White mold resistance-associated quantitative trait loci in the Jalo x Small White common bean population.

We aimed to identify simple sequence repeat (SSR) markers linked to quantitative trait loci (QTLs) associated with white mold resistance in a segregating population derived from a cross between common bean cultivars Jalo and Small White, in the Southern State of Minas Gerais. Parents were crossed to obtain the F2 generation of 190 plants. From these, F2:3 and F2:4 progenies were obtained for phenotypic evaluation. DNA was extracted from F2 plants and parents for genotyping with SSR primers. For phenotypic assessment by the straw test, we used 190 F2:3 progenies and six lines in a triple-lattice design of 14 x 14 m. Nine trials were conducted for phenotyping by the oxalic acid method to evaluate 177 F2:4 progenies, in addition to the two parents and two common treatments represented by the Jalo and Corujinha lines. The experimental design for the oxalic acid method was completely randomized with three replicates. Adjusted means of evaluations related to F2:3 and F2:4 were used to identify QTLs by using the moving away method from the marker under Bayesian analysis. The markers GATS91, BM197, and X60000 stood out with high heritability and with effects on disease reduction by the straw test; therefore, these markers are promising for selection. The markers BM183, BM189, and SSR-IAC143 were associated with the QTLs identified by oxalic acid, providing greater effects in white mold resistance with high heritability. Based on the oxalic acid and straw test methods, the most prominent marker was PVBR189.

Isolation of clinically relevant fungal species from solid waste and environment of dental health services.

AIMS: This study was undertaken to detect, identify and determine antifungal susceptibility of yeast strains isolated from dental solid waste and to evaluate airborne fungi in the Brazilian dental health care environment and in the waste storage room. METHODS AND RESULTS: A group of 17 yeast strains were identified by macroscopic and microscopic characteristics, API 20C Aux system and Multiplex PCR. All 104 airborne fungal colonies were identified by macroscopic and microscopic morphology. The CLSI broth microdilution method was utilized as the susceptibility test. Candida parapsilosis was the prevailing yeast species recovered from waste, followed by Rhodotorula glutinis. Three strains of Candida guilliermondii presented minimal inhibitory concentration values considered to be susceptible dose dependent (2 µg ml(-1)) to voriconazole. Of all airborne fungal species, 69% were recovered from the waste storage room and 31% were recovered from the clinical/surgical environment. Most of them were identified as Cladosporium spp. CONCLUSIONS: These findings reinforce the potential risk of waste handling and point out the need for safe management to minimize the spread of these agents to the environment. Filamentous fungi isolation in almost all sampled environments indicates that a periodic monitoring of airborne microbiota in the dental health care service environment is required. SIGNIFICANCE AND IMPACT OF THE STUDY: The survival of yeast strains for 48 h suggests that dental waste should be carefully controlled and monitored.

Epidemiological survey of the enteropathogenic Escherichia coli isolated from children with diarrhoea.

Escherichia coli was isolated in 382 (94 per cent) of 406 children from 0 to 3 years of age who had been hospitalized for diarrhoea at the Hospital Municipal Salles Neto, Rio de Janeiro. Enteropathogenic Escherichia coli strains were isolated in 67 samples (18 per cent), distributed among the serogroups that were tested as follows: 0111 (33 per cent); 0125 (19 per cent); 0126, 0127, and 0142 (9 per cent); 0128 and 0119 (8 per cent); 055 (5 per cent); 0114 (2 per cent). No strains of EPEC belonging to serogroups 086, 0126, and 0158 were found. Among the samples in which EPEC strains were isolated, 15.0 per cent were children living in dwellings which had piped systems of water supply and drain, whereas with regard to those living in houses without such facilities, this percentage raised to 24 per cent. Similar results were found when the availability of water supply of drainage were taken separately.

A cura de objetivo minimo. Uma experiencia em psicoterapia breve na adolescencia. / The cure of minimal objective. An experience in brief psychotherapy in adolescence

O presente trabalho foi realizado pela equipe do setor de adolescentes do Instituto de Psiquiatria da UFRJ. A ideologia do setor e ter uma equipe de profissionais treinados para atender a imperiosa demanda das diversas patologias da populacao que acorre a instituicao. Para isto, desenvolve um amplo espectro de tecnicas de atendimento: psicoterapia breve individual, psicoterapia breve de grupo, psicoterapia familiar breve, co-terapia, psicofarmacoterapia etc. O objetivo principal desta comunicacao e apresentar um pequeno modelo empirico, instrumentado na psicoterapia breve da adolescencia. Deste ponto de vista, tenta-se demonstrar que uma das dificuldades para o trabalho clinico reside em uma serie de ideias preconcebidas, que obstaculizam as tarefas assistenciais. Estas ideias funcionam como mitos, que impedem ou atrapalham o desenvolvimento da psicoterapia. Desenham-se alguns destes mitos, e se tenta ilustrar, mediante tres casos clinicos, o que nos chamamos de "cura de objetivo minimo" (C.O.M.).Esta cura de objetivo minimo consiste em tentar resolver, mediante abordagens multivariadas de ordem empirica que se vao apresentando.Neste sentido, o trabalho pretende mostrar que a resolucao de qualquer resistencia ou obstaculo ao tratamento e uma cura minima.resolucao de qualquer resistencia ou obstaculo ao tratamento e uma cura minima