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J Dent Res ; 91(7): 683-9, 2012 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-22622662

RESUMO

Muenke syndrome is characterized by various craniofacial deformities and is caused by an autosomal-dominant activating mutation in fibroblast growth factor receptor 3 (FGFR3(P250R) ). Here, using mice carrying a corresponding mutation (FgfR3(P244R) ), we determined whether the mutation affects temporomandibular joint (TMJ) development and growth. In situ hybridization showed that FgfR3 was expressed in condylar chondroprogenitors and maturing chondrocytes that also expressed the Indian hedgehog (Ihh) receptor and transcriptional target Patched 1(Ptch1). In FgfR3(P244R) mutants, the condyles displayed reduced levels of Ihh expression, H4C-positive proliferating chondroprogenitors, and collagen type II- and type X-expressing chondrocytes. Primary bone spongiosa formation was also disturbed and was accompanied by increased osteoclastic activity and reduced trabecular bone formation. Treatment of wild-type condylar explants with recombinant FGF2/FGF9 decreased Ptch1 and PTHrP expression in superficial/polymorphic layers and proliferation in chondroprogenitors. We also observed early degenerative changes of condylar articular cartilage, abnormal development of the articular eminence/glenoid fossa in the TMJ, and fusion of the articular disc. Analysis of our data indicates that the activating FgfR3(P244R) mutation disturbs TMJ developmental processes, likely by reducing hedgehog signaling and endochondral ossification. We suggest that a balance between FGF and hedgehog signaling pathways is critical for the integrity of TMJ development and for the maintenance of cellular organization.


Assuntos
Craniossinostoses/genética , Fatores de Crescimento de Fibroblastos/fisiologia , Côndilo Mandibular/anormalidades , Receptor Tipo 3 de Fator de Crescimento de Fibroblastos/genética , Articulação Temporomandibular/anormalidades , Animais , Cartilagem Articular/anormalidades , Condrogênese/genética , Craniossinostoses/patologia , Técnicas de Introdução de Genes , Proteínas Hedgehog/fisiologia , Camundongos , Camundongos Mutantes , Mutação , Osteogênese/genética , Transdução de Sinais/genética , Osso Temporal/anormalidades
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