carbon source. Our model makes numerous other predictions for follow-up verification and analysis.


Assuntos
Genoma Bacteriano , Klebsiella pneumoniae/metabolismo , Modelos Biológicos , Metabolismo dos Carboidratos , Meios de Cultura , Farmacorresistência Bacteriana Múltipla/genética , Genes Essenciais , Klebsiella pneumoniae/efeitos dos fármacos , Klebsiella pneumoniae/genética , Fenótipo , Análise de Sequência de DNA
3.
Genome Announc ; 4(3)2016 May 26.
Artigo em Inglês | MEDLINE | ID: mdl-27231362

RESUMO

We report here the draft genome sequence of a multidrug-resistant clinical isolate of Klebsiella quasipneumoniae subsp. similipneumoniae, KP_Z4175. This strain, isolated as part of a hospital infection-control screening program, is resistant to multiple ß-lactam antibiotics, aminoglycosides, and trimethoprim-sulfamethoxazole.

4.
Infect Immun ; 84(1): 365-74, 2016 01.
Artigo em Inglês | MEDLINE | ID: mdl-26553463

RESUMO

Pneumonic plague represents the most severe form of disease caused by Yersinia pestis due to its ease of transmission, rapid progression, and high mortality rate. The Y. pestis outer membrane Pla protease is essential for the development of pneumonic plague; however, the complete repertoire of substrates cleaved by Pla in the lungs is not known. In this study, we describe a proteomic screen to identify host proteins contained within the bronchoalveolar lavage fluid of mice that are cleaved and/or processed by Y. pestis in a Pla-dependent manner. We identified peroxiredoxin 6 (Prdx6), a host factor that contributes to pulmonary surfactant metabolism and lung defense against oxidative stress, as a previously unknown substrate of Pla. Pla cleaves Prdx6 at three distinct sites, and these cleavages disrupt both the peroxidase and phospholipase A2 activities of Prdx6. In addition, we found that infection with wild-type Y. pestis reduces the abundance of extracellular Prdx6 in the lungs compared to that after infection with Δpla Y. pestis, suggesting that Pla cleaves Prdx6 in the pulmonary compartment. However, following infection with either wild-type or Δpla Y. pestis, Prdx6-deficient mice exhibit no differences in bacterial burden, host immune response, or lung damage from wild-type mice. Thus, while Pla is able to disrupt Prdx6 function in vitro and reduce Prdx6 levels in vivo, the cleavage of Prdx6 has little detectable impact on the progression or outcome of pneumonic plague.


Assuntos
Proteínas da Membrana Bacteriana Externa/metabolismo , Proteínas de Bactérias/metabolismo , Peroxirredoxina VI/metabolismo , Ativadores de Plasminogênio/metabolismo , Yersinia pestis/metabolismo , Animais , Proteínas de Bactérias/genética , Líquido da Lavagem Broncoalveolar/química , Progressão da Doença , Feminino , Pulmão/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Peroxidase/metabolismo , Fosfolipases A2/metabolismo , Peste/microbiologia , Ativadores de Plasminogênio/genética , Yersinia pestis/genética , Yersinia pestis/imunologia
5.
Infect Immun ; 83(12): 4837-47, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26438794

RESUMO

Many pathogens usurp the host hemostatic system during infection to promote pathogenesis. Yersinia pestis, the causative agent of plague, expresses the plasminogen activator protease Pla, which has been shown in vitro to target and cleave multiple proteins within the fibrinolytic pathway, including the plasmin inhibitor α2-antiplasmin (A2AP). It is not known, however, if Pla inactivates A2AP in vivo; the role of A2AP during respiratory Y. pestis infection is not known either. Here, we show that Y. pestis does not appreciably cleave A2AP in a Pla-dependent manner in the lungs during experimental pneumonic plague. Furthermore, following intranasal infection with Y. pestis, A2AP-deficient mice exhibit no difference in survival time, bacterial burden in the lungs, or dissemination from wild-type mice. Instead, we found that in the absence of Pla, A2AP contributes to the control of the pulmonary inflammatory response during infection by reducing neutrophil recruitment and cytokine production, resulting in altered immunopathology of the lungs compared to A2AP-deficient mice. Thus, our data demonstrate that A2AP is not significantly affected by the Pla protease during pneumonic plague, and although A2AP participates in immune modulation in the lungs, it has limited impact on the course or ultimate outcome of the infection.


Assuntos
Proteínas de Bactérias/imunologia , Regulação Bacteriana da Expressão Gênica , Pulmão/imunologia , Peste/imunologia , Ativadores de Plasminogênio/imunologia , Inibidores de Serina Proteinase/imunologia , Yersinia pestis/imunologia , alfa 2-Antiplasmina/imunologia , Animais , Carga Bacteriana , Proteínas de Bactérias/genética , Líquido da Lavagem Broncoalveolar/química , Líquido da Lavagem Broncoalveolar/citologia , Líquido da Lavagem Broncoalveolar/imunologia , Progressão da Doença , Interações Hospedeiro-Patógeno , Imunidade Inata , Pulmão/microbiologia , Pulmão/patologia , Camundongos , Camundongos Endogâmicos C57BL , Neutrófilos/imunologia , Neutrófilos/microbiologia , Neutrófilos/patologia , Peste/microbiologia , Peste/mortalidade , Peste/patologia , Ativadores de Plasminogênio/genética , Inibidores de Serina Proteinase/genética , Transdução de Sinais , Análise de Sobrevida , Yersinia pestis/genética , Yersinia pestis/patogenicidade , alfa 2-Antiplasmina/deficiência , alfa 2-Antiplasmina/genética
6.
Nat Commun ; 6: 7487, 2015 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-26123398

RESUMO

Yersinia pestis causes the fatal respiratory disease pneumonic plague. Y. pestis recently evolved from the gastrointestinal pathogen Y. pseudotuberculosis; however, it is not known at what point Y. pestis gained the ability to induce a fulminant pneumonia. Here we show that the acquisition of a single gene encoding the protease Pla was sufficient for the most ancestral, deeply rooted strains of Y. pestis to cause pneumonic plague, indicating that Y. pestis was primed to infect the lungs at a very early stage in its evolution. As Y. pestis further evolved, modern strains acquired a single amino-acid modification within Pla that optimizes protease activity. While this modification is unnecessary to cause pneumonic plague, the substitution is instead needed to efficiently induce the invasive infection associated with bubonic plague. These findings indicate that Y. pestis was capable of causing pneumonic plague before it evolved to optimally cause invasive infections in mammals.


Assuntos
Peste/microbiologia , Yersinia pestis/classificação , Yersinia pestis/genética , Animais , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Feminino , Regulação Bacteriana da Expressão Gênica/fisiologia , Camundongos , Camundongos Endogâmicos C57BL , Yersinia pestis/patogenicidade
7.
PLoS One ; 9(9): e107002, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25198697

RESUMO

Many Gram-negative bacteria produce outer membrane vesicles (OMVs) during cell growth and division, and some bacterial pathogens deliver virulence factors to the host via the release of OMVs during infection. Here we show that Yersinia pestis, the causative agent of the disease plague, produces and releases native OMVs under physiological conditions. These OMVs, approximately 100 nm in diameter, contain multiple virulence-associated outer membrane proteins including the adhesin Ail, the F1 outer fimbrial antigen, and the protease Pla. We found that OMVs released by Y. pestis contain catalytically active Pla that is competent for plasminogen activation and α2-antiplasmin degradation. The abundance of OMV-associated proteins released by Y. pestis is significantly elevated at 37 °C compared to 26 °C and is increased in response to membrane stress and mutations in RseA, Hfq, and the major Braun lipoprotein (Lpp). In addition, we show that Y. pestis OMVs are able to bind to components of the extracellular matrix such as fibronectin and laminin. These data suggest that Y. pestis may produce OMVs during mammalian infection and we propose that dispersal of Pla via OMV release may influence the outcome of infection through interactions with Pla substrates such as plasminogen and Fas ligand.


Assuntos
Proteínas da Membrana Bacteriana Externa/metabolismo , Proteínas de Bactérias/metabolismo , Peste/microbiologia , Vesículas Secretórias/metabolismo , Fatores de Virulência/metabolismo , Yersinia pestis/patogenicidade , Cromatografia Líquida , Fibronectinas/metabolismo , Humanos , Laminina/metabolismo , Ativadores de Plasminogênio/metabolismo , Proteômica , Espectrometria de Massas em Tandem , Virulência
9.
Cell Host Microbe ; 15(4): 424-34, 2014 Apr 09.
Artigo em Inglês | MEDLINE | ID: mdl-24721571

RESUMO

Pneumonic plague is a deadly respiratory disease caused by Yersinia pestis. The bacterial protease Pla contributes to disease progression and manipulation of host immunity, but the mechanisms by which this occurs are largely unknown. Here we show that Pla degrades the apoptotic signaling molecule Fas ligand (FasL) to prevent host cell apoptosis and inflammation. Wild-type Y. pestis, but not a Pla mutant (Δpla), degrades FasL, which results in decreased downstream caspase-3/7 activation and reduced apoptosis. Similarly, lungs of mice challenged with wild-type Y. pestis show reduced levels of FasL and activated caspase-3/7 compared to Δpla infection. Consistent with a role for FasL in regulating immune responses, Δpla infection results in aberrant proinflammatory cytokine levels. The loss of FasL or inhibition of caspase activity alters host inflammatory responses and enables enhanced Y. pestis outgrowth in the lungs. Thus, by degrading FasL, Y. pestis manipulates host cell death pathways to facilitate infection.


Assuntos
Proteínas de Bactérias/metabolismo , Caspase 3/biossíntese , Caspase 7/biossíntese , Proteína Ligante Fas/metabolismo , Ativadores de Plasminogênio/metabolismo , Yersinia pestis/patogenicidade , Animais , Apoptose , Proteínas de Bactérias/genética , Linhagem Celular Tumoral , Progressão da Doença , Proteína Ligante Fas/biossíntese , Proteína Ligante Fas/genética , Humanos , Inflamação , Células Jurkat , Pulmão/patologia , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Peste/patologia , Ativadores de Plasminogênio/genética , Yersinia pestis/genética
10.
J Bacteriol ; 196(9): 1659-70, 2014 May.
Artigo em Inglês | MEDLINE | ID: mdl-24532772

RESUMO

Small noncoding RNA (sRNA) molecules are integral components of the regulatory machinery for many bacterial species and are known to posttranscriptionally regulate metabolic and stress-response pathways, quorum sensing, virulence factors, and more. The Yop-Ysc type III secretion system (T3SS) is a critical virulence component for the pathogenic Yersinia species, and the regulation of this system is tightly controlled at each step from transcription to translocation of effectors into host cells. The contribution of sRNAs to the regulation of the T3SS in Yersinia has been largely unstudied, however. Previously, our lab identified a role for the sRNA chaperone protein Hfq in the regulation of components of the T3SS in the gastrointestinal pathogen Yersinia pseudotuberculosis. Here we present data demonstrating a similar requirement for Hfq in the closely related species Yersinia pestis. Through deep sequencing analysis of the Y. pestis sRNA-ome, we found 63 previously unidentified putative sRNAs in this species. We identified a Yersinia-specific sRNA, Ysr141, carried by the T3SS plasmid pCD1 that is required for the production of multiple T3SS proteins. In addition, we show that Ysr141 targets an untranslated region upstream of yopJ to posttranscriptionally activate the synthesis of the YopJ protein. Furthermore, Ysr141 may be an unstable and/or processed sRNA, which could contribute to its function in the regulation of the T3SS. The discovery of an sRNA that influences the synthesis of the T3SS adds an additional layer of regulation to this tightly controlled virulence determinant of Y. pestis.


Assuntos
Proteínas de Bactérias/genética , Sistemas de Secreção Bacterianos , Regulação Bacteriana da Expressão Gênica , Genoma Bacteriano , RNA Bacteriano/genética , Pequeno RNA não Traduzido/genética , Yersinia pestis/genética , Proteínas de Bactérias/metabolismo , RNA Bacteriano/metabolismo , Pequeno RNA não Traduzido/metabolismo , Yersinia pestis/metabolismo
11.
mBio ; 5(1): e01038-13, 2014 Feb 11.
Artigo em Inglês | MEDLINE | ID: mdl-24520064

RESUMO

UNLABELLED: The cyclic AMP receptor protein (Crp) is a transcriptional regulator that controls the expression of numerous bacterial genes, usually in response to environmental conditions and particularly by sensing the availability of carbon. In the plague pathogen Yersinia pestis, Crp regulates the expression of multiple virulence factors, including components of the type III secretion system and the plasminogen activator protease Pla. The regulation of Crp itself, however, is distinctly different from that found in the well-studied Escherichia coli system. Here, we show that at physiological temperatures, the synthesis of Crp in Y. pestis is positively regulated at the posttranscriptional level. The loss of the small RNA chaperone Hfq results in decreased Crp protein levels but not in steady-state Crp transcript levels, and this regulatory effect occurs within the 5' untranslated region (UTR) of the Crp mRNA. The posttranscriptional activation of Crp synthesis is required for the expression of pla, and decoupling crp from Hfq through the use of an exogenously controlled promoter and 5' UTR increases Pla protein levels as well as partially rescues the growth defect associated with the loss of Hfq. Finally, we show that both Hfq and the posttranscriptional regulation of Crp contribute to the virulence of Y. pestis during pneumonic plague. The Hfq-dependent, posttranscriptional regulation of Crp may be specific to Yersinia species, and thus our data help explain the dramatic growth and virulence defects associated with the loss of Hfq in Y. pestis. IMPORTANCE: The Crp protein is a major transcriptional regulator in bacteria, and its synthesis is tightly controlled to avoid inappropriate induction of the Crp regulon. In this report, we provide the first evidence of Crp regulation in an Hfq-dependent manner at the posttranscriptional level. Our discovery that the synthesis of Crp in Yersinia pestis is Hfq dependent adds an additional layer of regulation to catabolite repression in this bacterium. Our work provides a mechanism by which the plague pathogen links not just the sensing of glucose or other carbon sources but also other signals that influence Crp abundance via the expression of small RNAs to the induction of the Crp regulon. In turn, this allows Y. pestis to fine-tune Crp levels to optimize virulence gene expression during plague infection and may allow the bacterium to adapt to its unique environmental niches.


Assuntos
Proteína Receptora de AMP Cíclico/biossíntese , Regulação Bacteriana da Expressão Gênica , Yersinia pestis/genética , Yersinia pestis/patogenicidade , Animais , Modelos Animais de Doenças , Feminino , Camundongos , Camundongos Endogâmicos C57BL , Peste/microbiologia , Peste/patologia , Temperatura , Virulência
12.
Infect Immun ; 81(4): 1186-97, 2013 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-23357388

RESUMO

Yersinia pestis, the causative agent of plague, uses a type III secretion system (T3SS) to inject cytotoxic Yop proteins directly into the cytosol of mammalian host cells. The T3SS can also be activated in vitro at 37°C in the absence of calcium. The chromosomal gene rfaL (waaL) was recently identified as a virulence factor required for proper function of the T3SS. RfaL functions as a ligase that adds the terminal N-acetylglucosamine to the lipooligosaccharide core of Y. pestis. We previously showed that deletion of rfaL prevents secretion of Yops in vitro. Here we show that the divalent cations calcium, strontium, and magnesium can partially or fully rescue Yop secretion in vitro, indicating that the secretion phenotype of the rfaL mutant may be due to structural changes in the outer membrane and the corresponding feedback inhibition on the T3SS. In support of this, we found that the defect can be overcome by deleting the regulatory gene lcrQ. Consistent with a defective T3SS, the rfaL mutant is less virulent than the wild type. We show here that the virulence defect of the mutant correlates with a decrease in both T3SS gene expression and ability to inject innate immune cells, combined with an increased sensitivity to cationic antimicrobial peptides.


Assuntos
Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Sistemas de Secreção Bacterianos , Fatores de Virulência/genética , Fatores de Virulência/metabolismo , Yersinia pestis/genética , Yersinia pestis/patogenicidade , Animais , Carga Bacteriana , Cátions Bivalentes/metabolismo , Modelos Animais de Doenças , Feminino , Deleção de Genes , Ligases/genética , Ligases/metabolismo , Proteínas de Membrana Transportadoras/genética , Proteínas de Membrana Transportadoras/metabolismo , Metais/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Peste/microbiologia , Peste/patologia , Baço/microbiologia , Virulência
13.
Artigo em Inglês | MEDLINE | ID: mdl-23162797

RESUMO

Proper regulation of gene expression is required by bacterial pathogens to respond to continually changing environmental conditions and the host response during the infectious process. While transcriptional regulation is perhaps the most well understood form of controlling gene expression, recent studies have demonstrated the importance of post-transcriptional mechanisms of gene regulation that allow for more refined management of the bacterial response to host conditions. Yersinia species of bacteria are known to use various forms of post-transcriptional regulation for control of many virulence-associated genes. These include regulation by cis- and trans-acting small non-coding RNAs, RNA-binding proteins, RNases, and thermoswitches. The effects of these and other regulatory mechanisms on Yersinia physiology can be profound and have been shown to influence type III secretion, motility, biofilm formation, host cell invasion, intracellular survival and replication, and more. In this review, we discuss these and other post-transcriptional mechanisms and their influence on virulence gene regulation, with a particular emphasis on how these processes influence the virulence of Yersinia in the host.


Assuntos
Regulação Bacteriana da Expressão Gênica , Fatores de Virulência/biossíntese , Yersinia/genética , Humanos , Conformação de Ácido Nucleico , Processamento Pós-Transcricional do RNA , RNA Interferente Pequeno/metabolismo , Proteínas de Ligação a RNA/metabolismo , Fatores de Virulência/genética , Yersinia/patogenicidade , Yersinia/fisiologia
14.
Mol Microbiol ; 86(3): 661-74, 2012 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-22924957

RESUMO

Yersinia pestis, the cause of the disease plague, forms biofilms to enhance flea-to-mammal transmission. Biofilm formation is dependent on exopolysaccharide synthesis and is controlled by the intracellular levels of the second messenger molecule cyclic diguanylate (c-di-GMP), but the mechanisms by which Y. pestis regulates c-di-GMP synthesis and turnover are not fully understood. Here we show that the small RNA chaperone Hfq contributes to the regulation of c-di-GMP levels and biofilm formation by modulating the abundance of both the c-di-GMP phosphodiesterase HmsP and the diguanylate cyclase HmsT. To do so, Hfq co-ordinately promotes hmsP mRNA accumulation while simultaneously decreasing the stability of the hmsT transcript. Hfq-dependent regulation of HmsP occurs at the transcriptional level while the regulation of HmsT is post-transcriptional and is localized to the 5' untranslated region/proximal coding sequence of the hmsT transcript. Decoupling HmsP from Hfq-based regulation is sufficient to overcome the effects of Δhfq on c-di-GMP and biofilm formation. We propose that Y. pestis utilizes Hfq to link c-di-GMP levels to environmental conditions and that the disregulation of c-di-GMP turnover in the absence of Hfq may contribute to the severe attenuation of Y. pestis lacking this RNA chaperone in animal models of plague.


Assuntos
Proteínas de Bactérias/metabolismo , GMP Cíclico/análogos & derivados , Regulação Bacteriana da Expressão Gênica , Fator Proteico 1 do Hospedeiro/metabolismo , Peste/microbiologia , Yersinia pestis/metabolismo , 3',5'-GMP Cíclico Fosfodiesterases/genética , 3',5'-GMP Cíclico Fosfodiesterases/metabolismo , Proteínas de Bactérias/genética , Sequência de Bases , GMP Cíclico/biossíntese , Proteínas de Escherichia coli/genética , Proteínas de Escherichia coli/metabolismo , Fator Proteico 1 do Hospedeiro/genética , Humanos , Dados de Sequência Molecular , Fósforo-Oxigênio Liases/genética , Fósforo-Oxigênio Liases/metabolismo , Yersinia pestis/enzimologia , Yersinia pestis/genética
18.
Proc Natl Acad Sci U S A ; 108(37): E709-17, 2011 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-21876162

RESUMO

A major class of bacterial small, noncoding RNAs (sRNAs) acts by base-pairing with mRNAs to alter the translation from and/or stability of the transcript. Our laboratory has shown that Hfq, the chaperone that mediates the interaction of many sRNAs with their targets, is required for the virulence of the enteropathogen Yersinia pseudotuberculosis. This finding suggests that sRNAs play a critical role in the regulation of virulence in this pathogen, but these sRNAs are not known. Using a deep sequencing approach, we identified the global set of sRNAs expressed in vitro by Y. pseudotuberculosis. Sequencing of RNA libraries from bacteria grown at 26 °C and 37 °C resulted in the identification of 150 unannotated sRNAs. The majority of these sRNAs are Yersinia specific, without orthologs in either Escherichia coli or Salmonella typhimurium. Six sRNAs are Y. pseudotuberculosis specific and are absent from the genome of the closely related species Yersinia pestis. We found that the expression of many sRNAs conserved between Y. pseudotuberculosis and Y. pestis differs in both timing and dependence on Hfq, suggesting evolutionary changes in posttranscriptional regulation between these species. Deletion of multiple sRNAs in Y. pseudotuberculosis leads to attenuation of the pathogen in a mouse model of yersiniosis, as does the inactivation in Y. pestis of a conserved, Yersinia-specific sRNA in a mouse model of pneumonic plague. Finally, we determined the regulon controlled by one of these sRNAs, revealing potential virulence determinants in Y. pseudotuberculosis that are regulated in a posttranscriptional manner.


Assuntos
RNA Bacteriano/genética , RNA não Traduzido/genética , Yersinia pseudotuberculosis/genética , Yersinia pseudotuberculosis/patogenicidade , Animais , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Sequência de Bases , Regulação Bacteriana da Expressão Gênica , Camundongos , Anotação de Sequência Molecular , Dados de Sequência Molecular , RNA Bacteriano/metabolismo , RNA não Traduzido/metabolismo , Reprodutibilidade dos Testes , Especificidade da Espécie , Transcrição Gênica , Virulência/genética , Yersinia pestis/genética , Yersinia pestis/patogenicidade , Infecções por Yersinia pseudotuberculosis/genética , Infecções por Yersinia pseudotuberculosis/microbiologia
19.
Infect Immun ; 78(5): 2034-44, 2010 May.
Artigo em Inglês | MEDLINE | ID: mdl-20231416

RESUMO

Bacterial small, noncoding RNAs (sRNAs) participate in the posttranscriptional regulation of gene expression, often by affecting protein translation, transcript stability, and/or protein activity. For proper function, many sRNAs rely on the chaperone Hfq, which mediates the interaction of the sRNA with its target mRNA. Recent studies have demonstrated that Hfq contributes to the pathogenesis of a number of bacterial species, suggesting that sRNAs play an essential role in the regulation of virulence. The enteric pathogen Yersinia pseudotuberculosis causes the disease yersiniosis. Here we show that Hfq is required by Y. pseudotuberculosis to cause mortality in an intragastric mouse model of infection, and a strain lacking Hfq is attenuated 1,000-fold compared to the wild type. Hfq is also required for virulence through the intraperitoneal route of infection and for persistence of the bacterium in the Peyer's patches, mesenteric lymph nodes, and spleen, suggesting a role for Hfq in systemic infection. Furthermore, the Deltahfq mutant of Y. pseudotuberculosis is hypermotile and displays increased production of a biosurfactant-like substance, reduced intracellular survival in macrophages, and decreased production of type III secretion effector proteins. Together, these data demonstrate that Hfq plays a critical role in the virulence of Y. pseudotuberculosis by participating in the regulation of multiple steps in the pathogenic process and further highlight the unique role of Hfq in the virulence of individual pathogens.


Assuntos
Fator Proteico 1 do Hospedeiro/fisiologia , RNA Bacteriano/metabolismo , RNA Interferente Pequeno/metabolismo , Yersinia pseudotuberculosis/patogenicidade , Animais , Peso Corporal , Contagem de Colônia Microbiana , Feminino , Deleção de Genes , Fator Proteico 1 do Hospedeiro/genética , Intestino Delgado/microbiologia , Linfonodos/microbiologia , Camundongos , Camundongos Endogâmicos BALB C , Nódulos Linfáticos Agregados/microbiologia , Baço/microbiologia , Análise de Sobrevida , Virulência , Infecções por Yersinia pseudotuberculosis/microbiologia , Infecções por Yersinia pseudotuberculosis/patologia
20.
Science ; 315(5811): 509-13, 2007 Jan 26.
Artigo em Inglês | MEDLINE | ID: mdl-17255510

RESUMO

Primary pneumonic plague is transmitted easily, progresses rapidly, and causes high mortality, but the mechanisms by which Yersinia pestis overwhelms the lungs are largely unknown. We show that the plasminogen activator Pla is essential for Y. pestis to cause primary pneumonic plague but is less important for dissemination during pneumonic plague than during bubonic plague. Experiments manipulating its temporal expression showed that Pla allows Y. pestis to replicate rapidly in the airways, causing a lethal fulminant pneumonia; if unexpressed, inflammation is aborted, and lung repair is activated. Inhibition of Pla expression prolonged the survival of animals with the disease, offering a therapeutic option to extend the period during which antibiotics are effective.


Assuntos
Pulmão/microbiologia , Peste/microbiologia , Ativadores de Plasminogênio/metabolismo , Pneumonia Bacteriana/microbiologia , Yersinia pestis/patogenicidade , Animais , Proliferação de Células , Contagem de Colônia Microbiana , Citocinas/genética , Citocinas/metabolismo , Feminino , Fibrinogênio/metabolismo , Regulação da Expressão Gênica , Regulação Bacteriana da Expressão Gênica , Pulmão/imunologia , Pulmão/patologia , Camundongos , Camundongos Endogâmicos C57BL , Mutação , Peste/imunologia , Peste/patologia , Plasminogênio/metabolismo , Ativadores de Plasminogênio/genética , Pneumonia Bacteriana/imunologia , Pneumonia Bacteriana/patologia , Baço/microbiologia , Tetraciclinas/farmacologia , Fatores de Virulência/genética , Fatores de Virulência/metabolismo , Yersinia pestis/enzimologia , Yersinia pestis/genética , Yersinia pestis/crescimento & desenvolvimento
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