RESUMO
A large non-coding RNA, termed α-Fur, of ~1000 nt has been detected in the extreme acidophile Acidithiobacillus ferrooxidans encoded on the antisense strand to the iron-responsive master regulator fur (ferric uptake regulator) gene. A promoter for α-fur was predicted bioinformatically and validated using gene fusion experiments. The promoter is situated within the coding region and in the same sense as proB, potentially encoding a glutamate 5-kinase. The 3' termination site of the α-fur transcript was determined by 3' rapid amplification of cDNA ends to lie 7 nt downstream of the start of transcription of fur. Thus, α-fur is antisense to the complete coding region of fur, including its predicted ribosome-binding site. The genetic context of α-fur is conserved in several members of the genus Acidithiobacillus but not in all acidophiles, indicating that it is monophyletic but not niche specific. It is hypothesized that α-Fur regulates the cellular level of Fur. This is the fourth example of an antisense RNA to fur, although it is the first in an extreme acidophile, and underscores the growing importance of cis-encoded non-coding RNAs as potential regulators involved in the microbial iron-responsive stimulon.
Assuntos
Acidithiobacillus/genética , Proteínas de Bactérias/genética , RNA Antissenso/genética , Proteínas Repressoras/genética , Acidithiobacillus/crescimento & desenvolvimento , Acidithiobacillus/metabolismo , Sequência de Bases , Ordem dos Genes , Genes Bacterianos , Ferro/metabolismo , Dados de Sequência Molecular , Regiões Promotoras Genéticas , Enxofre/metabolismo , Transcrição GênicaRESUMO
Acidithiobacillus ferrooxidans is a Gram-negative bacterium that lives at pH 2 in high concentrations of soluble ferrous and ferric iron, making it an interesting model for understanding the biological mechanisms of bacterial iron uptake and homeostasis in extremely acid conditions. A candidate fur(AF) (Ferric Uptake Regulator) gene was identified in the A. ferrooxidans ATCC 23270 genome. Fur(AF) has significant sequence similarity, including conservation of functional motifs, to known Fur orthologues and exhibits cross-reactivity to Escherichia coli Fur antiserum. The fur(AF) gene is able to complement fur deficiency in E. coli in an iron-responsive manner. Fur(AF) is also able to bind specifically to E. coli Fur regulatory regions (Fur boxes) and to a candidate Fur box from A. ferrooxidans, as judged by electrophoretic mobility shift assays. Fur(AF) represses gene expression from E. coli Fur-responsive promoters fiu and fhuF when expressed at high protein levels. However, it increases gene expression from these promoters at low concentrations and possibly from other Fur-regulated promoters involved in iron-responsive oxidative stress responses.
Assuntos
Acidithiobacillus/genética , Proteínas de Bactérias/genética , Proteínas Repressoras/genética , Transativadores/genética , Acidithiobacillus/metabolismo , Motivos de Aminoácidos/genética , Proteínas de Bactérias/imunologia , Proteínas de Bactérias/metabolismo , Reações Cruzadas , DNA Bacteriano/química , DNA Bacteriano/genética , Ensaio de Desvio de Mobilidade Eletroforética , Escherichia coli/genética , Deleção de Genes , Teste de Complementação Genética , Dados de Sequência Molecular , Ligação Proteica , Proteínas Repressoras/imunologia , Proteínas Repressoras/metabolismo , Análise de Sequência de DNA , Homologia de Sequência de Aminoácidos , Transativadores/metabolismoRESUMO
Bacterial tyrosyl-tRNA synthetases occur in two large subfamilies, TyrRS and TyrRZ, that possess about 25% amino acid identity. Their amino-terminal region, the active site domain, is more conserved (>36% identity). The carboxy-terminal segment of these enzymes includes the tRNA binding domain and contains only few conserved residues. Replacement of three of these residues in Acidithiobacillus ferrooxidans TyrRZ revealed that S356 and K395 play roles in tRNA binding, while H306, a residue at the junction of the catalytic and tRNA binding domains, stabilizes the Tyr-AMP:TyrRZ complex. The replacement data suggest that conserved amino acids in A. ferrooxidans TyrRZ and Bacillus stearothermophilus TyrRS play equivalent roles in enzyme function.
