Canopy-cooling systems applied on avocado trees to mitigate heatwaves damages.

With climate change, spring heatwaves have become frequent in the Mediterranean region. High temperatures combined with wind and low humidity are problematic for subtropical crops adapted to high humidity and mild climate. Avocado is a valuable crop-nutritionally and economically-and many new orchards are planted in Mediterranean areas. Spring heatwaves increase avocado fruitlets dropping, severely decreasing yields. Addressing and solving the problem are necessary to maintain the crop's profitability. This study presents a sprinkler-based canopy cooling method that uses the existing pressurized irrigation system. The study aimed to test the system's performance during spring heatwaves, after the flowering season, in avocado orchards cultivated in a semi-arid region. The experiments examined the effect of various sprinkler types with varying flow rates and installation methods: sprayers, sprinklers and pulsing sprinklers, on foliage temperature, stem water potential, salt accumulation in the leaf, fruitlet survival and yield. The system reduced leaf temperatures by approximately 10 °C, significantly decreasing the trees' drought stress and increasing yields by 8-12%. Using low-quality water is possible, but requires adjustments to avoid salt damage to the leaves. The system can mitigate heat stress, and provides a relatively simple solution for handling spring heatwaves. The evaporative cooling system is modeled for semi-desert and desert conditions; the dry, windy climate contributes to the method's effectiveness.

The metabolic reserves, carbohydrate balance and nutritional status of jojoba (Simmondsia chinensis), in relation to its annual cycle and fruit load.

Jojoba (Simmondsia chinensis (Link) Schneider) holds high industrial value and an extended cultivation trend. Despite its increased importance, there is a lack of fundamental information about its metabolic reserves and development. Our objective was to characterise metabolite allocation and fluctuations in the carbohydrate and nutrient balance of jojoba plants, as affected by fruit load and the plant's annual cycle. Metabolite profiles were performed for each organ. Soluble carbohydrates (SC) and starch concentrations were surveyed in underground and aboveground organs of high-yield and fruit-removed plants. Simultaneously, nitrogen, potassium and phosphorus were determined in the leaves to evaluate the plant's nutritional status. We found that sucrose and pinitol were the most abundant sugars in all jojoba organs. Each sugar had a 'preferred' organ: glucose was accumulated mainly in the leaves, sucrose and pinitol in woody branches, and fructose in the trunk wood. We found that fruit load significantly influenced the carbohydrate levels in green branches, trunk wood and thin roots. The phenological stage strongly affected the SC-starch balance. Among the examined minerals, only the leaf potassium level was significantly influenced by fruit load. We conclude that jojoba's nutrient and carbohydrate balance is affected by fruit load and the phenological stage, and describe the organ-specific metabolic reserves.

It takes two: Reciprocal scion-rootstock relationships enable salt tolerance in 'Hass' avocado.

Commercial avocado orchards typically consist of composite trees. Avocado is salt-sensitive, suffering from substantial growth and production depreciation when exposed to high sodium and chloride levels. Salt ions penetrate the roots and are subsequently transferred to the foliage. Hence, understanding distinct physiological responses of grafted avocado plant organs to salinity is of great interest. We compared the ion, metabolite and lipid profiles of leaves, roots and trunk drillings of mature 'Hass' scion grafted onto two different rootstocks during gradual exposure to salinity. We found that one rootstock, VC840, did not restrict the transport of irrigation solution components to the scion, leading to salt accumulation in the trunk and leaves. The other rootstock, VC152, functioned selectively, moderating the movement of toxic ions to the scion organs by accumulating them in the roots. The leaves of the scion grafted on the selective rootstock acquired the standard level of essential minerals without being exposed to excessive salt concentrations. However, this came with an energetic cost as the leaves transferred carbohydrates and storage lipids downward to the rootstock organs, which became a strong sink. We conclude that mutual scion-rootstock relationships enable marked tolerance to salt stress through selective ion transport and metabolic modifications.

Rootstock-Dependent Response of Hass Avocado to Salt Stress.

Salt stress is a major limiting factor in avocado (Persea americana) cultivation, exacerbated by global trends towards scarcity of high-quality water for irrigation. Israeli avocado orchards have been irrigated with relatively high-salinity recycled municipal wastewater for over three decades, over which time rootstocks were selected for salt-tolerance. This study's objective was to evaluate the physiological salt response of avocado as a function of the rootstock. We irrigated fruit-bearing 'Hass' trees grafted on 20 different local and introduced rootstocks with water high in salts (electrical conductivity of 1.4-1.5 dS/m). The selected rootstocks represent a wide range of genetic backgrounds, propagation methods, and horticultural characteristics. We investigated tree physiology and development during two years of salt exposure by measuring Cl and Na leaf concentrations, leaf osmolality, visible damages, trunk circumference, LAI, CO2 assimilation, stomatal conductance, spectral reflectance, stem water potential, trichomes density, and yield. We found a significant effect of the rootstocks on stress indicators, vegetative and reproductive development, leaf morphogenesis and photosynthesis rates. The most salt-sensitive rootstocks were VC 840, Dusa, and VC 802, while the least sensitive were VC 159, VC 140, and VC 152. We conclude that the rootstock strongly influences avocado tree response to salinity exposure in terms of physiology, anatomy, and development.

When vegetation indicates reproduction: The affinity between leaf morphology and flowering commitment in the lily meristem.

At the reproductive stage, lily plants bear two morphological types of mature leaves, one at the lower and one at the upper part of the stem. At the vegetative stage, all the leaves are similar to each other and to the reproductive plant's lower leaves. This heterophylly has not yet been explored. In this study, we show that it is not a result of the plant's age but rather an outcome of floral induction. The induction appears as an on-going process, during which the meristem still produces leaves but progressively becomes committed to reproduction. This intermediate period lasts until the ultimate switch to flower primordia occurs. The leaves produced during floral induction, termed here as "inductive," appear at the upper part of the stem. Besides their typical higher stomata density, these leaves have a poly-layered palisade mesophyll, whose cells exhibit a unique morphology and contain more chlorophyll than leaves of vegetative plants. These leaves display higher carbon assimilation, soluble sugar production, and chloroplast-lipid accumulation. Accordingly, genes associated with stomata, chloroplast, and photosynthesis are upregulated in these leaves. Our results were obtained when floral induction was achieved either by vernalization or photoperiod signals, ruling out a mere environmental effect. We suggest that lily plants prepare themselves for the high-energy-demanding bloom by producing leaves with enhanced photosynthetic capacity, leading to an increase in soluble sugars. These novel findings introduce an adjacent affinity between photosynthesis and flowering and provide a nondestructive tool for identifying the plant's developmental stage-vegetative or reproductive.

The Effect of Macronutrient Availability on Pomegranate Reproductive Development.

Pomegranate cultivation has expanded significantly in the last two decades. However, there is limited information on its fertilization requirements and the effect of macronutrient availability on its reproductive development. Two commercial pomegranate cultivars-"Wonderful" and "Emek"-were grown in 500-L containers for 3 years, using a fertigation system. Development and reproduction indices were measured to explore the trees' responses to elevated levels of nitrogen (N), phosphorus (P) and potassium (K) in the irrigation solution. Andromonoecy rate was affected by nutrient levels only in the first year of the experiment, with higher levels of N and P leading to a greater proportion of hermaphrodites out of total flowers. P level had a positive effect on the total number of hermaphrodites per tree in both varieties. Differences recorded between hermaphroditic and staminate flowers included nutrient concentrations and dry weight. Fruit set and aril number were positively affected by N concentration in the irrigation solution. We conclude that only a severe deficiency of N and P affects the andromonoecy trait, and that at the levels examined in this study, K hardly influences pomegranate reproduction.

Management of Verticillium Wilt of Avocado Using Tolerant Rootstocks.

The global avocado industry is growing, and farmers are seeking to expand their plantations. However, many lands suitable for avocado planting were previously cultivated with hosts of the soil-borne fungal pathogen Verticillium dahliae, which is the causal agent of Verticillium wilt (VW). VW can seriously impair avocado orchards, and therefore, planting on infested soil is not recommended. The use of different rootstock types allows avocado cultivation in various regions with diverse biotic and abiotic constraints. Hence, we tested whether genetic variance among rootstocks may also be used to manage avocado VW. Six hundred trees, mostly Hass and some Ettinger, grafted on 23 selected rootstocks were evaluated for five years in a highly V. dahliae-inoculated plot for VW symptoms, fungal infection, and productivity. The selected rootstocks displayed a significant variation related to VW tolerance, and productive avocado rootstocks with potential VW tolerance were identified. Moreover, the rootstock productivity appears to correlate negatively to the susceptibility level. In conclusion, planting susceptible rootstocks (e.g., VC66, VC152, and VC26) in infested soil increases the likelihood of massive tree loss and low productivity. Whereas, tolerant rootstocks (e.g., VC804 and Dusa) may restrict VW and enable avocado cultivation on infested soils.

The proof is in the bulb: glycerol influences key stages of lily development.

A bulb is a whole plant condensed into an underground organ. A geophyte's bulb comprises both food reserves and important developmental history that may affect its whole growth. In Easter lily (Lilium longiflorum), bulb size is associated with the plant's flowering pathway - vernalization or photoperiod - and also affects sprouting, flower quality and abortion rate. The aim of this study was to investigate the reasons for the major physiological differences between large and small bulbs. Lily bulbs start their development from secondary meristems along the stem, with large bulbs being heavier and bear more scales than small ones. Peeling the outer scales of a large bulb converts its physiological responses into those of a small bulb, implying that the physiological discrepancies in plants developing from large or small bulbs are mediated by factors inherent to the bulb. We therefore performed broad analyses of the metabolite composition in the scales of bulbs subjected to temperature regimes affecting further plant development. We found a striking association between the level of glycerol, a primary metabolite mostly synthesized in the outer scales, and a delay in sprouting and flowering time, and reduction in abortion rate. Exogenous glycerol application to the bulbs before planting corroborated these results. Moreover, transcriptome analyses showed that flowering-promoting gene expression was downregulated in the bulb after glycerol treatment, while potential flowering inhibitor as well as a dormancy-related gene expressions were upregulated. Based on these studies, we postulate that glycerol is a major factor influencing both vegetative and reproductive development in lily.

The metabolic (under)groundwork of the lily bulb toward sprouting.

Large bulbs of Lilium longiflorum have an obligatory cold requirement to flower. Bulb cooling is widely used to induce and accelerate flowering. However, in-depth investigations of the effect of bulb cooling on major landmarks of plant development are lacking. It has been demonstrated that low temperature induces carbohydrate degradation, yet integrative studies on metabolic changes occurring in the bulb are not available. We detected that cold exposure mainly hastened bulb sprouting, rather than floral transition or blooming. Metabolite profiling of cooled and non-cooled bulbs was carried out, revealing cold-induced accumulation of soluble sugars, lipids and specific amino acids, and a significant reduction in tricarboxylic acid (TCA)-cycle elements. We observed that metabolic pathways located in the cytosol - including glycolysis, lipid synthesis and part of the gamma-Aminobutyric acid (GABA) shunt - were enhanced by cold exposure, while mitochondrial metabolism - namely the TCA cycle - was reduced by cold. We suggest a physiological model accounting for this metabolic discrepancy.