A highly sensitive ratiometric fluorescence probe for sensing and imaging sulfite in food samples and living cells.

In this work, a ratiometric and chromogenic fluorescent probe 1 was synthesized for the detection of SO32-. The probe 1 at PBS (10 mM, pH = 7.4) presented a marked emission band at 661 nm. Upon addition of SO32- ions, a highly emissive adduct with a marked fluorescence at 471 nm were obtained through a Michael addition. The probe 1 displayed a noticeable fluorescence ratiometric response with a large shift (190 nm) in emission wavelength. The probe can quantitatively detect SO32- with high specificity, fast response (within 130 s) as well as low detection limit (13 nM), and a large Stokes shift (139 nm). Fluorescence imaging of HeLa cells indicated that 1 could be used for monitoring the intrinsically generated intracellular SO32- in living cells by ratiometric fluorescence imaging. Furthermore, 1 could be application in real water and sugar samples with high sensitivity and good recoveries.

Novel lysosome-targeted fluorescent molecular rotors based on a cyanine-like modular system and their application in living cells.

Two novel fluorescence molecular rotors DpIn and NaIn were designed and synthesized involving of indolium units linked with meta-diphenol or ortha-naphthalenediol moiety, respectively. They underwent intramolecular charge transfer to form a cyanine-like modular system at a physiological pH. In glycerol aqueous solutions, the probe DpIn exhibited NIR strong emission (3-fold) at ca. 700 nm, while the probe NaIn displayed a turn-on emission (8-fold) with a larger Stokes shift (⊿λ ≈ 97 nm). The HeLa cell imaging experiments indicated probe DpIn and NaIn both exhibited excellent selectivity for staining intracellular lysosomes instead of mitochondria. 1H NMR spectra revealed that more electrons were accumulated around benzene ring of indolium groups, which could be the evidence for its basic character leading to the lysosomes targeted staining. Furthermore, the probe NaIn proved to be an ideal lysosome-targeting tracer for monitor the changes of viscosity caused by stimuli in living cells.

[Auditory brainstem response testing revisit of infants who failed the hearing screening].

OBJECTIVE: Analyze the ABR testing results of local infants who failed the hearing screening and summarize its characteristics. METHOD: Carry on the first ABR test to 140 infants who failed the newborns' hearing screening when they are about 3-month age, the unusualities carry on the second ABR test at about 6-month age, and carry on the statistical analysis. RESULT: One hundred and forty infants with the first ABR diagnosis, 65 infants are normal, accounting for 46.43%; 75 infants have single or binaural ear hearing disorder, accounting for 53.57%, single ear 17.14%, binaural ear 36.43%, of which mild hearing disorder takes most, accounting for 27.14%, the profound hearing loss is also not extremely rare, accounting for 14.29%. Between male and female hearing disorder disease incidence rate difference is not remarkable statistics significance (P > 0.05). Between the number of high-risk in the hearing injury and non-high-risk after the comparison difference is not remarkable statistical significance (P > 0.05). With the population of above severe loss in hearing high-risk has the remarkable statistical significance with the non-high-risk comparison difference (P < 0.05). The second ABR subsequently visit 38 infants, 8 infants normal, total normal rate 52.14% (73/140), 16 infants mild hearing disorder 10 infants transfer normal either the change for the better, 6 infants do not have the change or the aggravation, 3 infants moderate hearing disorder 1 infant changes for the better, 2 infants do not have the change, 9 infants severe hearing disorder 3 infants have the change for the better, 3 infants do not have the change, 3 infants aggravate, 10 infants profound hearing disorder 9 infants do not have the change or the aggravation extremely, 1 example brain paralysis profound hearing disorder extension converges normally extremely. The non-high-risk whose hearing changes for the better is obviously higher than high-risk (P < 0.05). CONCLUSION: Should develop newborns' hearing screening comprehensively with key attention to high-risk loss of hearing. The severe hearing disorder has the variability, and so on exceptional child hearing situation appraisal should be prudent to the brain paralysis; should establish perfect tracing, revisiting and managing system as soon as possible.