Urbanization and Carpobrotus edulis invasion alter the diversity and composition of soil bacterial communities in coastal areas.

Coastal dunes are ecosystems of high conservation value that are strongly impacted by human disturbances and biological invasions in many parts of the world. Here, we assessed how urbanization and Carpobrotus edulis invasion affect soil bacterial communities on the north-western coast of Spain, by comparing the diversity, structure and composition of soil bacterial communities in invaded and uninvaded soils from urban and natural coastal dune areas. Our results suggest that coastal dune bacterial communities contain large numbers of rare taxa, mainly belonging to the phyla Actinobacteria and Proteobacteria. We found that the presence of the invasive C. edulis increased the diversity of soil bacteria and changed community composition, while urbanization only influenced bacterial community composition. Furthermore, the effects of invasion on community composition were conditional on urbanization. These results were contrary to predictions, as both C. edulis invasion and urbanization have been shown to affect soil abiotic conditions of the studied coastal dunes in a similar manner, and therefore were expected to have similar effects on soil bacterial communities. Our results suggest that other factors (e.g. pollution) might be influencing the impact of urbanization on soil bacterial communities, preventing an increase in the diversity of soil bacteria in urban areas.

Drifting away. Seawater survival and stochastic transport of the invasive Carpobrotus edulis.

Coastal areas are vulnerable and fluctuating habitats that include highly valuable spaces for habitat and species conservation and, at the same time, they are among the most invaded ecosystems worldwide. Occupying large areas within Mediterranean-climate coastlines, the "ecosystem engineer" Carpobrotus edulis appears as a menace for coastal biodiversity and ecosystem services. By combining the observation, current distribution, glasshouse experiment, and dispersion modeling, we aim to achieve a better understanding of the successful invasion process and potential dispersion patterns of C. edulis. We analyzed the response of plant propagules (seeds and plant fragments) to seawater immersion during increasing periods of time (up to 144 h). After 2 months of growth, plant fragments showed a total survival rate (100%) indicating high tolerance to salinity. During this time, fragment length was increased (up to 60%) and root length was higher than control in all cases. Also, immersed fragments consistently accumulated more biomass than control fragments. After two months of growth, photosynthetic parameters (Fv'/Fm', ΦNO, and ΦII) remained stable compared to control fragments. Physiologically, osmolyte and pigment content did not evidence significant changes regardless of immersion time. Based on the capacity of propagules to survive seawater immersion, we modeled the potential transport of C. edulis by combining an oceanic model (ROMS-AGRIF) with a particle-tracking model. Results indicated that propagules may travel variable distances maintaining physiological viability. Our model suggested that short-scale circulation would be the dominant process, however, long-scale circulation of propagules may be successfully accomplished in <6 days. Furthermore, under optimal conditions (southerly winds dominance), propagules may even travel large distances (250 km alongshore). Modeling transport processes, in combination with the dynamics of introduction and expansion, will contribute to a better understanding of the invasive mechanisms of C. edulis and, consequently, to design preventive strategies to reduce the impact of plant invasion.

Clonal integration facilitates the colonization of drought environments by plant invaders.

Biological invasion represents one of the main threats for biodiversity conservation at the global scale. Identifying the mechanisms underlying the process of biological invasions is a crucial objective in the prediction of scenarios of future invasions and the mitigation of their impacts. In this sense, some plant attributes might better explain the success of invasive plant species than others. Recently, clonal growth has been identified as an attribute that could contribute to the invasiveness of plants. In this experiment, we aim to determine the effect of physiological integration (one of the most striking attributes associated with clonal growth) in the performance (at morphological and physiological levels) of the aggressive invader Carpobrotus edulis, when occupying stressful environments. To achieve this objective we performed a greenhouse experiment in which apical ramets of C. edulis were water-stressed and the connection with the basal ramets was either left intact (physiological integration is allowed) or severed (physiological integration is impeded). Our results show that clonal integration allowed apical ramets to buffer drought stress in terms of photochemical activity, and as a consequence, to increase their growth in comparison with severed apical ramets. Interestingly, this increase in biomass was mainly due to the production of aboveground structures, increasing the spread along the soil surface, and consequently having important implications for the colonization success of new environments by this aggressive invader.