Ultrasound-assisted enzymatic synthesis of galacto-oligosaccharides using native whey with two commercial ß-galactosidases: Aspergillus oryzae and Kluyveromyces var lactis.

Native whey obtained during casein micelle microfiltration was used as a novel source to produce galacto-oligosaccharides (GOS). Since the presence of macromolecules and other interferers reduces biocatalyst performance, this work evaluated the effect of different ultrasound processing conditions on GOS synthesis using concentrated native whey. Ultrasonic intensities (UI) below 11 W/cm2 tended to increase the activity in the enzyme from Aspergillus oryzae for several minutes but accelerated the inactivation in that from Kluyveromyces lactis. At 40 °C, 40 % w/w native whey, 70 % wave amplitude, and 0.6 s/s duty-cycle, a UI of 30 W/cm2 was achieved, and the increased specific enzyme productivity was similar to the values obtained with pure lactose (∼0.136 g GOS/h/mgE). This strategy allows for obtaining a product containing prebiotics with the healthy and functional properties of whey proteins, avoiding the required purification steps used in the production of food-grade lactose.

Xylan extraction from hardwoods by alkaline pretreatment for xylooligosaccharide production: A detailed fractionation analysis.

In the last decades, the production of value-added products from lignocellulosic biomass (LCB) has gained relevance. Xylans, which are the main hemicellulose compounds in LCB, may be extracted by alkaline pretreatment and employed for xylooligosaccharide (XOS) production. However, xylan extraction currently works as a black box due to the lack of characterization of the involved streams. Therefore, the appropriate operational conditions often remain unclear, especially in hardwoods. In this study, alkaline/thermal pretreatments at different operational conditions were evaluated for xylan extractions from Chilean Nothofagus species sawdust, determining the chemical compositions of the fractions at each step of the process. Results indicated that increasing alkali concentration (NaOH) leads to a higher xylan extraction, but also to high salt production during the acid neutralization step, decreasing xylan's purity and therefore XOS production. In this context, decreasing NaOH concentration and neutralizing it by membrane filtration, allow extracting xylans (62.5 %) of higher-purity (77 %).