Understanding ß-mannanase from Streptomyces sp. CS147 and its potential application in lignocellulose based biorefining.

Hydrolytic enzymes such as cellulase and hemicellulase have been attracted in lignocellulose based biorefinery. Especially, mannanase has been a growing interest in industrial applications due to its importance in the bioconversion. In this study, an extracellular endo-ß-1,4-D-mannanase was produced by Streptomyces sp. CS147 (Mn147) and purified 8.5-fold with a 43.4% yield using Sephadex G-50 column. The characterization of Mn147 was performed, and the results were as follows: molecular weight of ∼25 kDa with an optimum temperature of 50°C and pH of 11.0. The effect of metal ions and various reagents on Mn147 was strongly activated by Ca(+2) but inhibited by Mg(+2) , Fe(+2) , hydrogen peroxide, EDTA and EGTA. Km and Vmax values of Mn147 were 0.13 mg/mL and 294 µmol/min mg, respectively, when different concentrations (3.1 to 50 mg/mL) of locust bean gum galactomannan were used as substrate. In enzymatic hydrolysis of heterogeneous substrate (spent coffee grounds), Mn147 shows a similar conversion compared to commercial enzymes. In addition, lignocellulosic biomass can be hydrolyzed to oligosaccharides (reducing sugars), which can be further utilized for the production of biomaterials. These results showed that Mn147 is attractive in quest of potential bioindustrial applications.

Nutritional characteristics and body mass index of children in the Commonwealth of the Northern Mariana Islands.

Children in the Commonwealth of the Northern Mariana Islands have been shown to have nutrient deficiencies, but data were estimated from a non-population-based sample. The current study is a cross-sectional assessment of 420 Commonwealth of the Northern Mariana Islands children, 6 months to 10 years old. Diet, height, and weight were measured. Dietary intake was collected with a 24-hour dietary recall and analyzed using the Pacific Tracker dietary assessment tool. Prevalence of dietary nutrient adequacy was estimated by the Estimated Average Requirement cut-point method. Fiber intake was one-third Adequate Intake (AI) and sodium intake was above the Tolerable Upper Intake Level for all ages. Calcium intake was half the AI for 4 to 8 years old, and less than half the AI for 9 to 10 years old. Meat/meat alternatives were double the recommendation, while all other food groups were below the recommendation for all ages. Prevalence of dietary nutrient adequacy for vitamin A, vitamin C, vitamin E, folate, magnesium, and phosphorus was lowest among 9- to 10-year-olds. Based on body mass index-for-age percentiles, 45% of 7- to 10-year-olds, 26% of 4- to 6-year-olds, and 25% of 2- to 3-year-olds were overweight or obese. Increasing whole grain, fruit, vegetable, and dairy intakes; reducing meat intakes and high-calorie foods and drinks; and increasing physical activity could improve nutrient intakes and body mass index status in this population.