Glycerol as a reference material for fecal fat quantitation using low-resolution time domain ¹H NMR spectroscopy.

OBJECTIVES: To assess glycerol as reference material for low-resolution time-domain (1)H NMR analysis of fecal fat. DESIGN AND METHODS: NMR analysis of fecal fat in stool samples with added glycerol was used to assess linearity, recovery, and relationship with NMR lipid signal. RESULTS: The study revealed for added glycerol excellent linearity (r=0.9998), recovery (101-104%), and linear relationship with simulated fecal fat content. CONCLUSIONS: Glycerol is an effective reference material for NMR fecal fat analysis.