Contrasted resistance and resilience of two mangrove forests after exposure to long-term and short-term anthropic disturbances.

Mangroves, coastal forests under the influence of tides, are known to be very resilient when they face natural disturbances such as storms or tsunami. While they provide several ecological services, they are threatened by many anthropic pressures. The aim of this study was to assess and to compare the stability of two mangrove fringes defined by contrasted set of natural constraints and exposed to pretreated domestic wastewaters discharges. The in situ experimental system set up in Mayotte Island (Indian Ocean) allowed us to determine both the short-term (2 years) and the long-term (9 years) resistance and the resilience. We focused on vegetation and crabs, an essential component of mangroves fauna. Wastewater discharges induced increases in tree coverage, leaves productivity and pigment content, and a decrease in crab diversity and density. Within 2 years after the release of the disturbance, several parameters reach back control values indicating fast resilience. Our results notably emphasized the high stability of the mangrove fringe dominated by Rhizophora mucronata trees, which was both more resistant and more resilient. This makes this fringe more suitable for application purposes, such as outfall for domestic wastewaters treatment plants.

Limited impact of several years of pretreated wastewater discharge on fauna and vegetation in a mangrove ecosystem.

It was hypothesized that mangroves, tropical wetlands, could be used for the finishing treatment of domestic wastewaters. Our aim was to determine if a nutrient-stressed mangrove could tolerate long-term discharges of pretreated wastewater (PW). Since 2008, in an in situ experimental system set up in Mayotte Island (Indian Ocean), domestic PW are discharged into two impacted areas (675 m2) dominated by different species of mangrove trees. Anthropogenic inputs during > 4.5 years led to an increase in vegetation growth associated with an increase in leaf pigment content, leaf surface and tree productivity. A marked increase in tree mortality was observed. There was no effect on crabs and meiofauna densities, but significant modifications of community structures. These effects may be directly linked to PW inputs, or indirectly to the modifications of the environment associated with higher tree growth. However, our results indicate that there was no major dysfunction the ecosystem.

Response of three biofilm-forming benthic microorganisms to Ag nanoparticles and Ag+: the diatom Nitzschia palea, the green alga Uronema confervicolum and the cyanobacteria Leptolyngbya sp.

Although the industrial use of nanoparticles has increased over the past decade, the knowledge about their interaction with benthic phototrophic microorganisms in the environment is still limited. This study aims to characterize the toxic effect of ionic Ag+ and Ag nanoparticles (citrate-coated silver nanoparticles, AgNPs) in a wide concentration range (from 1 to 1000 µg L-1) and duration of exposure (2, 5 and 14 days) on three biofilm-forming benthic microorganisms: diatom Nitzschia palea, green algae Uronema confervicolum and cyanobacteria Leptolyngbya sp. Ag+ has a significant effect on the growth of all three species at low concentrations (1-10 µg L-1), whereas the inhibitory effect of AgNPs was only observed at 1000 µg L-1 and solely after 2 days of exposure. The inhibitory effect of both Ag+ and AgNPs decreased in the course of the experiments from 2 to 14 days, which can be explained by the progressive excretion of the exopolysaccharides and dissolved organic carbon by the microorganisms, thus allowing them to alleviate the toxic effects of aqueous silver. The lower impact of AgNPs on cells compared to Ag+ can be explained in terms of availability, internalization, reactive oxygen species production, dissolved silver concentration and agglomeration of AgNPs. The duration of exposure to Ag+ and AgNPs stress is a fundamental parameter controlling the bioaccumulation and detoxification in benthic phototrophic microorganisms.

Community structure and nutrient level control the tolerance of autotrophic biofilm to silver contamination.

Autotrophic biofilms are complex and fundamental biological compartments of many aquatic ecosystems. Since microbial species differ in their sensitivity to stressors, biofilms have long been proposed for assessing the quality of aquatic ecosystems. Among the many stressors impacting aquatic ecosystems, eutrophication and metal pollution are certainly the most common. Despite that these stressors often occur together, their effects on biofilms have been far much studied separately than interactively. In this study, we evaluated the interactive effects of silver (Ag), a reemerging contaminant, and phosphorus (P), a nutrient often associated with freshwater eutrophication, on the structure and functioning of two types of autotrophic biofilms, one dominated by diatoms and another one dominated by cyanobacteria. We hypothesized that P would alleviate the toxic effects of Ag, either directly, through the contribution of P in metal detoxification processes, or indirectly, through P-mediated shifts in biofilm community compositions and associated divergences in metal tolerance. Results showed that Ag impacted biofilm community structure and functioning but only at unrealistic concentrations (50 µg/L). P availability led to significant shifts in biofilm community composition, these changes being more pronounced in diatom- than those in cyanobacteria-dominated biofilm. In addition, P tended to reduce the impact of Ag but only for the cyanobacteria-dominated biofilm. More generally, our results highlight the preponderant role of the initial community structure and nutrient level on biofilm response to metallic pollutants.