Health indicators and contaminant levels of a critically endangered species in the Gironde estuary, the European sturgeon.

The European sturgeon, Acipenser sturio, is a highly endangered species that almost disappeared in the last decades. Thanks to yearly restocking of the population, this species is still found in the Gironde estuary (France), where juveniles grow during several years before leaving to the ocean. The aims of this study were to evaluate the pressure exerted on these fish by known organic and inorganic contaminants during their stay at the Gironde estuary, and to get information on the fish's health in this context. Monthly captures over the year 2014 provided 87 fish from the cohorts 2012 and 2013 mainly, and from cohorts 2008, 2009, and 2011, all fish born in hatchery. We report the very first analyses of contaminant levels and of biological markers measured in the blood of these fish. Low inorganic contamination was found, composed of seven metals mainly Zn (< 5 µg mL-1), Fe (< 1.5 µg mL-1), Cu (< 0.8 µg mL-1), Se (< 0.8 µg mL-1), As (< 0.25 µg mL-1), Co (< 0.14 µg mL-1), and Mn (< 0.03 µg mL-1). Concerning persistent organic contaminants, the sum of seven PCBs varied from 1 to 10 ng g-1 plasma, that of eight OCPs from 0.1 to 1 ng g-1, and that of eight PBDEs from 10 to 100 pg g-1. Higher levels of contaminants were measured during spring as compared to summer. The sex steroid hormone plasma levels (estradiol, testosterone, and 11-ketotestosterone) were quite low, which was predictable for juveniles. The transcription of reproduction-involved genes (EstR, AR, LHR, sox9) in blood cells was demonstrated for the first time. Some of them were correlated with organic contaminant levels PCBs and OCPs. Other gene transcriptions (sodCu and bax) were correlated with PCBs and OCPs. However, the DNA damage level measured here as comet tail DNA and micronuclei ratio in red blood cells were in the very low range of the values commonly obtained in fish from pristine areas. The data presented here can serve as a reference base for future monitoring of this population of sturgeons.

Investigation of the spatial variability of poly- and perfluoroalkyl substance trophic magnification in selected riverine ecosystems.

The occurrence at different trophic levels of 17 poly- and perfluoroalkyl substances (PFASs), including perfluoroalkyl acids (PFAAs) and some of their precursors (e.g., perfluoroalkane sulfonamides, 6:2 fluorotelomer sulfonate (6:2 FTSA)), was investigated in riverine freshwater food webs in Southeastern France. Two fish species (Barbus barbus and Squalius cephalus) and various invertebrate taxa were collected in five rivers to assess the spatial variability of trophic magnification factors (TMFs). Particular attention was devoted to sample and data processing to minimize potential biases associated with the TMF determination. Fish were significantly more contaminated than invertebrates (ΣPFAS = 7-1811 vs. 0.9-213 ng g-1 wet weight (ww)). Those from the Rhône River presented significantly higher levels due to high concentrations of perfluoroundecanoic acid (406 ng g-1 ww) and perfluorotridecanoic acid (566 ng g-1 ww) ascribed to an industrial point source. Perfluorooctane sulfonate (PFOS) was dominant at the other sites (concentration range = 3.6-134 ng g-1 ww). Two linear regression models were compared (i.e., Kendall regression vs. Generalized Linear Mixed-Effect Model, GLMM). Results showed that TMFs calculated using the non-weighted Kendall regression were higher than those obtained using the GLMM approach. GLMM-based TMFs were consistently >1 for C9-C14 perfluorocarboxylic acids (PFCAs), PFOS and perfluorodecane sulfonate (PFDS), indicating their apparent biomagnification in the investigated food webs. Comparatively, 6:2 FTSA and N-ethylperfluorooctane sulfonamidoacetic acid (N-EtFOSAA) were less often detected and were not significantly biomagnified, probably because of metabolization. TMF estimates were generally consistent across sites although some PFASs (in particular C9, C10 and C13 PFCAs) displayed higher variability, due to a unique extreme value that may have resulted from the contribution of unattributed precursor biotransformation.

Evidence for the Trophic Transfer of Perfluoroalkylated Substances in a Temperate Macrotidal Estuary.

The present survey examines the trophodynamics of a suite of 19 perfluoroalkyl substances (PFASs) in a temperate macrotidal estuary (Gironde, SW France). Across the 147 biota samples (18 taxa) collected, perfluorooctane sulfonate (PFOS), perfluorooctane sulfonamide (FOSA), and C8-C14 perfluoroalkyl carboxylates (PFCAs) were the most-recurrent analytes. ΣPFASs ranged between 0.66-45 ng per g of wet weight of the whole body. Benthic organisms had relatively high ΣPFASs compared to demersal organisms and displayed specific composition profiles with higher relative abundances of C8 and C9 PFCAs. Trophic magnification factors (TMFs) were determined through the use of linear mixed effect models including censored data, thereby considering data below detection limits as well as the interspecific variability of δ15N and PFAS levels (random effects). TMFs were almost consistently >1 in the benthic food web as well as when considering all data pooled together, providing evidence for the biomagnification of several PFASs in estuarine environments. In addition, in contrast with previous observations, TMFs determined in the estuarine benthic web were found to significantly decrease with increasing chain length for C8-C14 PFCAs and C6-C8 perfluoroalkyl sulfonates. This suggests that PFAS chemical structure might not be necessarily predictive of TMFs, which are also influenced by the trophic web characteristics.