Burial of microplastics in freshwater sediments facilitated by iron-organo flocs.

Microplastics are ubiquitous in standing freshwater bodies, consequently lakes and reservoirs may be important sinks for these contaminants. However, the mechanisms governing the deposition of microplastics and their interactions with the sediments are understudied. We demonstrate how aggregation-based transport facilitates the sinking and infiltration of buoyant microplastics into freshwater reservoir sediments by employing experiments with intact sediment cores. Buoyant polyethylene microplastics were rapidly (1-4 h) incorporated into sinking iron-organic aggregates, followed by swift deposition into sediments. Ingression of microplastic bearing flocs into sediments was completed within 6 days and led to stable deposition of the incorporated particles for at least 2 months. Most microplastics were deposited in the top 2 cm of the sediments and few particles (5-15%) were re-released into the water. Our results show at least 85% burial of microplastics, indicating the significant role of freshwaters with low flow velocities in reducing microplastic loads to the oceans.

Interaction of cyanobacteria with calcium facilitates the sedimentation of microplastics in a eutrophic reservoir.

Low-density microplastics are frequently found in sediments of many lakes and reservoirs. The processes leading to sedimentation of initially buoyant polymers are poorly understood for inland waters. This study investigated the impact of biofilm formation and aggregation on the density of buoyant polyethylene microplastics. Biofilm formation on polyethylene films (4 × 4 × 0.15 mm) was studied in a eutrophic reservoir (Bautzen, Saxony, Germany). Additionally, aggregation dynamics of small PE microplastics (~85 µm) with cyanobacteria were investigated in laboratory experiments. During summer phototrophic sessile cyanobacteria (Chamaesiphon spp. and Leptolyngbya spp.) precipitated calcite while forming biofilms on microplastics incubated in Bautzen reservoir. Subsequently the density of the biofilms led to sinking of roughly 10% of the polyethylene particles within 29 days of incubation. In the laboratory experiments planktonic cyanobacteria (Microcystis spp.) formed large and dense cell aggregates under the influence of elevated Ca2+ concentrations. These aggregates enclosed microplastic particles and led to sinking of a small portion (~0.4 %) of polyethylene microplastics. This study showed that both sessile and planktonic phototrophic microorganisms mediate processes influenced by calcium which facilitates densification and sinking of microplastics in freshwater reservoirs. Loss of buoyancy leads to particle sedimentation and could be a prerequisite for the permanent burial of microplastics within reservoir sediments.

Biofouling, metal sorption and aggregation are related to sinking of microplastics in a stratified reservoir.

Microplastic particles entering aquatic systems are rapidly colonized by microbial biofilms. The presence of microbial biomass may cause sinking of particles and as a consequence prevent their transport to the oceans. We studied microbial colonization of different polymer particles exposed in the epi-, meta- and hypolimnion of a freshwater reservoir during late summer for 47 days. Parameters measured included biofilm formation, metal sorption and sinking velocities. Microbial biofilms contained bacteria, cyanobacteria and algae as well as inorganic particles such as iron oxides. Regardless of biofilm thickness and biovolumes of different biofilm constituents, single polyethylene (PE) particles stayed buoyant, whereas the sinking velocity of single polystyrene (PS) and polyethylene terephthalate (PET) particles did not change significantly compared to initial values. During exposition, a mixing event occurred, by which anoxic, iron-rich water from the hypolimnion was mixed with water from upper layers. This induced aggregation and sinking of hypolimnetic PE particles together with organic matter, cyanobacteria colonies and iron minerals.