Evidence-Based Strategies for Warm Compress Therapy in Meibomian Gland Dysfunction.

AIM: Despite promising results from technological therapies like intense pulsed light application, warm compress therapy is a mainstay in meibomian gland dysfunction (MGD). However, applying warm compresses (WC) to the eyelids is palliative rather than curative and not always dispensed with specific instructions. The range of eyelid warming treatments available and lack of clear directives for use creates uncertainty for patients accustomed to explicit dosage information. This report examines data from clinical studies across the past 20 years to identify effective protocols for three types of WC-hot towel, microwavable eye mask, and self-heating eye mask (EM). METHOD: Literature search for studies on WC and MGD published between 2004 and 2023 in English was conducted. Studies wherein hot towel, microwavable EM, and self-heating EM were used in a treatment arm were included and those wherein they served only as control or were used in conjunction with another intervention were excluded. 20 resulting studies were separated into 3 groups: 5 on temperature profiles of WC, 6 with single application of WC, and 9 with repeated applications. Study methods and outcomes were tabulated, and a qualitative review was performed, attending to WC protocol and efficacy, as indicated by measures of tear film, meibomian gland health, and dry eye questionnaires. RESULTS: Data from the aforementioned studies revealed that each method can achieve target eyelid temperature of 40 °C. A single application of WC-ranging from 5 to 20 min-can significantly improve tear quality, while repeated applications significantly relieve symptoms associated with dry eyes from MGD and, in most studies, significantly improve meibomian gland health. Hot towels, however, require frequent reheating to maintain eyelid temperatures above 40 °C, rendering them relatively ineffective in longitudinal studies. Microwavable EM retain heat well across 10 min and were found to improve tear break-up time and/or meibomian gland score. Self-heating EM have variable activation times and were typically applied for longer periods, showing benefits akin to microwavable EM in short-term studies. Studies monitoring compliance indicate greater deviation from protocol with higher application frequencies or longer-term use. Evidence suggests superior heat retention and therapeutic effects on specific contributing factors in MGD (such as Demodex) with moist-heat compress. CONCLUSION: Considering decreased patience adherence to therapy with increased usage frequencies, and balancing needs to provide succinct instructions for various compress types, an advisable strategy is for patients to apply a moist-heat generating EM (microwavable or self-heating) to each eye for at least 10 min, prepared according to manufacturer's instructions.

Are self-test kits still relevant post COVID-19 pandemic? Qualitative study on working adults' perceptions.

BACKGROUND: Other than self-isolation measures, self-testing is likely to reduce the transmission of COVID-19 and may become a valuable approach in future outbreaks of infectious diseases. This study delves into the perceptions and experiences of working adults who utilised COVID-19 self-test kits in Singapore during the post-pandemic period. METHODS: Employing a qualitative descriptive design, in-depth interviews were conducted with 40 working adults from diverse occupations, with a median age of 35 years. Thematic analysis of transcribed interviews was carried out by two independent qualitative research-trained researchers. RESULTS: Facilitators included personal motivations like safeguarding vulnerable individuals and the convenience of user-friendly self-test kits. Proximal environmental factors encompassed situations involving close contacts and large gatherings, while distal factors involved workplace regulations, public health campaigns, mass media influence, and trust in authorised kits. Nonetheless, barriers include discomfort, cost, uncertainty about proper technique, and evolving testing requirements. Positive test results prompted participants to notify contacts and self-isolate, whereas negative results encouraged a return to normalcy. Participants recommend sustained testing for new variants and vulnerable groups, though some view it as unnecessary due to pandemic normalisation. CONCLUSIONS: Self-testing emerged as a social phenomenon influenced by societal obligations and relationships across multiple levels. Effective communication strategies may play a role in fostering trust and ensuring that working adults sustain the practice of self-testing; therefore, further research is needed to explore their potential impact. Such efforts could be valuable for maintaining vigilance and achieving effective disease control in the post-COVID-19 pandemic landscape.

Discovery of Potent Non-Nucleoside Inhibitors of Dengue Viral RNA-Dependent RNA Polymerase from a Fragment Hit Using Structure-Based Drug Design.

The discovery and optimization of non-nucleoside dengue viral RNA-dependent-RNA polymerase (RdRp) inhibitors are described. An X-ray-based fragment screen of Novartis' fragment collection resulted in the identification of a biphenyl acetic acid fragment 3, which bound in the palm subdomain of RdRp. Subsequent optimization of the fragment hit 3, relying on structure-based design, resulted in a >1000-fold improvement in potency in vitro and acquired antidengue activity against all four serotypes with low micromolar EC50 in cell-based assays. The lead candidate 27 interacts with a novel binding pocket in the palm subdomain of the RdRp and exerts a promising activity against all clinically relevant dengue serotypes.

Small molecule inhibitors that selectively block dengue virus methyltransferase.

Crystal structure analysis of Flavivirus methyltransferases uncovered a flavivirus-conserved cavity located next to the binding site for its cofactor, S-adenosyl-methionine (SAM). Chemical derivatization of S-adenosyl-homocysteine (SAH), the product inhibitor of the methylation reaction, with substituents that extend into the identified cavity, generated inhibitors that showed improved and selective activity against dengue virus methyltransferase (MTase), but not related human enzymes. Crystal structure of dengue virus MTase with a bound SAH derivative revealed that its N6-substituent bound in this cavity and induced conformation changes in residues lining the pocket. These findings demonstrate that one of the major hurdles for the development of methyltransferase-based therapeutics, namely selectivity for disease-related methyltransferases, can be overcome.

A survey of lifestyle risk factors for cardiovascular health among young Hong Kong Chinese women: implications for long-term cardiovascular health.

PURPOSE: To establish baseline data in a population of young Chinese women from which lifestyle risk factors for cardiovascular disease (CVD) and the implications for long-term cardiovascular health can be examined. METHODS: In a cross-sectional postal survey, a 125-item questionnaire (a translated version of the Minnesota Women's Health Survey) assessing lifestyle risk factors and perceptions of health was mailed to 987 Hong Kong Chinese nurses aged 26 years and returned by 617, yielding a response rate of 63%. RESULTS: Of the women surveyed, 7% reported they smoked, 21% consumed alcohol regularly, and 32% exercised regularly. Ten percent reported being overweight or obese, 31% were underweight, and 8% used oral contraception. The findings have important implications for the long-term cardiovascular health of this group of young women health professionals. CONCLUSIONS: Baseline data of lifestyle cardiovascular risk factors demonstrate that approximately two thirds of the women in this study are at risk of developing CVD when the findings are interpreted within the classification of CVD risk in women developed by the American Heart Association. Further research is necessary to assess women's awareness of risk factors and perceived personal risk.

Peptide deformylase inhibitors of Mycobacterium tuberculosis: synthesis, structural investigations, and biological results.

Bacterial peptide deformylase (PDF) belongs to a subfamily of metalloproteases catalyzing the removal of the N-terminal formyl group from newly synthesized proteins. We report the synthesis and biological activity of highly potent inhibitors of Mycobacterium tuberculosis (Mtb) PDF enzyme as well as the first X-ray crystal structure of Mtb PDF. Structure-activity relationship and crystallographic data clarified the structural requirements for high enzyme potency and cell based potency. Activities against single and multi-drug-resistant Mtb strains are also reported.

Vocational rehabilitation program for persons with occupational deafness.

INTRODUCTION: A pilot return-to-work program based on the concept of work readiness and three-month job placement with support was developed to enable people with occupational deafness to return to the workforce. METHODS: A convenient sample of six male persons with occupational deafness completed the program. A Pretest-Posttest Single Group Design was utilized to measure the program outcome. Two instruments: the Chinese version of SF-36 Health Assessment Questionnaire and the Chinese version of Lam's Assessment of Stage of employment readiness were used to measure the participants' health status and their work readiness respectively. RESULTS: The preliminary findings indicated that the six participants showed improvement in their general health and work readiness. The employment outcome indicated that three of the participants had secured a job and another two participants had attained job offers. CONCLUSION: This pilot study suggested that a systematic vocational rehabilitation program can facilitate persons with occupational deafness to return to the workforce. Further research using a controlled investigation with larger sample size is recommended for evidence-based practice even though the results of this return-to-work program appear promising.

[Evaluation of hepatic resections in patients with benign and malignant lesions]. / Evaluación de las resecciones hepáticas en pacientes con lesiones benignas y malignas.

OBJECTIVE: To evaluate hepatic resections in patient with benign and malignant lesions during the years 1997 to 2002 at the Autonomous University Hospital Service of Maracaibo, Venezuela. MATERIALS: We carried out 18 hepatic resections for benign and/or malignant lesions; traumatic and infectious lesions were excluded. RESULTS: The female sex prevailed with 77.7% and age ranged between third and fourth decade. Three asymptomatic patients (16.6%) and 83.3% symptomatic; pain in upper hemiabdomen 80%. Ultrasonography was employed in 94.4% of cases, CT 83.3%, FNAB 27.7%, arteriography 22.2%, Echo-Doppler 16.6%, laparoscopy 11.1%, hepatic scintigraphy and ERCP, 5.5%. Minor resections represented 61.1%. Major hepatic resections represented 38.8%, with prevalence of left hepatectomy (33.3%). Blood loss was greatest in major hepatic resections with average of 1,242.9 +/- 827.8 ml. Pringles maneuver was carried out in 90% with duration average of 8.5 min; morbidity was 16.6%. Benign tumours prevailed with 66.6%. CONCLUSION: The technique of resection with intraparenchymatous identification of pedicles makes it the most successful technique for hepatic resections.

Oral complications in Chinese cancer patients undergoing chemotherapy.

In this study the oral status and mucositis-related symptoms in 94 Chinese cancer patients undergoing chemotherapy for with curative intent were examined. Data were collected by a research nurse at three stages over a period of 16 days: at the start, on day 8, and on day 16 of chemotherapy. The majority of subjects experienced a mild to moderate degree of mucositis with the highest score reported on day 8. Patients with head and neck cancer had consistently higher scores for mucositis and symptoms in most phases. Oral mucositis and symptoms were significantly and positively correlated with consumption of spicy and/or hot (temperature) food, and frequency of use of mouthwashes. Significantly higher scores were found in patients who: ate hot (temperature) food, had low tolerance for hot food and spicy foods, and were taking Chinese medicinal herbs. Careful monitoring of patient diet preferences, cancer site, and use of Chinese medicine is recommended for comprehensive oral assessment. The alleviation of oral mucositis and the associated symptoms through promotion of an appropriate food/fluid intake and more frequent use of mouthwashes should be emphasized in oral care.