RESUMO
BACKGROUND: Excessive accumulation of adipose tissue in cultured fish is an outstanding problem in aquaculture. To understand the development of adiposity, it is crucial to identify the genes which expression is associated with adipogenic differentiation. Therefore, the transcriptomic profile at different time points (days 3, 8, 15 and 21) along primary culture development of rainbow trout preadipocytes has been investigated using an Agilent trout oligo microarray. RESULTS: Our analysis identified 4026 genes differentially expressed (fold-change >3) that were divided into two major clusters corresponding to the main phases observed during the preadipocyte culture: proliferation and differentiation. Proliferation cluster comprised 1028 genes up-regulated from days 3 to 8 of culture meanwhile the differentiation cluster was characterized by 2140 induced genes from days 15 to 21. Proliferation was characterized by enrichment in genes involved in basic cellular and metabolic processes (transcription, ribosome biogenesis, translation and protein folding), cellular remodelling and autophagy. In addition, the implication of the eicosanoid signalling pathway was highlighted during this phase. On the other hand, the terminal differentiation phase was enriched with genes involved in energy production, lipid and carbohydrate metabolism. Moreover, during this phase an enrichment in genes involved in the formation of the lipid droplets was evidenced as well as the activation of the thyroid-receptor/retinoic X receptor (TR/RXR) and the peroxisome proliferator activated receptors (PPARs) signalling pathways. The whole adipogenic process was driven by a coordinated activation of transcription factors and epigenetic modulators. CONCLUSIONS: Overall, our study demonstrates the coordinated expression of functionally related genes during proliferation and differentiation of rainbow trout adipocyte cells. Furthermore, the information generated will allow future investigations of specific genes involved in particular stages of fish adipogenesis.
Assuntos
Adipócitos/fisiologia , Adipogenia , Transcriptoma , Animais , Proliferação de Células , Células Cultivadas , Proteínas de Peixes/genética , Proteínas de Peixes/metabolismo , Oncorhynchus mykiss/genética , Oncorhynchus mykiss/metabolismo , Transdução de Sinais , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismoRESUMO
The dramatic increase in myotomal muscle mass in teleosts appears to be related to their sustained ability to produce new fibres in the growing myotomal muscle. To describe muscle fibre input dynamics in trout (Oncorhynchus mykiss), we generated a stable transgenic line carrying green fluorescent protein (GFP) cDNA driven by the myogenin promoter. In this myog:GFP transgenic line, muscle cell recruitment is revealed by the appearance of fluorescent, small, nascent muscle fibres. The myog:GFP transgenic line displayed fibre formation patterns in the developing trout and showed that the production of new fluorescent myofibres (muscle hyperplasia) is prevalent in the juvenile stage but progressively decreases to eventually cease at approximately 18â months post-fertilisation. However, fluorescent, nascent myofibres were formed de novo in injured muscle of aged trout, indicating that the inhibition of myofibre formation associated with trout ageing cannot be attributed to the lack of recruitable myogenic cells but rather to changes in the myogenic cell microenvironment. Additionally, the myog:GFP transgenic line demonstrated that myofibre production persists during starvation.
Assuntos
Proteínas de Fluorescência Verde/metabolismo , Fibras Musculares Esqueléticas/fisiologia , Miogenina/metabolismo , Oncorhynchus mykiss/embriologia , Oncorhynchus mykiss/crescimento & desenvolvimento , Envelhecimento , Animais , Animais Geneticamente Modificados , Proliferação de Células/fisiologia , Proteínas de Fluorescência Verde/genética , Desenvolvimento Muscular , Miogenina/genética , Oncorhynchus mykiss/genética , Regiões Promotoras GenéticasRESUMO
The trunk muscle in fish is organized as longitudinal series of myomeres which are separated by sheets of connective tissue called myoseptum to which myofibers attach. In this study we show in the trout that the myoseptum separating two somites is initially acellular and composed of matricial components such as fibronectin, laminin and collagen I. However, myoseptal cells forming a continuum with skeletogenic cells surrounding axial structures are observed between adjacent myotomes after the completion of somitogenesis. The myoseptal cells do not express myogenic markers such as Pax3, Pax7 and myogenin but express several tendon-associated collagens including col1a1, col5a2 and col12a1 and angiopoietin-like 7, which is a secreted molecule involved in matrix remodelling. Using col1a1 as a marker gene, we observed in developing trout embryo an initial labelling in disseminating cells ventral to the myotome. Later, labelled cells were found more dorsally encircling the notochord or invading the intermyotomal space. This opens the possibility that the sclerotome gives rise not only to skeletogenic mesenchymal cells, as previously reported, but also to myoseptal cells. We furthermore show that myoseptal cells differ from skeletogenic cells found around the notochord by the specific expression of Scleraxis, a distinctive marker of tendon cells in amniotes. In conclusion, the location, the molecular signature and the possible sclerotomal origin of the myoseptal cells suggest that the fish myoseptal cells are homologous to the axial tenocytes in amniotes.
Assuntos
Tecido Conjuntivo/embriologia , Tendões/citologia , Tendões/embriologia , Truta/embriologia , Animais , Fatores de Transcrição Hélice-Alça-Hélice Básicos/metabolismo , Colágeno Tipo I/metabolismo , Matriz Extracelular/metabolismo , Regulação da Expressão Gênica no Desenvolvimento , Mesoderma/citologia , Movimento , Somitos/citologia , Truta/metabolismoRESUMO
BACKGROUND: A unique feature of fish is that new muscle fibres continue to be produced throughout much of the life cycle; a process termed muscle hyperplasia. In trout, this process begins in the late embryo stage and occurs in both a discrete, continuous layer at the surface of the primary myotome (stratified hyperplasia) and between existing muscle fibres throughout the myotome (mosaic hyperplasia). In post-larval stages, muscle hyperplasia is only of the mosaic type and persists until 40% of the maximum body length is reached. To characterise the genetic basis of myotube neoformation in trout, we combined laser capture microdissection and microarray analysis to compare the transcriptome of hyperplastic regions of the late embryo myotome with that of adult myotomal muscle, which displays only limited hyperplasia. RESULTS: Gene expression was analysed using Agilent trout oligo microarrays. Our analysis identified more than 6800 transcripts that were significantly up-regulated in the superficial hyperplastic zones of the late embryonic myotome compared to adult myotomal muscle. In addition to Pax3, Pax7 and the fundamental myogenic basic helix-loop-helix regulators, we identified a large set of up-regulated transcriptional factors, including Myc paralogs, members of Hes family and many homeobox-containing transcriptional regulators. Other cell-autonomous regulators overexpressed in hyperplastic zones included a large set of cell surface proteins belonging to the Ig superfamily. Among the secreted molecules found to be overexpressed in hyperplastic areas, we noted growth factors as well as signalling molecules. A novel finding in our study is that many genes that regulate planar cell polarity (PCP) were overexpressed in superficial hyperplastic zones, suggesting that the PCP pathway is involved in the oriented elongation of the neofibres. CONCLUSION: The results obtained in this study provide a valuable resource for further analysis of novel genes potentially involved in hyperplastic muscle growth in fish. Ultimately, this study could yield insights into particular genes, pathways or cellular processes that may stimulate muscle regeneration in other vertebrates.
Assuntos
Desenvolvimento Embrionário/genética , Hiperplasia/genética , Análise de Sequência com Séries de Oligonucleotídeos/métodos , Truta , Animais , Perfilação da Expressão Gênica , Regulação da Expressão Gênica no Desenvolvimento , Humanos , Hiperplasia/patologia , Microdissecção e Captura a Laser , Músculo Esquelético/citologia , Músculo Esquelético/crescimento & desenvolvimento , Músculo Esquelético/metabolismo , Truta/genética , Truta/crescimento & desenvolvimentoRESUMO
The objective of this study was to investigate the expression of two promyogenic cell surface adhesion receptors, N- and M-cadherin, in developing trout (Oncorhynchus mykiss) somite, taking account of the recent identification of a dermomyotome-like epithelium in teleosts. In situ hybridization showed that N-cadherin was expressed throughout the paraxial mesoderm and nascent somite. As the somite matured, N-cadherin expression disappeared ventrally from the sclerotome, and then mediolaterally from the differentiating slow and fast muscle cells of the embryonic myotome, to become finally restricted to the undifferentiated myogenic precursors forming the dermomyotome-like epithelium that surrounds the embryonic myotome. By contrast, M-cadherin, which was transcribed in the differentiating embryonic myotome, was never expressed in the dermomyotome-like epithelium. In late-stage trout embryos, M-cadherin transcript was only detected at the periphery of the expanding myotome, where muscle cells stemming from the N-cadherin positive dermomyotome-like epithelium differentiate. Collectively, our results support the view that, in trout embryo, N-cadherin is associated with muscle cell immaturity while M-cadherin is associated with muscle cell maturation and differentiation and this during the two successive phases of myogenesis.
Assuntos
Caderinas/genética , Regulação da Expressão Gênica no Desenvolvimento , Desenvolvimento Muscular/fisiologia , Mioblastos/metabolismo , Oncorhynchus mykiss/embriologia , Animais , Diferenciação Celular , Epitélio/metabolismo , Perfilação da Expressão Gênica , Mioblastos/citologia , Somitos/metabolismoRESUMO
While in mammals, the IGF/IGFBP system is known to be involved in adipose tissue growth, the presence of such a system in fish is as yet undetermined. The present work aimed at investigating the influence of regional localization of adipose tissues and cell types on the expression of this system. Using quantitative real-time PCR (qPCR), the presence of IGFs, IGFBPs, IGFBP-rPs, and IGFR I (insulin-like growth factors, IGF-binding proteins, IGFBP-related proteins, type I IGF receptor) was studied in crude subcutaneous and visceral fat depots as well as in isolated stromal vascular (SV) cells and mature adipocytes from the latter depot in the prepubescent female rainbow trout. In adipose tissues, no differences were observed in the transcript expressions of IGFs and IGFBPs (1-6) and rP1 relative to their regional localization. On the other hand, the two paralogues of IGFR I were more strongly expressed in visceral than in subcutaneous depots which could be related to a differential receptivity to IGF between fat depots. The amount of IGF-I and IGFR Ia transcripts is larger in mature adipocytes than in SV cells, while a similar level of expression was observed for IGF-II and IGFR Ib. IGFBP-1, -2a, -4, -5, -6, and -rP1 were more strongly expressed in the SV cells than in adipocytes, while IGFBP-2b displayed comparable expression. These results indicate that the IGF/IGFBP system is expressed in rainbow trout fat depots, whatever their regional origin, and that cell types, i.e., SV cells or mature adipocytes, influence its expression.
Assuntos
Proteínas de Ligação a Fator de Crescimento Semelhante a Insulina/metabolismo , Gordura Intra-Abdominal/metabolismo , Oncorhynchus mykiss/metabolismo , Somatomedinas/metabolismo , Gordura Subcutânea/metabolismo , Adipogenia , Animais , Feminino , Proteínas de Ligação a Fator de Crescimento Semelhante a Insulina/genética , Oncorhynchus mykiss/genética , Reação em Cadeia da Polimerase , Receptor IGF Tipo 1/genética , Receptor IGF Tipo 1/metabolismo , Somatomedinas/genética , Células Estromais/metabolismoRESUMO
The contribution of growth history and flagella to adhesion of Listeria monocytogenes was analysed. An in-frame deletion on the flagellin encoding gene (flaA) was performed in L. monocytogenes EGD-e to compare its adhesion ability with the parental strain, after cultivation at various pH values and temperatures. The pH, as well as the temperature, affected the adhesion of L. monocytogenes EGD-e. In addition, the adhesion of L. monocytogenes EGD-e was reduced in energy-depressed cells. Conversely, the physicochemical bacterial surface characteristics affected by growth history did not influence the adhesion. Adhesion variations observed among environmental and clinical strains was attributed to the flagella. The naturally aflagellated strains resulted in an adhesion capacity similar to that observed for mutants and parental strains cultivated under flagellum expression repressing conditions. However, L. monocytogenes is able to adhere to inert surfaces through a residual adhesion process without flagella. All these observations emphasize the importance to consider the food environmental factors in the risk assessment of L. monocytogenes in food industry.
Assuntos
Aderência Bacteriana , Flagelos/fisiologia , Listeria monocytogenes/fisiologia , Poliestirenos , Meios de Cultura/metabolismo , Flagelos/genética , Flagelina/genética , Flagelina/metabolismo , Humanos , Concentração de Íons de Hidrogênio , Listeria monocytogenes/genética , Listeria monocytogenes/crescimento & desenvolvimento , Listeria monocytogenes/isolamento & purificação , Listeriose/microbiologia , TemperaturaRESUMO
The effect was studied of supplementing the recovery medium with lysozyme, glucose, NaCl, and MgSO4 on the apparent heat resistance of four strains of Bacillus cereus . The composition of an optimal medium (allowing the germination and growth of all surviving spores) and of a selective medium (inhibiting injured spores) was then determined: the optimal medium consisted of nutrient agar supplemented with 50 × 10-6 g of lysozyme and 0.5 g of MgSO4 per liter. The selective medium had the same composition but was supplemented with 15 g of NaCl per liter. The injury and lethality were analyzed of heat treatment of spores of four Bacillus cereus strains in phosphate buffer and in mechanically separated poultry meat. The four strains tested exhibited great differences in injury and death rates, and considerable differences could be observed in survivor counts when spores were suspended in buffer or in meat. Moreover, variability in behavior when the strains were heat treated in buffer could not be extrapolated to the same strains suspended in food. These data showed that it is necessary to be very careful when evaluating potential hazards from B. cereus spores in various foods; heating medium and strain selection must be considered to evaluate the efficiency of heat treatments.