Validation and efficacy of transgenerational mass marking of otoliths in viviparous fish larvae.

Transgenerational mass marking of viviparous fish larvae in vivo was validated by intra-muscular injection of elemental strontium chloride (SrCl(2)) in gestating females and detection of the Sr in the otoliths of developing larvae. All otoliths of brown rockfish Sebastes auriculatus larvae produced from SrCl(2)-injected females showed enriched Sr:Ca ratios near the otolith edges, and the signatures did not appear to be affected by the anterior, centre and posterior positions of larvae within the ovary. Results from the present study indicate that transgenerational marking is a highly reliable technique for marking large numbers of extremely small viviparous fish larvae.

Colon carcinoma discovered in an adolescent during inguinal hernia repair.

Colon carcinoma is an unusual tumor during childhood. The authors report a case of metastatic colonic adenocarcinoma diagnosed in a 14-year-old boy during inguinal hernia repair. To the authors' knowledge, this is the first reported case of this rare disease presenting as an inguinal hernia.