1.
Methods Mol Biol
; 400: 333-9, 2007.
Artigo
em Inglês
| MEDLINE
| ID: mdl-17951744
RESUMO
The authors developed a technique to apply high hydrostatic pressure to giant unilamellar vesicles and to directly observe the consequent structural changes with two-photon fluorescence microscopy imaging using high numerical aperture oil-immersion objectives. The data demonstrate that high pressure has a dramatic effect on the shape of the vesicles, and both fluidity and homogeneity of the membrane.