RESUMO
BACKGROUND AND AIMS: Polymorphisms in DNA repair gene may alter an individual's DNA repair capacity and be associated with the risk of various cancers. This study was designed to investigate whether ERCC1 +262A/C and XPF -357A/C polymorphisms affect individual susceptibility to esophageal squamous cell carcinoma (ESCC) and gastric cardiac adenocarcinoma (GCA). METHODS: In 389 ESCC patients vs. 778 healthy controls and 262 GCA patients vs. 524 healthy controls in a high incidence region of northern China, ERCC1 +262A/C polymorphism and XPF -357A/C polymorphism were genotyped by the method of polymerase chain reaction ligase detection reaction (PCR-LDR) and polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis, respectively. RESULTS: Family history of upper gastrointestinal cancers (UGIC) may increase the risk of ESCC and GCA. Allelotype and genotype distributions of ERCC1 +262A/C and XPF -357A/C polymorphisms in ESCC and GCA patients were not significantly different from that in their respective controls (p >0.05). Compared with ERCC1 +262C/C genotype, A/A genotype decreased the risk of GCA in nonsmokers (age, gender and family history of UGIC adjusted odds ratio [OR] = 0.30, 95% confidence interval [CI] = 0.13-0.70). Neither the A/C nor the C/C genotype was associated with the overall risk of ESCC and GCA when compared with the XPF -357A/A genotype. CONCLUSIONS: ERCC1 +262A/A genotype may reduce the risk of GCA for nonsmokers. XPF -357A/C polymorphism was not associated with the risk of ESCC and GCA in a population of a high-incidence region in northern China.
Assuntos
Adenocarcinoma/genética , Cárdia/patologia , Proteínas de Ligação a DNA/genética , Endonucleases/genética , Polimorfismo de Nucleotídeo Único , Neoplasias Gástricas/genética , Adenocarcinoma/enzimologia , Adenocarcinoma/epidemiologia , Idoso , Sequência de Bases , Carcinoma de Células Escamosas/enzimologia , Carcinoma de Células Escamosas/epidemiologia , Carcinoma de Células Escamosas/genética , Estudos de Casos e Controles , China/epidemiologia , Análise Mutacional de DNA , Neoplasias Esofágicas/enzimologia , Neoplasias Esofágicas/epidemiologia , Neoplasias Esofágicas/genética , Feminino , Estudos de Associação Genética , Predisposição Genética para Doença , Humanos , Incidência , Masculino , Pessoa de Meia-Idade , Dados de Sequência Molecular , Fatores de Risco , Fumar , Neoplasias Gástricas/enzimologia , Neoplasias Gástricas/epidemiologiaRESUMO
OBJECTIVE: To observe therapeutic effect of scalp-acupuncture plus body-acupuncture on spastic cerebral palsy and its effects on bone density and blood trace elements Ca, Cu, Zn, and Fe in the children with spastic cerebral palsy. METHODS: Eighty children of spastic cerebral palsy were randomly divided into an observation group and a control group, 40 cases in each group. The observation group were treated with scalp-acupuncture plus body-acupuncture combined with body therapy and performance therapy, and the control group with simple body therapy and performance therapy, once each day, for 3 courses, 30 days constituting one course. Changes of bone density and the trace elements were compared before and after treatment. RESULTS: The total effective rate was 87.5% in the observation group and 67.5% in the control group with a significant difference between the two groups (P < 0.05); after treatment, the bone density and blood Ca, Cu, Zn and Fe contents in the two groups significantly increased (P < 0.05, P < 0.01) with the observation group being superior to the control group (P < 0.05, P < 0.01). CONCLUSION: The scalp-acupuncture plus body-acupuncture combined with body therapy and performance therapy can improve bone density and contents of trace elements in the children of spastic cerebral palsy, and has a definite therapeutic effect on spastic cerebral palsy.
