RESUMO
The aim of the cooperation between the Laboratory of Leucocyte and Platelet Immunology, Institute of Haematology and Blood Donation Stations was typing of anti-HLA sera from the blood of pregnant women. During 18 months of this cooperation 477 sera were studied. In 255 sera (53.1%) lymphocytotoxic antibodies were demonstrated and their specificity was determined. The types anti-HLA sera will be used for determination of HLA antigens in blood donors donating blood in the Stations. The results of these determinations, technical problems and conclusions drawn from this cooperation are discussed. Continuation of this cooperation will contribute to further limitation of the import of anti-HLA sera.
Assuntos
Soro Antilinfocitário/análise , Antígenos HLA/imunologia , Gravidez/imunologia , Tipagem e Reações Cruzadas Sanguíneas , Feminino , HumanosAssuntos
Anafilaxia/etiologia , Antígenos de Grupos Sanguíneos/imunologia , Incompatibilidade de Grupos Sanguíneos/imunologia , Sistema do Grupo Sanguíneo Duffy/imunologia , Sistema do Grupo Sanguíneo Rh-Hr/imunologia , Reação Transfusional , Adulto , Incompatibilidade de Grupos Sanguíneos/diagnóstico , Reações Cruzadas , Feminino , HumanosRESUMO
A method for eluting IgG antibodies from erythrocytes coated with anti-Rh immune antibodies has been developed, using temperature 56 degrees C as decomplexing factor and ion-exchange on DEAE Sephadex A-50 as antigen-binding factor.
