Field evaluation of an avian risk assessment model.

We conducted two laboratory subacute dietary toxicity tests and one outdoor subacute dietary toxicity test to determine the effectiveness of the U.S. Environmental Protection Agency's deterministic risk assessment model for evaluating the potential of adverse effects to birds in the field. We tested technical-grade diazinon and its D.Z.N 50W (50% diazinon active ingredient wettable powder) formulation on Canada goose (Branta canadensis) goslings. Brain acetylcholinesterase activity was measured, and the feathers and skin, feet, and gastrointestinal contents were analyzed for diazinon residues. The dose-response curves showed that diazinon was significantly more toxic to goslings in the outdoor test than in the laboratory tests. The deterministic risk assessment method identified the potential for risk to birds in general, but the factors associated with extrapolating from the laboratory to the field, and from the laboratory test species to other species, resulted in the underestimation of risk to the goslings. The present study indicates that laboratory-based risk quotients should be interpreted with caution.

Decomposed gosling feet provide evidence of insecticide exposure.

Canada goose goslings were exposed to turf sprayed with DZN diazinon 50W application (2.24 kg a.i./ha). The control plot was subjected to a water application. One foot from each bird was placed outdoors for 7 d to decompose and the other foot was kept frozen. Diazinon residues were analyzed on both feet. Results showed that diazinon was detected from undecomposed and decomposed feet of the birds. Diazinon residues were below the level of detection (<0.01 ppm, a.i.) on the feet from the control goslings. Decomposed feet may be used for determining insecticide exposure when the traditional matrices are not available.