Use of an ion-selective membrane electrode for the determination of the active components in intestopan.

The conditions for the determination of broxyquinoline and brobenzoxaldine, the active components of "Intestopan", by use of ion-selective membrane electrodes are described. Broxyquinoline is determined directly through precipitation with CuSO(4), and brobenzoxaldine is first hydrolysed in alkaline solution and the product precipitated with CuSO(4). In both cases the CuSO(4) in excess is determined by potentiometric titration at pH 5.6 with EDTA, a Cu(2+)-selective electrode being used for end-point detection.

The effect of new adamantane derivatives on experimental influenza infection in mice.

Two newly synthesized derivatives of 1-aminoadamantane hydrochloride--CF.01.01 and CF.01.06--were assayed for their anti-influenza efficacy. Aqueous solutions of the drugs (200 gamma/0.1 ml) were administered intranasally to mice at 24 hours and 5 hours or at 24 hours, 5 hours and 1 hour prior to intranasal inoculation of influenza virus A/PR/8/34 (H1N1). Compound CF.01.06 caused a significant decrease of the HA titer in the lungs of virus-infected mice. There was no change in mortality rate and the increase in mean survival time was at the limit of statistical significance.